| Project/Area Number |
16K15712
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | St. Marianna University School of Medicine |
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Principal Investigator |
OHTA Tomohiko 聖マリアンナ医科大学, 医学研究科, 教授 (60233136)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | BRCA1 / ERα / 染色体不安定性 / ヘテロクロマチン / アポトーシス / Chk2 / 臓器特異性 / 膵癌 |
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| Outline of Final Research Achievements |
MCF10A cells stably expressing ERα together with BRCA1-spedific shRNA in a doxycycline-inducible manner were investigated to analyze mechanisms underlying ERα-induced genomic instability in BRCA1 deficient background. ERα expressing BRCA1-depleted cells exhibited flattened morphology, increased multiple nuclei with micronuclei, and tended to display increased mitotic chromosomal bridges, whereas they did not show significant difference in secondary DNA structures such as R-loop and G-quadruplex when compared to control cells.
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