Analysis of chorionic villi-derived embryo stem cells by novel sten cell markers, laeberin and hTERT

• Project/Area Number: 16K15702
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: Kanazawa University
• Principal Investigator: Hiroshi Fujiwara 金沢大学, 医学系, 教授 (30252456)
• Co-Investigator(Kenkyū-buntansha): 高倉 正博 金沢大学, 附属病院, 准教授 (20313661) ; 大黒 多希子 金沢大学, 学際科学実験センター, 教授 (30767249) ; 服部 明 京都大学, 薬学研究科, 准教授 (50300893) ; 河崎 洋志 金沢大学, 脳・肝インターフェースメディシン研究センター, 教授 (50303904) ; 荒木 慶彦 順天堂大学, 医学(系)研究科(研究院), 先任准教授 (70250933)
• Project Period (FY): 2016-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2016: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
• Keywords: laeverin / 臍帯血 / 初期絨毛 / hTERT / Oct4 / テロメラーゼ活性 / 胎児循環幹細胞 / ヒト絨毛

Outline of Final Research Achievements

By this project, laeverin-positive cells were successfully isolated from umbilical cord blood and chorionic villous tissues. Consequently, we have become able to analyze their gene and protein expression profiles. This study also demonstrated the precise localization of laeverin-positive cells in the hunan chorionic villous tissues in the early pregnancy. We hope that these findings will contribute to analyzing cell differentiation mechanisms in the field of regenerative or reproductive medicine.

Research Products

• [Journal Article] Platelet-derived microparticles and soluble factors differentially regulate human endometrial epithelial cell movement (2017)
  • Author(s): Suginami K, Sato Y, Horie A, Matsumoto H, Tani H, Mizumoto Y, Ono M, Matsuoka A, Kyo S, Araki Y, Konishi I, Fujiwara H
  • Journal Title: Am J Reprod Immunol Volume: Feb 7 Issue: 4 Pages: 12641-12641
  • DOI: 10.1111/aji.12641
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Acquired hemophilia A manifesting as plasma transfusion-uncontrolled severe bleeding 2 weeks after chorioamnionitis-induced abortion (2016)
  • Author(s): Matsuoka A, Sasaki H, Sugimori C, Hirabuki S, Hoshiba T, Fujiwara H.
  • Journal Title: J Obstet Gynaecol Res Volume: Feb 2 Issue: 4 Pages: 13270-13270
  • DOI: 10.1111/jog.13270
  • Peer Reviewed
• [Journal Article] FDG-PET-positive ovarian thecoma with GLUT5 expression: Five cases (2016)
  • Author(s): Bono Y, Mizumoto Y, Nakamura M, Iwadare J, Obata T, Fujiwara H
  • Journal Title: J Obstet Gynaecol Res Volume: 43 Issue: 3 Pages: 599-603
  • DOI: 10.1111/jog.13243
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Platelets are a possible regulator of human endometrial re-epithelialization during menstruation (2016)
  • Author(s): Suginami K, Sato Y, Horie A, Matsumoto H, Kyo S, Araki Y, Konishi I, Fujiwara H
  • Journal Title: Am J Reprod Immunol Volume: 77 Issue: 1 Pages: 12609-12609
  • DOI: 10.1111/aji.12609
  • Peer Reviewed
• [Journal Article] CD9 suppresses human extravillous trophoblast invasion (2016)
  • Author(s): Matsumoto H, Sato Y, Horie A, Suginami K, Tani H, Hattori A, Araki Y, Kagami K, Konishi I, Fujiwara H
  • Journal Title: Placenta Volume: 47 Pages: 105-112
  • DOI: 10.1016/j.placenta.2016.09.014
  • Peer Reviewed
• [Journal Article] Synchronous endometrioid adenocarcinomas in the uterine cervix and corpus (2016)
  • Author(s): Obata T, Nakamura M, Mizumoto Y, Matsumoto T, Takakura M, Fujiwara H
  • Journal Title: J Obstet Gynaecol Res Volume: 42 Issue: 10 Pages: 1390-1394
  • DOI: 10.1111/jog.13049
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Identification of an anti-sperm auto-monoclonal antibody (Ts4)-recognized molecule in the mouse sperm acrosomal region and its inhibitory effects on fertilization in vitro. (2016)
  • Author(s): Yoshitake H., Oda R., Yanagida M., Kawasaki Y., Sakuraba M., Takamori K., Hasegawa A., Fujiwara H., Araki Y.
  • Journal Title: J.Reprod.Immunol. Volume: 115 Pages: 6-13
  • DOI: 10.1016/j.jri.2016.04.001
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Familial discrepancy of clinical outcomes associated with fibrinogen Dorfen: A case of huge genital hematoma after episiotomy (2016)
  • Author(s): Kagami K, Yamazaki R, Minami T, Okumura N, Morishita E, Fujiwara H
  • Journal Title: J Obstet Gynaecol Res Volume: 42 Issue: 6 Pages: 722-725
  • DOI: 10.1111/jog.12972
  • Peer Reviewed / Open Access / Int'l Joint Research