Functional analysis of cartilage glycans for the elucidation of osteoarthritis pathomechanisms

• Project/Area Number: 16K15650
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Hokkaido University
• Principal Investigator: Iwasaki Norimasa 北海道大学, 医学研究科, 教授 (30322803)
• Co-Investigator(Kenkyū-buntansha): 小野寺 智洋 北海道大学, 大学病院, 講師 (70547174)
• Research Collaborator: HOMAN Kentaro 北海道大学, 大学院医学研究院, 博士研究員 (40823331)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 骨・軟骨代謝 / 糖鎖 / 骨・軟骨代謝学

Outline of Final Research Achievements

Our hypothesis was that early osteoarthritic cahnges induced by alterations in cartilage glycans would be reversible. To test this hypothesis, we created in vivo and in vitro early osteoarthritic models based on alpha-mannosidase administration. The administration of alpha-mannosidase induced early osteoarthritis in in vivo and in vitro models. Additionally, this histological changes were recovered by the discontinuation of alpha-mannosidase adminiatrations. Future studies will be performed to clarify this recovery mechanism in early osteoarthritis pathogenesis.

Research Products

• [Presentation] 軟骨細胞肥大分化における統合グライコミクス (2018)
  • Author(s): 宝満健太郎、小野寺智洋、花松久寿、古川潤一、上徳善太、松原新史、菱村亮介、金佑泳、濱崎雅成、徐亮、宮崎拓自、田園、岩崎倫政
  • Organizer: 第31回 日本軟骨代謝学会
• [Presentation] The Candidates of Glyco-Biomarker of Chondrocyte Hypertrophy Detected by Comprehensive N-glycan Profiling (2018)
  • Author(s): Homan K, Onodera T, Furukawa J, Momma D, Matsuoka M, Baba R, Hontani K, Joutoku Z, Matsubara S, Hishimura R, Kim WY, Hamasaki M, Xu L, Tian Y, Iwasaki N
  • Organizer: Orthopaedic Research Society 2018 Annual Meeting
• [Presentation] The enzymatic cleavage of high-mannose type N-glycan induces recoverable cartilage degradation (2017)
  • Author(s): Homan K, Onodera T, Furukawa J, Momma D, Matsuoka M, Baba R, Hontani K, Joutoku Z, Matsubara S, Hishimura R, Kim WY, Hamasaki M, Xu L, Iwasaki N
  • Organizer: ORS 2017 Annual Meeting
  • Place of Presentation: San Diego、US
  • Year and Date: 2017-03-19
  • Int'l Joint Research
• [Presentation] 高マンノース型糖鎖の減少は可逆的軟骨変性を引き起こす (2017)
  • Author(s): 宝満健太郎、小野寺智洋、古川潤一、門間太輔、松岡正剛、馬場力哉、本谷和俊、上徳善太、松原新史、菱村亮介、金佑泳、濱崎雅成、徐亮、田園、岩崎倫政
  • Organizer: 第30回 日本軟骨代謝学会
  • Place of Presentation: みやこめっせ（京都府京都市）
  • Year and Date: 2017-03-03
• [Presentation] 高マンノース型糖鎖の変化は早期OAに類似した可逆的軟骨変性を生じさせる (2017)
  • Author(s): 宝満健太郎、小野寺智洋、古川潤一、門間太輔、松岡正剛、馬場力哉、本谷和俊、上徳善太、松原新史、菱村亮介、金佑泳、濱崎雅成、除亮、田園、岩崎倫政
  • Organizer: 第132回 北海道整形災害外科学会
  • Place of Presentation: 札幌医科大学（北海道札幌市）
  • Year and Date: 2017-01-28