Myogenic potency of human satellite cells from torn muscles

• Project/Area Number: 16K15651
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Tohoku University
• Principal Investigator: Hagiwara Yoshihiro 東北大学, 医学系研究科, 准教授 (90436139)
• Co-Investigator(Kenkyū-buntansha): 神崎 展 東北大学, 医工学研究科, 准教授 (10272262) ; 土谷 昌広 東北福祉大学, 健康科学部, 准教授 (60372322)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 筋前駆細胞 / 脂肪浸潤 / 関節鏡手術 / 腱板断裂 / 脂肪前駆細胞 / 筋細胞 / FACS / DNAマイクロアレイ / 筋衛星細胞 / 筋断裂 / 再生 / 筋再生 / 脂肪変性 / 収縮筋培養

Outline of Final Research Achievements

Rotator cuff tear is a common disease in elderly patients that leads to shoulder weakness, pain, and disability in the activities of daily living. Some patients with massive rotator cuff tears have an unacceptable clinical outcome because of muscle atrophy and fatty infiltration. The satellite cell has central role of muscle regeneration, however, there are few reports about human muscle. The purpose of this study was to compare in vivo features of human myogenic and adipogenic precursors in the rotator cuff muscles in both torn supraspinatus (SSP) and intact subscapularis (SSC) tendons. The human satellite cells from the SSP muscle with fatty infiltration maintained intrinsic myogenic potentials.

Research Products

• [Journal Article] Retained Myogenic Potency of Human Satellite Cells from Torn Rotator Cuff Muscles Despite Fatty Infiltration (2018)
  • Author(s): Koide M, Hagiwara Y, Tsuchiya M, Kanzaki M, Hatakeyama H, Tanaka Y, Minowa T, Takemura T, Ando A, Sekiguchi T, Yabe Y, Itoi E.
  • Journal Title: The Tohoku Journal of Experimental Medicine Volume: 244 Issue: 1 Pages: 15-24
  • DOI: 10.1620/tjem.244.15
  • NAID: 130006287941
  • ISSN: 0040-8727, 1349-3329
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 腱板断裂における脂肪変性を伴う筋組織中のヒト筋衛星細胞の分化 (2017)
  • Author(s): 小出将志、萩原嘉廣、安藤晃、関口拓矢、金澤憲治、土谷昌広、神崎展、井樋栄二
  • Organizer: 第44回日本肩関節学会
• [Presentation] 廃用萎縮・脂肪変性した筋組織中の筋・脂肪前駆細胞の分化能の評価 (2016)
  • Author(s): 小出将志、萩原嘉廣、安藤晃、矢部裕、金澤憲治、関口拓矢、板谷信行、吉田新一郎、神崎展、畠山裕康、土谷昌広、井樋栄二
  • Organizer: 第31回日本整形外科学会基礎学術集会
  • Place of Presentation: 福岡、福岡国際会議場
  • Year and Date: 2016-10-13