Identification of novel specific makers of neural crest-derived cells in adult and their application to bone regenerative medicine
Project/Area Number |
16K15782
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Functional basic dentistry
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Research Institution | Showa University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
山田 篤 昭和大学, 歯学部, 講師 (50407558)
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Project Period (FY) |
2016-04-01 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | 神経堤由来細胞 / 骨再生 / 毛包 / 神経堤細胞 / 成体 / 細胞表面マーカー / P0-Cre / Wnt1-Cre |
Outline of Final Research Achievements |
Neural crest-derived cells are expected to be new cell sources for regenerative medicine. They maintain stem cell properties and have multipotency to osteoblasts, chondrocytes and others. In this study, we used two lines of transgenic mice in which neural crest-derived cells were labeled with GFP to clarify cell surface molecules. Furthermore, differentiation of osteoblast-like cells and formation of bone-like tissue from neural crest-derived cells were confirmed in this study.
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Academic Significance and Societal Importance of the Research Achievements |
成長後も体内に存在し、一部の細胞で幹細胞の性質を維持する神経堤由来細胞を効率良く純化・精製する方法は、組織再生医療への応用にとって多大な影響を与えるのは確実で、効率良く採取するための方法論の確立、および硬組織再生医療の開発は臨床応用に向けた生命科学や医学の研究を牽引する重要な研究であると考える。
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Report
(4 results)
Research Products
(98 results)
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[Journal Article] Production of 8-nitro-cGMP in osteocytic cells and its upregulation by parathyroid hormone and prostaglandin E2.2019
Author(s)
Nagayama K, Miyamoto Y, Kaneko K, Yoshimura K, Sasa K, Akaike T, Fujii S, Izumida E, Uyama R, Chikazu D, Maki K, Kamijo R.
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Journal Title
In Vitro Cell Dev Biol Anim
Volume: 55
Issue: 1
Pages: 45-51
DOI
Related Report
Peer Reviewed
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[Journal Article] Monocarboxylate transporter-1 promotes osteoblast differentiation via suppression of p53, a negative regulator of osteoblast differentiation.2018
Author(s)
Sasa K, Yoshimura K, Yamada A, Suzuki D, Miyamoto Y, Imai H, Nagayama K, Maki K, Yamamoto M, Kamijo R
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Journal Title
Sci. Rep.
Volume: 8(1)
Issue: 1
Pages: 10579-10579
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Cooperation of Rho family proteins Rac1 and Cdc42 in cartilage development and calcified tissue formation2018
Author(s)
Ikehata M, Yamada A, Fujita K, Yoshida Y, Kato T, Sakashita A, Ogata H, Iijima T, Kuroda M, Chikazu D, Kamijo R.
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Journal Title
Biochem Biophys Res Commun
Volume: 印刷中
Issue: 3
Pages: 525-529
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] A preliminary report on dental implant condition among dependent elderly based on the survey among Japanese dental practitioners2018
Author(s)
Sato Y, Koyama S, Ohkubo C, Ogura S, Kamijo R, Sato S, Aida J, Izumi Y, Atsumi M, Isobe A, Baba S, Ikumi N, Watanabe F
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Journal Title
Int J Implant Dent
Volume: 4(1)
Issue: 1
Pages: 14-14
DOI
Related Report
Peer Reviewed
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[Journal Article] Comprehensive genetic exploration of selective tooth agenesis of mandibular incisors by exome sequencing2017
Author(s)
Yamaguchi T, Hosomichi K, Yano K, Kim Y, Nakaoka H, Kimura R, Otsuka H, Nonaka N, Haga S, Takahashi M, Shirota T, Kikkawa Y, Yamada A, Kamijo R, Park SB, Nakamura M, Maki K, Inoue I
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Journal Title
Hum Genome Variation
Volume: 印刷中
Issue: 1
Pages: 17005-17005
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Expression of nephronectin is enhanced by α,25-dihydroxyvitamin D3.2016
Author(s)
Hiranuma K, Yamada A, Kurosawa T, Aizawa R, Suzuki D, Saito Y, Nagahama R, Ikehata M, Tsukasaki M, Morimura N, Chikazu D, Maki K, Shirota T, Takami M, Yamamoto M, Iijima T, Kamijo R
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Journal Title
FEBS Open Bio
Volume: 6
Issue: 9
Pages: 914-918
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Degradation of bradykinin by a metalloendopeptidase from Streptococcus pyogenes.2016
Author(s)
Miyamoto, T., Akaike, T., Kawabata, S., Akuta, T., Taruki, C., Yoshitake, J., Hamada, S., Ota, F., Ikarashi, H., Yoshimura, K., Kamijo, R., Maeda, H.
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Journal Title
Journal of Oral Biosciences
Volume: 58
Issue: 4
Pages: 167-172
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] ヒト歯根膜由来細胞株の確立 Down症候群歯根膜由来細胞とのSDF-1発現解析2017
Author(s)
浅川 剛吉, 宮本 洋一, 吉村 健太郎, 笹 清人, 長谷川 智一, 馬目 瑶子, 上條 竜太郎, 杉山 智美, 帖佐 直幸, 石崎 明, 島田 幸恵
Organizer
日本歯科保存学会 2017 年度春季学術大会(第 146 回)
Related Report
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[Presentation] Establish of expression on differentiation performed periodontal ligament cells derived from human teeth - Comparative analysis that SDF-1alpha regulation of expression on periodontal ligament cells derived from Down syndrome teeth2017
Author(s)
Asakawa T, Miyamoto Y, Yoshimura K, Sasa K, Manome Y, Kamijo R, Hasegawa T, Chosa N, Ishizaki A, Oikawa M, Sugiyama T, Shiga M, Shimada Y
Organizer
The 65th Annual Meeting of Japanese Association for Dental Research
Related Report
Int'l Joint Research
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[Presentation] Genomic analysis of selective tooth agenesis of mandibular incisors2017
Author(s)
Yamaguchi T, Takahashi M, Kimura R, Otsuka H, Nonaka N, Haga S, Shirota T, Yamada A, Kamijo R, Nakamura M, Maki K, Inoue I
Organizer
The 65th Annual Meeting of Japanese Association for Dental Research
Related Report
Int'l Joint Research
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[Presentation] R848, a TLR7 ligand, inhibits invasions of B16F10 malignant melanoma in bone2017
Author(s)
Manome Y, Suzuki D, Mochizuki A, Saito E, Sasa K, Yoshimura K, Inoue T, Takami M, Inagaki K, Funatsu T, Kamijo R
Organizer
The 65th Annual Meeting of Japanese Association for Dental Research
Related Report
Int'l Joint Research
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[Presentation] 8-Nitro-cGMP promotes elongation of long bones in mice2016
Author(s)
Hoshino M, Kaneko K, Miyamoto Y, Akaike T, Yoshimura K, Suzuki D, Nagayama K, Maki K, Chikazu D, Baba K, Kamijo R
Organizer
The 9th International Conference on the Biology, Chemistry, and Therapeutic Applications of Nitric Oxide
Place of Presentation
仙台
Year and Date
2016-05-20
Related Report
Int'l Joint Research
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