Differentiation of Leydig cells
| Project/Area Number |
16K15688
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Urology
|
|---|
| Research Institution | Kobe University |
|---|
Principal Investigator |
Ishida Takaki 神戸大学, 医学部附属病院, 医員 (10771850)
|
|---|
| Project Period (FY) |
2016-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | leydig細胞 / ヒトiPS細胞 / テストステロン / NR5A1 / 17βHSD3 / LHCGR / アルドステロン / コルチゾール / 分化誘導 / Leydig細胞 / 薬剤誘導性NR5A1強制発現細胞 / テストステロン産生Leydig細胞 / アンドロロジー |
|---|
| Outline of Final Research Achievements |
we transduced NR5A1 expressing system by doxycycline addition to human iPS cell. NR5A1 forced expression iPS cells differentiate into Human iPS cells-derived testosterone-producing Leydig cells. Leydig cell marker (INSL3 variant2) and LH receptor (LHCGR) and Leydig cell-specific enzyme（17βHSD3）and expression of various enzyme (StAR,CYP11A,3βHSD2,CYP17A) necessary for testosterone production were confirmed in RT-PCR.INSL3 and LHCGR were confirmed in immunohistochemical staining. Testosterone production was confirmed by the hormone measurement in supernatants.
|
Report
(3 results)
Research Products
(9 results)
