Development of whole labyrinth imaging by bone tissue trasnparency
| Project/Area Number |
16K15717
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Otorhinolaryngology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
OKABE Shigeo 東京大学, 医学部, 教授 (60204012)
MATSUMOTO Yu 東京大学, 医学部附属病院, 特任講師 (80548553)
FUJIMOTO Chisato 東京大学, 医学部附属病院, 助教 (60581882)
KASHIO Akinori 東京大学, 医学部附属病院, 助教 (20451809)
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| Research Collaborator |
URATA Shinji 東京大学, 大学院医学系研究科, 大学院生
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 神経科学 / 脳・神経 / 解剖学 / 蝸牛 / 内耳 / 細胞・組織 |
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| Outline of Final Research Achievements |
We successfully modified a sorbitol-based optical clearing method, ScaleS,for use in high resolution imaging of the entire cochlea, an essential hearing organ within the temporal bone. We reported a tissue clearing-based analytical pipeline for the construction of a single-cellular resolution imaging of the entire cochlea.This method can be completed within two days for the fluorescent protein detection or within three days for immunohistochemistry of endogenous proteins. We generated entire cochlear maps at different postnatal ages with or without noise-induced cochlear damage by recording the cell distribution along the entire longitudinal axis. We next reported machine learning-based pattern recognition automated high-fidelity detection and analysis of total hair cell positions along the longitudinal axis of the organ of Corti.Our method of cellular mapping is highly effective in system-level phenotyping of the organ of Corti under both physiological and pathological conditions.
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Report
(3 results)
Research Products
(5 results)
