Differentiation of Leydig cells

• Project/Area Number: 16K15688
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Urology
• Research Institution: Kobe University
• Principal Investigator: Ishida Takaki 神戸大学, 医学部附属病院, 医員 (10771850)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: leydig細胞 / ヒトiPS細胞 / テストステロン / NR5A1 / 17βHSD3 / LHCGR / アルドステロン / コルチゾール / 分化誘導 / Leydig細胞 / 薬剤誘導性NR5A1強制発現細胞 / テストステロン産生Leydig細胞 / アンドロロジー

Outline of Final Research Achievements

we transduced NR5A1 expressing system by doxycycline addition to human iPS cell. NR5A1 forced expression iPS cells differentiate into Human iPS cells-derived testosterone-producing Leydig cells. Leydig cell marker (INSL3 variant2) and LH receptor (LHCGR) and Leydig cell-specific enzyme（17βHSD3）and expression of various enzyme (StAR,CYP11A,3βHSD2,CYP17A) necessary for testosterone production were confirmed in RT-PCR.INSL3 and LHCGR were confirmed in immunohistochemical staining. Testosterone production was confirmed by the hormone measurement in supernatants.

Research Products

• [Presentation] Differentiation of hormone-producing Leydig cells from human iPS cells (2018)
  • Author(s): 石田貴樹
  • Organizer: International Society for Sexual MedicineWorld Meeting
  • Int'l Joint Research / Invited
• [Presentation] Differentiation of hormone-producing Leydig cells from human iPS cells (2017)
  • Author(s): Takaki Ishida Mikito Tanaka Kenta Sumii Teruo Fukuda Koji Chiba Kei Matushita Yuzo Nakano Masato Fujisawa Takashi Aoi
  • Organizer: I2017CA2017
  • Place of Presentation: コペンハーゲン
  • Year and Date: 2017-05-06
  • Int'l Joint Research / Invited
• [Presentation] Differentiation of hormone-producing Leydig cells from human iPS cells (2017)
  • Author(s): Takaki Ishida Mikito Tanaka Kenta Sumii Teruo Fukuda Koji Chiba Kei Matushita Yuzo Nakano Masato Fujisawa Takashi Aoi
  • Organizer: 105回 日本泌尿器科学会総会
  • Place of Presentation: 鹿児島
  • Year and Date: 2017-04-21
• [Presentation] ヒトiPS細胞由来Leydig細胞の作製 (2017)
  • Author(s): 石田貴樹 田中幹人 角井健太 福田輝雄 江夏徳寿 千葉公嗣 松下 経 中野雄造 藤澤正人 青井貴之
  • Organizer: 日本生殖再生医学会
  • Place of Presentation: 東京
  • Year and Date: 2017-03-19
• [Presentation] ヒトiPS細胞由来Leydig細胞の作製 (2017)
  • Author(s): 石田貴樹 田中幹人 角井健太 福田輝雄 江夏徳寿 千葉公嗣 松下 経 中野雄造 藤澤正人 青井貴之
  • Organizer: 生殖内分泌学会
  • Place of Presentation: 大阪
  • Year and Date: 2017-01-14
• [Presentation] ヒトiPS細胞由来テストステロン産生Leydig細胞の作製 (2017)
  • Author(s): 石田貴樹
  • Organizer: 第62回 日本生殖医学会
• [Presentation] ヒトiPS細胞由来テストステロン産生Leydig細胞の作製 (2017)
  • Author(s): 石田貴樹
  • Organizer: 第22回 日本生殖内分泌学会
• [Presentation] ヒトiPS細胞由来テストステロン産生Leydig細胞の作製 (2017)
  • Author(s): 石田貴樹
  • Organizer: 中部泌尿器科学会総会
  • Invited
• [Patent(Industrial Property Rights)] ヒト多能性幹細胞由来ライディッヒ様細胞の作製方法及びヒト多能 性幹細胞由来ライディッヒ様細胞 (2016)
  • Inventor(s): 青井 貴之、石田 貴樹、藤澤 正人
  • Industrial Property Rights Holder: 青井 貴之、石田 貴樹、藤澤 正人
  • Industrial Property Rights Type: 特許
  • Filing Date: 2016-11-10