Establishment tailor-made therapy of head and neck cancer by single cell analysis

• Project/Area Number: 16K15718
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Otorhinolaryngology
• Research Institution: Kanazawa University
• Principal Investigator: Satoru Kondo 金沢大学, 附属病院, 講師 (70436822)
• Co-Investigator(Kenkyū-buntansha): 吉崎 智一 金沢大学, 医学系, 教授 (70262582)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: シングルセル / 血中循環腫瘍細胞 / EpCAM / 末梢循環癌細胞 / 次世代シークエンス

Outline of Final Research Achievements

Cancer tissues have various heterogeneity and very few cells indicate its phenotypes of cancer. This variety leads difficulty of assessment of cancer phenotypes by ordinal methods such as PET, miRNA and microarray. Exact assessment of cancer phenotypes is necessary to determine groups which has highly malignant cancer cells. From this aspect, we focused circulating tumor cell (CTC). Because CTC has capacity of high invasion and metastasis. So CTC is considered to reflect cancer grades and phenotypes. In this study, we focused CTC and analyzed detection of CTC.

Research Products

• [Journal Article] Expression of secreted protein acidic and rich in cysteine is an independent prognostic indicator of a poor clinical outcome in oropharyngeal carcinoma patients (2016)
  • Author(s): Yoshida S, Wakisaka N, Kondo S, Moriyama-Kita M, Hirai N, Endo K, Tsuji A, Nakanishi Y, Murono S, Yoshizaki T
  • Journal Title: Acta Otolaryngol Volume: 136 Issue: 2 Pages: 189-94
  • DOI: 10.3109/00016489.2015.1103381
  • NAID: 120005851371
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] T-status and an oral fluoropyrimidine, S-1, adjuvant chemotherapy are prognostic factors in reduced-RADPLAT for resectable hypopharyngeal cancer (2016)
  • Author(s): Wakisaka N, Hirai N, Kondo S, Aga M, Nakanishi Y, Tsuji A, Endo K, Murono S, Yoshizaki T
  • Journal Title: Acta Otolaryngol Volume: 136 Issue: 8 Pages: 834-840
  • DOI: 10.3109/00016489.2016.1157728
  • NAID: 120005774897
  • Peer Reviewed / Open Access
• [Presentation] 上咽頭癌と免疫療法 (2017)
  • Author(s): 近藤悟, 吉崎智一
  • Organizer: 第35回日本耳鼻咽喉科免疫アレルギー学会
  • Invited