| Project/Area Number |
16K15681
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Anesthesiology
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| Research Institution | Toho University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
山崎 創 東邦大学, 医学部, 准教授 (70315084)
中野 裕康 東邦大学, 医学部, 教授 (70276476)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKANO Hiroyasu 東邦大学, 医学部, 教授 (70276476)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | Histone / sepsis / cell death / gene trap / ヒストン / 細胞死 / 敗血症 / Gene-trap / CRISPR/Cas9 / CRISPR-Cas9 |
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| Outline of Final Research Achievements |
Circulating histones are major mediators of sepsis and cytotoxic to vascular endothelium. For analyzing cytotoxic mechanism of histones, we constructed a haploid genetic screen system using HAP1 cells. Infection efficiency of gene-trap vector to HAP1 cells was 32%, it revealed that high frequency integration was occurred in gene structures. We treated gene-trapped HAP1 cells with histones, surviving cells were treated with histones again.
We could not find a mediator gene of histone toxicity, but histones have possibilities medical targets for sepsis.
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