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Identification of the enteric neural crest-derived cell migration-regulated genes

Research Project

Project/Area Number 16K15743
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Pediatric surgery
Research InstitutionKanazawa Medical University

Principal Investigator

KOHNO Miyuki  金沢医科大学, 医学部, 教授 (10153496)

Co-Investigator(Renkei-kenkyūsha) YOSHIZAKI Hisayoshi  金沢医科大学, 医学部, 助教 (00443490)
YASUI Yoshitomo  金沢医科大学, 医学部, 助教 (10595325)
NISHIDA Shoichi  金沢医科大学, 医学部, 研究医 (50747251)
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords腸管神経系 / 腸管神経発生 / 小児消化器疾患 / シグナル伝達 / 発生・分化
Outline of Final Research Achievements

The enteric nervous system (ENS) is a network of neurons and glia, which are derived from enteric neural crest cells (ENCCs), and is essential for regulating peristaltic activity of the colon. ENCCs migrate along the gastrointestinal tract to form the ENS. We analyzed differential gene expression between developing mouse intestine at embroyonic days 11.5 and 15.5 in order to elucidate the mechanisms associated with variations in ENCC motility during this period. Our findings indicated significant differences in the expression of genes encoding extracellular matrix proteins during these developmental periods, as well as upregulated collagen VI (ColVI) and downregulated fibronectin (FN) in the E15.5 intestine. Furthermore, ENCC-motility assays in the presence of FN, ColVI, or a combination of the two revealed that ColVI attenuated FN-induced ENCC motility and reduced expression and phosphorylation of molecules associated with focal adhesion complexes.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (3 results)

All 2018 2017

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (1 results)

  • [Journal Article] Collagen VI suppresses fibronectin-induced enteric neural crest cell migration by downregulation of focal adhesion proteins2018

    • Author(s)
      Nishida Shoichi、Yoshizaki Hisayoshi、Yasui Yoshitomo、Kuwahara Tsuyoshi、Kiyokawa Etsuko、Kohno Miyuki
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 495 Issue: 1 Pages: 1461-1467

    • DOI

      10.1016/j.bbrc.2017.11.184

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Surgical approach for fecal incontinence with a patulous anus after transanal pull-through for Hirschsprung disease2017

    • Author(s)
      Yasui Y, Nishida S, Shironomae T, Satomi M, Kuwahara T, Kohno M
    • Journal Title

      J Pediatr Surg

      Volume: 印刷中 Issue: 6 Pages: 1070-1075

    • DOI

      10.1016/j.jpedsurg.2017.02.004

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] VI型コラーゲンの腸管神経堤由来細胞移動に及ぼす影響2017

    • Author(s)
      吉崎尚良, 西田翔一, 安井良僚, 桑原強, 清川悦子, 河野美幸
    • Organizer
      2017年度生命科学系学会合同年次大会・第40回日本分子生物学会年会・第90回日本生化学会大会
    • Related Report
      2017 Annual Research Report

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Published: 2016-04-21   Modified: 2019-03-29  

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