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In vivo functional genomics screen reveals mechanism of peritoneal dissemination in ovarian cancer

Research Project

Project/Area Number 16K15706
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Obstetrics and gynecology
Research InstitutionKyoto University

Principal Investigator

Konishi Ikuo  京都大学, 医学研究科, 名誉教授 (90192062)

Co-Investigator(Kenkyū-buntansha) 松村 謙臣  近畿大学, 医学部, 教授 (20452336)
安彦 郁  京都大学, 医学(系)研究科(研究院), 助教 (20508246)
濱西 潤三  京都大学, 医学研究科, 講師 (80378736)
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords卵巣癌 / 癌幹細胞 / 腹膜播種 / 機能ゲノミクススクリーニング
Outline of Final Research Achievements

A library of 81 000 shRNAs targeting 15 000 human genes was stably transfected to ovarian cancer cell lines. By functional screening of these cancer cells, we screened genes that are responsible for anoikis resistance when down-regulated. One of the screened genes was ABHD2, which was fuctionally related to cisplatin resistance. Furthermore, we identified genes that increase Side Population cells when down-regulated. Side Population cells are defined as cells which have ability to efflux Hoechst 33342. Down-regulation of these genes were related to cancer stemness phenotype.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (4 results)

All 2017 2016

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (3 results)

  • [Journal Article] Suppression of ABHD2, identified through a functional genomics screen, causes anoikis resistance, chemoresistance and poor prognosis in ovarian cancer.2016

    • Author(s)
      Yamanoi K, Matsumura N, Murphy SK, Baba T, Abiko K, Hamanishi J, Yamaguchi K, Koshiyama M, Konishi I, Mandai M.
    • Journal Title

      Oncotarget.

      Volume: Jul 26;7(30) Issue: 30 Pages: 47620-47636

    • DOI

      10.18632/oncotarget.9951

    • NAID

      120005820858

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 機能的ゲノミクススクリーンによる、卵巣癌のside population分画を制御する因子の探索2017

    • Author(s)
      山ノ井康二
    • Organizer
      生殖医学フォーラム
    • Related Report
      2017 Annual Research Report
  • [Presentation] 機能的ゲノミクススクリーニングによる、卵巣がんのside population (SP)分画を制御する6遺伝子の同定2016

    • Author(s)
      山ノ井康二、松村謙臣、安彦郁、山口建、濱西潤三、馬場長、越山雅文、小西郁生
    • Organizer
      第75回日本癌学会総会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2016-10-06
    • Related Report
      2016 Research-status Report
  • [Presentation] Identification through a functional genomics screen of factors whose down-regulation enhances the cancer stem cell population in ovarian cancer2016

    • Author(s)
      Koji Yamanoi, Noriomi Matsumura, Kaoru Abiko, Ken Yamaguchi, Junzo Hamanishi, Tsukasa Baba, Masafumi Koshiyama, Ikuo Konishi
    • Organizer
      第68回日本産科婦人科学会学術講演会
    • Place of Presentation
      東京国際フォーラム(東京都千代田区)
    • Year and Date
      2016-04-21
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2019-03-29  

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