Insufficiency of a novel anti-oncogene and mouse ovarian tumorigenesis

• Project/Area Number: 16K15714
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: Miyagi Prefectural Hospital Organization Miyagi Cancer Center
• Principal Investigator: YAMADA Hidekazu 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所), がん薬物療法研究部, 特任研究員 (10254012)
• Co-Investigator(Kenkyū-buntansha): 島 礼 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所), がん薬物療法研究部, 部長 (10196462) ; 伊藤 しげみ 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所), がん薬物療法研究部, 特任研究員 (80600006)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 卵巣がん / プロテインホスファターゼ / 婦人科腫瘍学

Outline of Final Research Achievements

In order to address the function of protein phosphatase 6 (PP6) loss on K-ras-initiated tumorigenesis in ovary. To do so, we developed tamoxifen-inducible K-rasG12D-expressing mice and double mutant (K-rasG12D-expressing and Ppp6c-deficient) mice in which K-rasG12D expression is driven by the cytokeratin 14 (K14) promoter. Using 8week old and 12 weeks old female mice, intrabursal injection of an adenovirus-Cre construct was conducted. Eight month after the induction, mice were dissected and ovary was examined. Both K-rasG12D-expressing mice and doubly-mutant mice showed no tumors in the ovary. The results showed that K-rasG12D expression using this system is not sufficient to develop ovarian tumors in mice.

Research Products

• [Journal Article] Loss of protein phosphatase 6 in mouse keratinocytes enhances K-rasG12D-driven tumor promotion. (2018)
  • Author(s): Kurosawa K, Inoue Y, Kakugawa Y, Yamashita Y, Kanazawa K, Kishimoto K, Nomura M, Momoi Y, Sato I, Chiba N, Suzuki M, Ogoh H, Yamada H, Miura K, Watanabe T, Tanuma N, Tachi M, and Shima H
  • Journal Title: Cancer Science Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Current Status of Uterine Leiomyosarcoma in the Tohoku Region: Results of the Tohoku Translational Center Development Network Survey (2017)
  • Author(s): Tokunaga H, Takahashi F, Yamamoto H, Honda T, Watanabe T, Shoji T, Sugiyama T, Yamada H, Tando T, Yoshinaga K, Kagabu S, Otsuki T, Kin S, Yokoyama Y, Wagatsuma S, Sato K, Sato H, Oishi T, Yoshida Y, Hayasaka T, Matsui T, Imai N, Nishigori H, Shimokawa H, Yaegashi N, Watanabe Y.
  • Journal Title: International Journal of Clinical Oncology Volume: 22 Issue: 3 Pages: 541-547
  • DOI: 10.1007/s10147-017-1097-y
  • Peer Reviewed
• [Journal Article] Clinical efficacy of nedaplatin based concurrent chemoradiotherapy for uterine cervical cancer (2016)
  • Author(s): Kagabu M, Shoji T , Murakami K, Omi H, Honda T, Miura F, Yokoyama Y, Tokunaga H,Takano T, Ohta T , Shimizu D & Sato N, Soeda S , Watanabe T, Yamada H, Mizunuma H, Yaegashi N Nagase S,Tase T, Sugiyama T
  • Journal Title: a Tohoku Gynecologic Cancer Unit Study. Int J Clin Oncol Volume: 21（4） Issue: 4 Pages: 735-740
  • DOI: 10.1007/s10147-016-0946-4
  • Peer Reviewed
• [Presentation] 放射線治療後に局所に病巣が残存した子宮頸癌に対する子宮全摘は有効か？ (2016)
  • Author(s): 山田秀和、藤田信弘、大友圭子、田勢 亨
  • Organizer: 第３９回日本産婦人科手術学会
  • Place of Presentation: 宮城県・仙台市
  • Year and Date: 2016-11-12