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Differentiation of Leydig cells

Research Project

Project/Area Number 16K15688
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Urology
Research InstitutionKobe University

Principal Investigator

Ishida Takaki  神戸大学, 医学部附属病院, 医員 (10771850)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsleydig細胞 / ヒトiPS細胞 / テストステロン / NR5A1 / 17βHSD3 / LHCGR / アルドステロン / コルチゾール / 分化誘導 / Leydig細胞 / 薬剤誘導性NR5A1強制発現細胞 / テストステロン産生Leydig細胞 / アンドロロジー
Outline of Final Research Achievements

we transduced NR5A1 expressing system by doxycycline addition to human iPS cell. NR5A1 forced expression iPS cells differentiate into Human iPS cells-derived testosterone-producing Leydig cells. Leydig cell marker (INSL3 variant2) and LH receptor (LHCGR) and Leydig cell-specific enzyme(17βHSD3)and expression of various enzyme (StAR,CYP11A,3βHSD2,CYP17A) necessary for testosterone production were confirmed in RT-PCR.INSL3 and LHCGR were confirmed in immunohistochemical staining. Testosterone production was confirmed by the hormone measurement in supernatants.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (9 results)

All 2018 2017 2016

All Presentation (8 results) (of which Int'l Joint Research: 2 results,  Invited: 3 results) Patent(Industrial Property Rights) (1 results)

  • [Presentation] Differentiation of hormone-producing Leydig cells from human iPS cells2018

    • Author(s)
      石田貴樹
    • Organizer
      International Society for Sexual MedicineWorld Meeting
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Differentiation of hormone-producing Leydig cells from human iPS cells2017

    • Author(s)
      Takaki Ishida Mikito Tanaka Kenta Sumii Teruo Fukuda Koji Chiba Kei Matushita Yuzo Nakano Masato Fujisawa Takashi Aoi
    • Organizer
      I2017CA2017
    • Place of Presentation
      コペンハーゲン
    • Year and Date
      2017-05-06
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Differentiation of hormone-producing Leydig cells from human iPS cells2017

    • Author(s)
      Takaki Ishida Mikito Tanaka Kenta Sumii Teruo Fukuda Koji Chiba Kei Matushita Yuzo Nakano Masato Fujisawa Takashi Aoi
    • Organizer
      105回 日本泌尿器科学会総会
    • Place of Presentation
      鹿児島
    • Year and Date
      2017-04-21
    • Related Report
      2016 Research-status Report
  • [Presentation] ヒトiPS細胞由来Leydig細胞の作製2017

    • Author(s)
      石田貴樹 田中幹人 角井健太 福田輝雄  江夏徳寿  千葉公嗣  松下 経  中野雄造  藤澤正人  青井貴之
    • Organizer
      日本生殖再生医学会
    • Place of Presentation
      東京
    • Year and Date
      2017-03-19
    • Related Report
      2016 Research-status Report
  • [Presentation] ヒトiPS細胞由来Leydig細胞の作製2017

    • Author(s)
      石田貴樹  田中幹人 角井健太 福田輝雄  江夏徳寿  千葉公嗣  松下 経  中野雄造  藤澤正人  青井貴之
    • Organizer
      生殖内分泌学会
    • Place of Presentation
      大阪
    • Year and Date
      2017-01-14
    • Related Report
      2016 Research-status Report
  • [Presentation] ヒトiPS細胞由来テストステロン産生Leydig細胞の作製2017

    • Author(s)
      石田貴樹
    • Organizer
      第62回 日本生殖医学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ヒトiPS細胞由来テストステロン産生Leydig細胞の作製2017

    • Author(s)
      石田貴樹
    • Organizer
      第22回 日本生殖内分泌学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ヒトiPS細胞由来テストステロン産生Leydig細胞の作製2017

    • Author(s)
      石田貴樹
    • Organizer
      中部泌尿器科学会総会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Patent(Industrial Property Rights)] ヒト多能性幹細胞由来ライディッヒ様細胞の作製方法及びヒト多能 性幹細胞由来ライディッヒ様細胞2016

    • Inventor(s)
      青井 貴之、石田 貴樹、藤澤 正人
    • Industrial Property Rights Holder
      青井 貴之、石田 貴樹、藤澤 正人
    • Industrial Property Rights Type
      特許
    • Filing Date
      2016-11-10
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2022-12-28  

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