Bioengineering of transplantable small intestine using decellularized intestinal scaffold

• Project/Area Number: 16K15742
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Pediatric surgery
• Research Institution: Kyoto University
• Principal Investigator: Okamoto Shinya 京都大学, 医学研究科, 客員研究員 (00329377)
• Co-Investigator(Kenkyū-buntansha): 安近 健太郎 京都大学, 医学研究科, 客員研究員 (00378895) ; 上本 伸二 京都大学, 医学研究科, 教授 (40252449) ; 石井 隆道 京都大学, 再生医科学研究所, 研究員 (70456789)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 小腸移植 / 小腸再生 / 脱細胞化小腸 / 再生医学 / 人工小腸

Outline of Final Research Achievements

Although small bowel transplantation is the only curative treatment for patients with irreversible intestinal failure, graft failure rate remains high. Therefore, a new treatment strategy for intestinal failure is required. The aim of this study is to fabricate a transplantable and functional small intestine using 3-dimensional decellularized intestinal scaffold. After removal of the small intestine from Lewis rat, the decellularized small intestinal scaffold was prepared by perfusing detergent solution via the vessel. We implanted the decellularized small intestinal scaffold to rat, and histological analysis was performed. Infiltration of intestinal mucosal epithelium and smooth muscle cells in addition to angiogenesis into the decellularized intestinal scaffold could be observed. It was suggested that the decellularized small intestinal scaffold might promote regeneration of the small bowel with infiltration of small intestinal components from recipient.

Research Products

• [Presentation] 脱細胞化小腸グラフトを使用した、生体内での小腸再生の試み (2018)
  • Author(s): 小島秀信、石井隆道、福光 剣、大島 侑、 川本浩史、南 貴人、宮内雄也、山岡竜也、 河合隆之、岡本晋弥、上本伸二
  • Organizer: 第30回日本小腸移植研究会
• [Presentation] Liver sinusoidal endothelial cell as a cell source to fabricate vasculature and maintain performance of hepatocyte in engineered liver graft (2016)
  • Author(s): Hidenobu Kojima, Kentaro Yasuchika, Ken Fukumitsu, Satoshi Ogiso, Yuya Miyauchi, Ryoya Yamaoka, Takayuki Kawai, Hokahiro Katayama, Elena Y Yoshitoshi, Sadahiko Kita, Katsutaro Yasuda, Naoya Sasaki, Takamichi Ishii, Junji Komori, Shinji Uemoto
  • Organizer: The Liver Meeting 2016. American Association for the Study of Liver Disease
  • Place of Presentation: Boston, MA, USA
  • Year and Date: 2016-11-11
  • Int'l Joint Research
• [Presentation] Optimizing recellularization of decellularized whole-liver graft: from which route and with which cell? (2016)
  • Author(s): Satoshi Ogiso, Kentaro Yasuchika, Ken Fukumitsu, Takamichi Ishii, Hidenobu Kojima, Yuya Miyauchi, Ryoya Yamaoka, Hokahiro Katayama, Takayuki Kawai, Sadahiko Kita, Shinji Uemoto
  • Organizer: the American College of Surgeons 102nd Clinical Congress
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2016-10-19
  • Int'l Joint Research
• [Presentation] Biliary tree as a window for repopulation of the decellularized liver scaffold. (2016)
  • Author(s): Satoshi Ogiso, Kentaro Yasuchika, Ken Fukumitsu, Takamichi Ishii, Hidenobu Kojima, Yuya Miyauchi, Ryoya Yamaoka, Hokahiro Katayama, Takayuki Kawai, Elena Yukie Yoshitoshi, Sadahiko Kita, Katsutaro Yasuda, Shinji Uemoto
  • Organizer: 26th International congress of the transplantation society
  • Place of Presentation: Hong Kong
  • Year and Date: 2016-08-20
  • Int'l Joint Research
• [Presentation] 高機能立体肝臓の構築を目指した脱細胞化肝臓に対する再細胞化アプローチの検討 (2016)
  • Author(s): 小木曾 聡、安近健太郎、福光 剣、小島秀信、宮内雄也、山岡竜也、片山外大、河合隆之、吉利エレーナ、喜多貞彦、安田勝太郎、上本伸二
  • Organizer: 第116回日本外科学会定期学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2016-04-16