Hypersensitive diagnosis of urothelial cancer using silver nanoscale hexagonal column

• Project/Area Number: 16K15696
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Urology
• Research Institution: Osaka International Cancer Institute
• Principal Investigator: Nishimura Kazuo 地方独立行政法人大阪府立病院機構大阪国際がんセンター(研究所), その他部局等, 泌尿器科主任部長 (80303957)
• Co-Investigator(Kenkyū-buntansha): 中山 雅志 地方独立行政法人大阪府立病院機構大阪国際がんセンター(研究所), その他部局等, その他 (40379178)
• Co-Investigator(Renkei-kenkyūsha): Maeda Kazuhisa 前田クリニック, 院長 (60397750)
• Research Collaborator: Hasegawa Katsuyuki 有限会社マイテック
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ヌクレオソーム / 銀ナノ錯体 / 尿路上皮癌 / 臨床

Outline of Final Research Achievements

The aim of this study is to analyze circulating nucleosome using silver nanoscale hexagonal columns (NHCs) for the purpose of early diagnosis and staging of urothelial cancer (renal pelvic, ureteral and bladder cancer). Circulating nucleosomes in serum formed crystals on a silver NHC chip. Using serum obtained from urothelial cancer patients and patients with benign disease, the crystals on a silver NHC chip were observed under fluorescence microscope. The size and the number of the crystals obtained from urothelial cancer tended to be larger and higher. But neither the size nor the number of the crystals has a significant association with cancer stage.
Next, spectroscopic analysis was performed using crystals from serum samples on silver NHC chips. There were different peak wave lengths between urothelial cancer patients and patients with benign disease. The spectroscopic analysis of the crystals on a silver NHC chip may distinguish urothelial cancer from benign disease.