| Project/Area Number |
16K15744
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plastic surgery
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| Research Institution | Tohoku University |
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Principal Investigator |
Kei Yoshikawa 東北大学, 医学系研究科, 非常勤講師 (20770978)
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| Co-Investigator(Kenkyū-buntansha) |
菅野 恵美 東北大学, 医学系研究科, 講師 (10431595)
丹野 寛大 東北大学, 医学系研究科, 助教 (10755664)
館 正弘 東北大学, 医学系研究科, 教授 (50312004)
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| Co-Investigator(Renkei-kenkyūsha) |
KAWAKAMI Kazuyoshi 東北大学, 医学系研究科, 教授 (10253973)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | C型レクチン受容体 / 創傷治癒 / 炎症反応 / 創傷治癒学 / ダメージ関連分子パターン / ダメージ関連分子パターン(DAMPs) |
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| Outline of Final Research Achievements |
Macrophage inducible C-type lectin (Mincle) is an activating receptor, expressed in macrophages and dendritic cells, which senses PAMPs and DAMPs. However, the role of Mincle at the wound sites has not been elucidated. In the present study, we addressed a question how defect of Mincle affected to the healing process of wounds.
Wounds were created on the backs of wild type (WT) C57BL/6 and Mincle-deficient (KO) mice. We analyzed percent wound closure, height of granulation tissue, expression of Mincle by real-time PCR and immunohistochemistry.
The percent wound closure was significantly increased in Mincle KO mice compared to WT mice, which was associated with the increased height of granulation tissues. Mincle was detected at the early phase after wound creation. These results suggest that Mincle may be involved in the regulation of granulation at the wounded tissues, which may lead to delayed wound healing.
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