Screening for alternative transcription initiation of tyrosine kinase genes in bone and soft tissue tumors

• Project/Area Number: 16K15670
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Juntendo University
• Principal Investigator: Suehara Yoshiyuki 順天堂大学, 医学部, 准教授 (70509405)
• Co-Investigator(Kenkyū-buntansha): 高阪 真路 国立研究開発法人国立がん研究センター, 研究所, 主任研究員 (00627119) ; 小林 英介 国立研究開発法人国立がん研究センター, 中央病院, 医長 (40365292)
• Research Collaborator: ENDO Makoto ; SAITO Tsuyoshi ; HAYASHI Takuo ; KUBOTA Daisuke ; MUKAIHARA Kenta ; AKAIKE Keisuke ; TANABE Yu ; ISHII Midori
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 骨軟部腫瘍 / 軟部肉腫 / 融合遺伝子 / 遺伝子転写変異体 / 遺伝子変異体 / tyrosine Kinase / TK遺伝子転写変異体 / TK遺伝子変異 / 転写変異体 / チロシンキナーゼ

Outline of Final Research Achievements

Soft tissue sarcomas (STSs) are a heterogeneous group of rare malignant tumors. Systemic chemotherapy consisting cytotoxic anticancer agents for STS contributes on improving prognosis of STS treatment. However, advanced STSs have still poor prognosis and effective systemic therapies have not yet been established. Therefore, the identification of novel therapeutic targets and novel effective therapies are required. Receptor tyrosine kinases (RTKs) are being increasingly used as therapeutic targets for a variety of cancers, especially RTK fusion genes. A recent report found that the novel TK transcript initiates from a de novo alternative transcription initiation (ATI) site, especially in ALK. In addition, the TK-ATI has a novel mechanism of oncogene activation in cancer and TK inhibitors could suppress the kinase activity of TK-ATI. Based on these background, we conducted TK screening using NanoString to identify rational therapeutic TK targets including TK-fusions and TK-ATIs in STSs.

Academic Significance and Societal Importance of the Research Achievements

本研究は、難治性かつ「希少がん」である骨軟部腫瘍の生命予後改善の要となる新規分子治療標的の開発を行った。具体的には、他癌種において発見されているdriver-oncogeneである転写変異体について、骨軟部腫瘍の手術検体を用いて全TK遺伝子の探索を進めた。その方法として独自のNanostringの手法に基づいたTK遺伝子転写変異体・融合遺伝子探索システムを用い、それら同定TK遺伝子転写変異体・融合遺伝子については、骨軟部腫瘍における癌化機能の解明とそのターゲットとなるTK阻害剤奏功性の検証を進め、TK阻害剤の適応拡大を含めた新規分子治療標的の開発を行い、骨軟部腫瘍の予後改善へ貢献を目的とした。

Research Products

• [Int'l Joint Research] Memorial Sloan Kettering Cancer Center/Department of Pathology/Marc Ladanyi(米国)
• [Int'l Joint Research] メモリアルスローンケタリングがんセンター(米国)
• [Int'l Joint Research]
• [Int'l Joint Research] Memorial Sloan-Kettering Cancer Center(米国)
• [Journal Article] Comprehensive assay for the molecular profiling of cancer by target enrichment from formalin‐fixed paraffin‐embedded specimens (2019)
  • Author(s): Kohsaka Shinji、Tatsuno Kenji、Ueno Toshihide、et al.
  • Journal Title: Cancer Science Volume: 110 Issue: 4 Pages: 1464-1479
  • DOI: 10.1111/cas.13968
  • Peer Reviewed / Open Access
• [Journal Article] Multicenter experience with large panel next-generation sequencing in patients with advanced solid cancers in Japan. (2019)
  • Author(s): Kato S, Hayashi T, Suehara Y, Hamanoue H, Yamanaka S, Ichikawa Y, Higurashi T, Ohashi K, Yamaguchi S, Nozaki Y, Terao Y, Saito T.
  • Journal Title: Jpn J Clin Oncol Volume: 49(2) Issue: 2 Pages: 174-182
  • DOI: 10.1093/jjco/hyy173
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Squamous cell carcinoma arising from chronic osteomyelitis massively expanding into the medullary cavity: A case report. (2019)
  • Author(s): Kurihara T, Suehara Y, Akaike K, Hayashi T, Okubo T, Kim Y, Takagi T, Kaneko K, Yao T, Saito T.
  • Journal Title: Human Pathology Case reports Volume: 16 Pages: 100288-100288
  • DOI: 10.1016/j.ehpc.2019.01.008
  • Peer Reviewed / Open Access
• [Journal Article] Fibrocartilaginous mesenchymoma of the tibia with predominant microcystic features: A case report and literature review (2019)
  • Author(s): Saito T, Motoi T, Suehara Y, Takagi T, Okubo T, Kurihara T, Hayashi T, Kudo T, Maruyama Y, Kaneko K, YaoT.
  • Journal Title: Human Pathology Case reports Volume: 16 Pages: 100288-100288
  • DOI: 10.1016/j.ehpc.2019.01.007
  • Peer Reviewed / Open Access
• [Journal Article] Proteomic signatures corresponding to the SS18/SSX fusion gene in synovial sarcoma. (2018)
  • Author(s): Ishii M, Suehara Y, Sano K, Kohsaka S, Hayashi T, Kazuno S, Akaike K, Mukaihara K, Kim Y, Okubo T, Takamochi K, Takahashi F, Kaneko K, Saito T.
  • Journal Title: Oncotarget Volume: 9(101) Issue: 101 Pages: 37509-37519
  • DOI: 10.18632/oncotarget.26493
  • Peer Reviewed / Open Access
• [Journal Article] PPP2R1A regulated by PAX3/FOXO1 fusion contributes to the acquisition of aggressive behavior in PAX3/FOXO1-positive alveolar rhabdomyosarcoma. (2018)
  • Author(s): Akaike K, Suehara Y, Kohsaka S, Hayashi T, Tanabe Y, Kazuno S, Mukaihara K, Toda-Ishii M, Kurihara T, Kim Y, Okubo T, Hayashi Y, Takamochi K, Takahashi F, Kaneko K, Ladanyi M, Saito T.
  • Journal Title: Oncotarget Volume: 9(38) Issue: 38 Pages: 25206-25215
  • DOI: 10.18632/oncotarget.25392
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Next-generation sequencing analysis for gastric adenocarcinoma with enteroblastic differentiation: emphasis on the relationship with hepatoid adenocarcinoma. (2018)
  • Author(s): 1.Akazawa Y, Saito T, Hayashi T, Yanai Y, Tsuyama S, Akaike K, Suehara Y, Takahashi F, Takamochi K, Ueyama H, Murakami T, Watanabe S, Nagahara A, Yao T.
  • Journal Title: Hum Pathol Volume: 78 Pages: 79-88
  • DOI: 10.1016/j.humpath.2018.04.022
  • Peer Reviewed / Open Access
• [Journal Article] KCTD12 is negatively regulated by Kit in gastrointestinal stromal tumors. (2018)
  • Author(s): Suehara Y, Akaike K, Mukaihara K, Kurisaki-Arakawa A, Kubota D, Okubo T, Mitomi H, Mitani K, Takahashi M, Toda-Ishii M1, Kim Y, Tanabe Y, Takagi T, Hayashi T, Mogushi K, Kaneko K, Yao T, Saito T.
  • Journal Title: Oncotarget Volume: 9(43) Issue: 43 Pages: 27016-27026
  • DOI: 10.18632/oncotarget.25469
  • Peer Reviewed / Open Access
• [Journal Article] IRE1α-XBP1 inhibitors exerted anti-tumor activities in Ewing's sarcoma. (2018)
  • Author(s): Tanabe Y, Suehara Y, Kohsaka S, Hayashi T, Akaike K, Mukaihara K, Kurihara T, Kim Y, Okubo T, Ishii M, Kazuno S, Kaneko K, Saito T.
  • Journal Title: Oncotarget Volume: 9(18) Issue: 18 Pages: 14428-14443
  • DOI: 10.18632/oncotarget.24467
  • Peer Reviewed / Open Access
• [Journal Article] Non-small cell lung carcinoma with diffuse co-expression of thyroid transcription factor-1 and ΔNp63/p40 (2018)
  • Author(s): Hayashi Takuo、Takamochi Kazuya、Yanai Yuka、Mitani Keiko、Tomita Hisashi、Mogushi Kaoru、Suehara Yoshiyuki、Takahashi Fumiyuki、Suzuki Kenji、Saito Tsuyoshi、Yao Takashi
  • Journal Title: Human Pathology Volume: S0046-8177 Pages: 30037-6
  • DOI: 10.1016/j.humpath.2018.01.023
  • Peer Reviewed / Open Access
• [Journal Article] Pediatric soft tissue tumor of the upper arm with LMNA-NTRK1 fusion (2018)
  • Author(s): Kohsaka Shinji、Saito Tsuyoshi、Akaike Keisuke、Suehara Yoshiyuki、Hayashi Takuo、Takagi Tatsuya、Kaneko Kazuo、Ueno Toshihide、Kojima Shinya、Kohashi Ken-ichi、Mano Hiroyuki、Oda Yoshinao、Yao Takashi
  • Journal Title: Human Pathology Volume: 72 Pages: 167-173
  • DOI: 10.1016/j.humpath.2017.08.017
  • Peer Reviewed / Open Access
• [Journal Article] A method of high-throughput functional evaluation of EGFR gene variants of unknown significance in cancer (2017)
  • Author(s): Kohsaka Shinji、Nagano Masaaki、Ueno Toshihide、Suehara Yoshiyuki、Hayashi Takuo、Shimada Naoko、Takahashi Kazuhisa、Suzuki Kenji、Takamochi Kazuya、Takahashi Fumiyuki、Mano Hiroyuki
  • Journal Title: Science Translational Medicine Volume: 9 Issue: 416 Pages: 6566-6566
  • DOI: 10.1126/scitranslmed.aan6566
  • Peer Reviewed / Open Access
• [Journal Article] DNA mismatch repair deficiency in surgically resected lung adenocarcinoma: Microsatellite instability analysis using the Promega panel (2017)
  • Author(s): Takamochi Kazuya、Takahashi Fumiyuki、Suehara Yoshiyuki、Sato Eiichi、Kohsaka Shinji、Hayashi Takuo、Kitano Shigehisa、Uneno Toshihide、Kojima Shinya、Takeuchi Kengo、Mano Hiroyuki、Suzuki Kenji
  • Journal Title: Lung cancer Volume: 110 Pages: 26-31
  • DOI: 10.1016/j.lungcan.2017.05.016
  • Peer Reviewed / Open Access
• [Journal Article] Cabozantinib and dastinib exert anti-tumor activity in alveolar soft part sarcoma (2017)
  • Author(s): Mukaihara K, Tanabe Y, Kubota D, Akaike K, Hayashi T, Mogushi K, Hosoya M, Sato S,Kobayashi E, Okubo T, Kohsaka S, Saito T, Kaneko K, Suehara Y.
  • Journal Title: PLoS One Volume: 12(9) Issue: 9 Pages: 0185321-0185321
  • DOI: 10.1371/journal.pone.0185321
  • Peer Reviewed / Open Access
• [Journal Article] Arterial Thoracic Outlet Syndrome (aTOS) and Cerebellar Infarction Following a Stress Fracture of the First Rib and Extensive Callus (2017)
  • Author(s): 7.Suehara Y, Imashimizu K, Miyamoto N, Uehara H, Tanabe Y, Hattori N, Suzuki K, Saito T, Kaneko K.
  • Journal Title: J Bone Joint Surg. Case Connector Volume: 3 Issue: 3 Pages: e64-e64
  • DOI: 10.2106/jbjs.cc.17.00001
  • Peer Reviewed
• [Journal Article] TERT promoter mutations are rare in bone and soft tissue sarcomas of Japanese patients. (2016)
  • Author(s): Saito T, Akaike K, Kurisaki A, Ishii M, Mukaihara K, Suehara Y, Takagi T, Kaneko K,
  • Journal Title: Molecular and Clinical Oncology. Volume: 4(1) Issue: 1 Pages: 61-64
  • DOI: 10.3892/mco.2015.674
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Protein Expression Profiling of Giant Cell Tumors of Bone Treated with Denosumab. (2016)
  • Author(s): Mukaihara K, Suehara Y, Kohsaka S, Akaike K, Tanabe Y, Kubota D, Ishii M, Fujimura T, Kazuno S, Okubo T, Takagi T, Yao T, Kaneko K, Saito T.
  • Journal Title: PLoS One Volume: 10 Issue: 2 Pages: e0148401-e0148401
  • DOI: 10.1371/journal.pone.0148401
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Expression of F-actin-capping protein subunit beta is associated with cell growth and motility in epithelioid sarcoma. (2016)
  • Author(s): Mukaihara K, Suehara Y*, Kohsaka S, Kubota D, Toda-Ishii M, Akaike K, Fujimura T, Kobayashi E, Yao T, Ladanyi, M, Kaneko K, Saito T.
  • Journal Title: BMC cancer. Volume: 16(1) Issue: 1 Pages: 206-206
  • DOI: 10.1186/s12885-016-2235-z
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] An Outpatient-based Survey on the Recognition of Locomotive Syndrome in Japan: The Results of three Years of Surveys. (2016)
  • Author(s): Tanabe Y, Suehara Y, Okubo T, Kim Y, Ishii M,Kawasaki T, Matsuoka K, Akaike K, Mukaihara K, Kubota D, Okubo N, Maruyama Y, Saito T, Kaneko K.
  • Journal Title: British Journal of Medicine & Medical Research. Volume: 15(9) Issue: 9 Pages: 1-9
  • DOI: 10.9734/bjmmr/2016/26127
  • Peer Reviewed / Open Access
• [Journal Article] Paget's sarcoma with sarcoma-specific TP53 mutation arising from a Japanese patient. (2016)
  • Author(s): Akaike K, Toda-Ishii M, Suehara Y, Takagi T, Kaneko K,Yao T, Saito T.
  • Journal Title: International journal of clinical and experimental pathology. Volume: 9(3) Pages: 3978-3986
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Large skin ulcer due to a subcutaneous orthopedic implant after bevacizumab therapy: (2016)
  • Author(s): Suehara Y*, Osawa H, Kubota D, Kato S, Akaike K, Tanabe Y, Saito T, Kaneko K.
  • Journal Title: J Bone Joint Surg Volume: 6 (3) Issue: 3 Pages: e70-e70
  • DOI: 10.2106/jbjs.cc.15.00281
  • Peer Reviewed / Open Access
• [Journal Article] Clinicopathological effects of protein phosphatase 2, regulatory subunit A, alpha mutations in gastrointestinal stromal tumors (2016)
  • Author(s): Ishii M, Akaike K, Suehara Y, Mukaihara K, Kubota K , Kohsaka S, Okubo T, Mitani K, Mogushi K, Takagi T, Kaneko K, Yao T, Saito T.
  • Journal Title: Modern Pathology. Volume: 29 Issue: 11 Pages: 1424-1432
  • DOI: 10.1038/modpathol.2016.138
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Reduced argininosuccinate synthetase expression in refractory sarcomas. (2016)
  • Author(s): Kim Y, Kobayashi E, Kubota D, Suehara Y, Mukaihara K, Akaike K, Ito A, Kaneko K, Chuman H, Kawai A, Kitano S.
  • Journal Title: Oncotarget. Volume: 7(43) Issue: 43 Pages: 70832-70844
  • DOI: 10.18632/oncotarget.12225
  • Peer Reviewed / Open Access
• [Journal Article] A case report of using nivolumab for a malignant melanoma patient with rheumatoid arthritis (2016)
  • Author(s): Kageyama S, Yamaguchi S, Ito S, Suehara Y, Saito T, Akaike K, Miura K, Kato S.
  • Journal Title: Int Canc Conf J. Volume: 5(4) Issue: 4 Pages: 192-196
  • DOI: 10.1007/s13691-016-0256-8
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A comparative study of 2 screening tools for locomotive syndrome (the “Loco-check” and the “GLFS-25”): An orthopedic outpatient-based survey. (2016)
  • Author(s): Kim Y, Suehara Y, Ishii M, Kawasaki T, Matsuoka K, Okubo T, Okubo N, Tanabe Y, Akaike K, Mukaihara K, Kubota D, Maruyama Y, Saito T, Kaneko K.
  • Journal Title: British Journal of Medicine & Medical Research Volume: 17(5) Issue: 5 Pages: 1-13
  • DOI: 10.9734/bjmmr/2016/28194
  • Peer Reviewed / Open Access
• [Journal Article] A case of secondary chondrosarcoma with TP53 mutation arising from fibrous dysplasia. (2016)
  • Author(s): Tomomasa R, Akaike K, Asahina M, Toda-Ishii M, Yashiro D, Suehara Y, Takagi T, Kaneko K, Yao T, Saito T.
  • Journal Title: International journal of clinical and experimental pathology. Volume: 9(7) Pages: 7230-7236
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A case of epithelioid hemangioma of the right third rib in a 55-year-old man. (2016)
  • Author(s): Saito T, Akaike K, Tomomasa R, Suehara Y, Takagi T, Kaneko K, Yao T.
  • Journal Title: International journal of clinical and experimental pathology. Volume: 9(9) Pages: 9681-9686
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Clinicopathological characteristics of lung adenocarcinoma with EGFR compound mutations. (2019)
  • Author(s): Hayashi T, Kohsaka S, Takamochi K, Takahashi F, Suehara Y, SaitoT, Suzuki K, Mano H, Yao T.
  • Organizer: USCAP, the 2019 Annual Meeting
  • Int'l Joint Research
• [Presentation] Metabolic fluctuations in adriamycin-resistant osteosarcomas using metabolomic approaches (2019)
  • Author(s): Sasa K, Suehara Y, Akaike K, Hayashi T, Taka H, Youngji K, Okubo T, Kaneko K, Saito T.
  • Organizer: the 65th Annual Meeting of the Orthopaedic Research Society
  • Int'l Joint Research
• [Presentation] IRE1α-XBP1 Inhibitor exerts antiyumor activity in osteosarcoma. (2019)
  • Author(s): Kurihara T, Suehara Y, Hayashi T, Akaike K, Okubo T, Kim Y, Kaneko K, Saito T.
  • Organizer: the 65th Annual Meeting of the Orthopaedic Research Society
  • Int'l Joint Research
• [Presentation] Comprehensive mRNA-based screen for tyrosine kinase fusions and a de novo alternative transcription initiation site in soft tissue sarcomas. (2018)
  • Author(s): Suehara Y, Kohsaka S, Kurisaki A, Akaike K, Hayashi T, Mogushi K, Okubo T, Kim Y, Sato S, Kobayashi E, Kaneko K, Mano H, Saito T.
  • Organizer: European Society For Medical Oncology ASIA,
  • Int'l Joint Research
• [Presentation] IRE1α-XBP1 Inhibitor exerts antiyumor activity in osteosarcoma. (2018)
  • Author(s): Kurihara T, Suehara Y, Hayashi T, Akaike K, Okubo T, Kim Y, Kaneko K, Saito T.
  • Organizer: Connective Tissue Oncology Society, 23th Annual Meeting
• [Presentation] Expanded access of larotrectenib to manage pediatric TRK fusion sarcomas. (2018)
  • Author(s): Suehara Y, Trahair T, Kirby M, Saito S, Capra M, Ghosh T, Henry D, Ku NC, Cox MC, Kato S, Ziegler DS.
  • Organizer: 50th Congress of International Society of Paediatric Oncology,
• [Presentation] Clinical genomic sequencing of 72 pediatric and adult osteosarcomas reveals distinct molecular subsets with potentially targetable alterations. (2018)
  • Author(s): Alex D, Suehara Y, Bowman A, Middha S, Zehir A, Wang L, Jour G, Nafa K, Meyers P, Healey J, Hameed M, Ladanyi M.
  • Organizer: USCAP, the 2018 Annual Meeting,
  • Int'l Joint Research
• [Presentation] Anti-tumor activity of tyrosine kinase inhibitors and gene expression profiles in alveolar soft part sarcoma. (2018)
  • Author(s): Suehara Y, Tanabe Y, Mukaihara K, Kurihara K, Akaike K, Hayashi T, Mogushi K, Kaneko K, Saito T.
  • Organizer: the 64th Annual Meeting of the Orthopaedic Research Society
  • Int'l Joint Research
• [Presentation] Proteomics analysis of characteristic histological changes with Denosumab treatment in giant cell tumors of bone. (2017)
  • Author(s): SueharaY, Mukaihara K, TanabeY, Kohsaka S, Kazuno S, Ishii M, Akaike K, Kubota D, Kaneko K, Saito T.
  • Organizer: the 63th Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: San Diego, USA
  • Year and Date: 2017-03-19
  • Int'l Joint Research
• [Presentation] Proteomic approaches reveal that inhibition of the IRE1/XBP1 pathway reduces tumor growth in Ewing's sarcoma. (2017)
  • Author(s): Tanabe Y, Suehara Y, Kohsaka S, Mukaihara K, Kubota D, Akaike K, Ishii M, Kazuno S, Mineki R, Kaneko K, Ladanyi M, Saito T.
  • Organizer: the 63th Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: San Diego, USA
  • Year and Date: 2017-03-19
  • Int'l Joint Research
• [Presentation] Anti-tumor activity of tyrosine kinase inhibitors in alveolar soft part sarcoma. (2017)
  • Author(s): Suehara Y, Tanabe Y, Akaike K, Mogushi K, Hayashi T, Kurihara T, Kaneko K, Saito T.
  • Organizer: European Society For Medical Oncology ASIA
  • Int'l Joint Research
• [Presentation] A complete responder to pazopanib in high-grade soft tissue sarcomas. (2017)
  • Author(s): Suehara Y, Kohsaka S, Yamaguchi S, Akaike K, Hayashi T, Tanabe Y, Ueno T, Kojima S, Takeda I, Hayashi R, Saito T, Mano H, Kato S.
  • Organizer: Connective Tissue Oncology Society, 22th Annual Meeting
  • Int'l Joint Research
• [Presentation] Diagnostic strategy for skeletal metastasis of unknown primary origin at the initial visit/ Up-to-date treatment of skeletal metastasis (2017)
  • Author(s): Kim Y, Suehara Y, Kaneko K.
  • Organizer: 27th Korean-Japanese Combined Orthopedic Symposium
  • Int'l Joint Research / Invited
• [Presentation] Large skin ulcer due to a subcutaneous orthopedic implant after bevacizumab therapy: A case report. (2017)
  • Author(s): Suehara Y, Kubota D, Kato S, Akaike K, Tanabe Y, Okubo T, Kim Y, Saito T, Kaneko K.
  • Organizer: 19th, International Symposium on Limb Salvage
  • Int'l Joint Research
• [Presentation] Microsatellite instability analysis using the Promega panel in lung adenocarcinoma. (2016)
  • Author(s): Takamochi K, Takahashi F, Suehara Y, Kitano S, Sato E, Kohsaka S, Hayashi T, Mano H, Suzuki K.
  • Organizer: Chest Annual Meeting 2016
  • Place of Presentation: Los Angels, USA
  • Year and Date: 2016-10-22
  • Int'l Joint Research
• [Presentation] The Regulation of Malignant Transformation by PPP2R1A in Alveolar Rhabdomyosarcoma Based On A Proteomic Approach Corresponding To The PAX3/FOXO1 Fusion Gene. (2016)
  • Author(s): Akaike K, Suehara Y, Kohsaka S, Mukaihara K, Ishii M, Kubota D, Tanabe Y, Kazuno S, Mineki R, Fujimura T, Kaneko K, Ladanyi M, Saito T.
  • Organizer: Connective Tissue Oncology Society, 21th Annual Meeting,
  • Place of Presentation: Lisbon, Portugal
  • Year and Date: 2016-09-09
  • Int'l Joint Research
• [Presentation] Protein Expression Profiles Corresponding To Histological Changes With Denosumab Treatment in Giant Cell Tumors of Bone. (2016)
  • Author(s): Suehara Y, Mukaihara K, Tanabe Y, Kohsaka S, Kazuno S, Ishii M, Akaike K, Kubota D, Kaneko K, Saito T.
  • Organizer: Connective Tissue Oncology Society, 21th Annual Meeting,
  • Place of Presentation: Lisbon, Portugal
  • Year and Date: 2016-09-09
  • Int'l Joint Research
• [Presentation] Proteomic approaches revealed inhibition of IRE1/XBP1 pathway reduces tumor growths in Ewing's sarcomas. (2016)
  • Author(s): Tanabe Y, Suehara Y, Kohsaka S, Mukaihara K, Kubota D, Akaike K, Ishii M, Kazuno S, Mineki R, Kaneko K, Ladanyi M, Saito T.
  • Organizer: Connective Tissue Oncology Society, 21th Annual Meeting,
  • Place of Presentation: Lisbon, Portugal
  • Year and Date: 2016-09-09
  • Int'l Joint Research
• [Presentation] Protein expression profiling corresponding to giant cell tumors of bone treated with denosumab. (2016)
  • Author(s): Mukaihara K, Suehara Y, Akaike K, Tanabe Y, Kubota D, Ishii M, Kaneko K, Saito T.
  • Organizer: The 11th Meeting of The Asia Pacific Musculoskeletal Tumour Society (APMSTS)
  • Place of Presentation: Singapore, USA
  • Year and Date: 2016-04-21
  • Int'l Joint Research
• [Presentation] Our efforts to bone and soft tissue tumors and bone metastases at Juntendo University. (2016)
  • Author(s): Kaneko K, Suehara Y, Yonji K, Takagi T.
  • Organizer: The Korean Bone and Joint Tumor Society (KBJTS)
  • Place of Presentation: Incheon, Korea
  • Int'l Joint Research / Invited