Investigation of mechanism of bone metastasis and the novel treatment of metastatic bone tumor
Project/Area Number |
16K15658
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Orthopaedic surgery
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Research Institution | Kanazawa University |
Principal Investigator |
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Project Period (FY) |
2016-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | 骨転移 / 蛍光タンパク / 腫瘍切除術 / 蛍光イメージング / 蛍光ガイド下手術 / がん / 癌 / 骨髄 / 蛍光蛋白 / 転移 |
Outline of Final Research Achievements |
The present report demonstrates efficacy of fluorescence-guided surgery (FGS) to resect and prevent recurrence of experimental skeletal metastasis in a nude-mouse model of human prostate cancer. Green fluorescent protein (GFP)-expressing PC-3 human prostate cancer cells were injected into the intramedullary cavity of the tibia in 25 nude mice. One week after implantation, monoclonal antibodies, specific for carcinoembryonic antigen (CEA), labeled with DyLight 650, were injected into the tail vein of 13 mice. Thirteen mice underwent FGS and 12 mice underwent bright-light surgery (BLS). Weekly GFP fluorescence imaging of the mice was performed to observe tumor recurrence. FGS group showed only slight tumor growth and significantly improved disease-free survival of the treated mice. Our study demonstrated that FGS significantly reduced residual tumor as well as the recurrence of experimental prostate-cancer bone metastasis.
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Report
(3 results)
Research Products
(99 results)
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[Journal Article] Anti-tumor effects of a nonsteroidal anti-inflammatory drug zaltoprofen on chondrosarcoma via activating peroxisome proliferator-activated receptor gamma and suppressing matrix metalloproteinase-2 expression.2018
Author(s)
Higuchi T, Takeuchi A, Munesue S, Yamamoto N, Hayashi K, Kimura H, Miwa S, Inatani H, Shimozaki S, Kato T, Aoki Y, Abe K, Taniguchi Y, Aiba H, Murakami H, Harashima A, Yamamoto Y, Tsuchiya H.
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Journal Title
Cancer Med.
Volume: 印刷中
Issue: 5
Pages: 1944-1954
DOI
NAID
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Peer Reviewed / Open Access
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[Journal Article] Tumor-targeting Salmonella typhimurium A1-R combined with recombinant methioninase and cisplatinum eradicates an osteosarcoma cisplatinum-resistant lung metastasis in a patient-derived orthotopic xenograft (PDOX) mouse model: Decoy, trap and kill chemotherapy moves toward the clinic2018
Author(s)
Igarashi K, Kawaguchi K, Kiyuna T, Miyake K, Miyake M, Li S, Han Q, Tan Y, Zhao M, Li Y, Nelson SD, Dry SM, Singh AS, Elliott IA, Russell TA, Eckardt MA, Yamamoto N, Hayashi K, Kimura H, Miwa S, Tsuchiya H, Eilber FC, Hoffman RM
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Journal Title
Cell Cycle
Volume: 印刷中
Issue: 6
Pages: 801-809
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Recombinant methioninase in combination with doxorubicin (DOX) overcomes first-line DOX resistance in a patient-derived orthotopic xenograft nude-mouse model of undifferentiated spindle-cell sarcoma2018
Author(s)
Igarashi K, Kawaguchi K, Li S, Han Q, Tan Y, Murakami T, Kiyuna T, Miyake K, Miyake M, Singh AS, Eckardt MA, Nelson SD, Russell TA, Dry SM, Li Y, Yamamoto N, Hayashi K, Kimura H, Miwa S, Tsuchiya H, Singh SR, Eilber FC, Hoffman RM
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Journal Title
Cancer Lett
Volume: 417
Pages: 168-173
DOI
Related Report
Peer Reviewed
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[Journal Article] Growth of doxorubicin-resistant undifferentiated spindle-cell sarcoma PDOX is arrested by metabolic targeting with recombinant methioninase2018
Author(s)
Igarashi K, Li S, Han Q, Tan Y, Kawaguchi K, Murakami T, Kiyuna T, Miyake K, Li Y, Nelson SD, Dry SM, Singh AS, Elliott IA, Russell TA, Eckardt MA, Yamamoto N, Hayashi K, Kimura H, Miwa S, Tsuchiya H, Eilber FC, Hoffman RM
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Journal Title
J Cell Biochem
Volume: 119
Issue: 4
Pages: 3537-3544
DOI
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Peer Reviewed
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[Journal Article] Effective Metabolic Targeting of Human Osteosarcoma Cells In Vitro and in Orthotopic Nude-mouse Models with Recombinant Methioninase2017
Author(s)
Igarashi K, Kawaguchi K, Kiyuna T, Miyake K, Murakami T, Yamamoto N, Hayashi K, Kimura H, Miwa S, Tsuchiya H, Hoffman RM
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Journal Title
Anticancer Res
Volume: 37
Pages: 4807-4812
Related Report
Peer Reviewed
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[Journal Article] Efficacy In Vitro of Caffeine and Valproic Acid on Patient-Derived Undifferentiated Pleomorphic Sarcoma and Rhabdomyosarcoma Cell Lines2017
Author(s)
Igarashi K, Kawaguchi K, Kiyuna T, Murakami T, Miwa S, Nelson SD, Dry SM, Li Y, Singh AS, Kimura H, Hayashi K, Yamamoto N, Tsuchiya H, Eilber FC, Hoffman RM
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Journal Title
Anticancer Res
Volume: 37
Pages: 4081-4084
Related Report
Peer Reviewed
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[Journal Article] Pathogenesis of Osteosclerotic Change Following Treatment with an Antibody Against RANKL for Giant Cell Tumour of the Bone2017
Author(s)
Rosario M, Takeuchi A, Yamamoto N, Hayashi K, Miwa S, Higuchi T, Abe K, Taniguchi Y, Aiba H, Tanzawa Y, Murakami H, Tsuchiya H
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Journal Title
Anticancer Res
Volume: 37
Pages: 749-754
Related Report
Peer Reviewed
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[Journal Article] Do Mesenchymal Stem Cells Derived From Atypical Lipomatous Tumors Have Greater Differentiation Potency Than Cells From Normal Adipose Tissues?2017
Author(s)
Inatani H, Yamamoto N, Hayashi K, Kimura H, Takeuchi A, Miwa S, Higuchi T, Abe K, Taniguchi Y, Yamada S, Asai K, Otsuka T, Tsuchiya H.
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Journal Title
Clin Orthop Relat Res
Volume: 印刷中
Issue: 6
Pages: 1693-1701
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Patient-derived orthotopic xenograft (PDOX) mouse model of adult rhabdomyosarcoma invades and recurs after resection in contrast to the subcutaneous ectopic model2017
Author(s)
Igarashi K, Kawaguchi K, Kiyuna T, Murakami T, Miwa S, Nelson SD, Dry SM, Li Y, Singh A, Kimura H, Hayashi K, Yamamoto N, Tsuchiya H, Eilber FC, Hoffman RM
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Journal Title
Cell Cycle
Volume: 16
Issue: 1
Pages: 91-94
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Surgical management of proximal fibular tumors: A report of 12 cases2016
Author(s)
Inatani H, Yamamoto N, Hayashi K, Kimura H, Takeuchi A, Miwa S, Higuchi T, Abe K, Taniguchi Y, Yamada S, Okamoto H, Otsuka T, Tsuchiya H
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Journal Title
J Bone Oncol
Volume: 5
Issue: 4
Pages: 163-166
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Efficacy of the Combination of a PARP Inhibitor and UVC on Cancer Cells as Imaged by Focus Formation by the DNA Repair-related Protein 53BP1 Linked to Green Fluorescent Protein2016
Author(s)
Tome Y, Uehara F, Miwa S, Yano S, Mii S, Efimova EV, Bouvet M, Kimura H, Tsuchiya H, Kanaya F, Hoffman RM
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Journal Title
Anticancer Res
Volume: 36
Pages: 3821-6
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] The outcomes of reconstruction using frozen autograft combined with iodine-coated implants for malignant bone tumors: compared with non-coated implants2016
Author(s)
Shirai T, Tsuchiya H, Terauchi R, Tsuchida S, Mizoshiri N, Igarashi K, Miwa S, Takeuchi A, Kimura H, Hayashi K, Yamamoto N, Kubo T
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Journal Title
Jpn J Clin Oncol
Volume: 46
Issue: 8
Pages: 735-40
DOI
NAID
Related Report
Peer Reviewed
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