| Project/Area Number |
16K15791
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Tokyo Dental College |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
小野寺 晶子 東京歯科大学, 歯学部, 講師 (90637662)
齋藤 暁子 東京歯科大学, 歯学部, 助教 (90722835)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | McCune-Albright 症候群 / iPS細胞 / GNAS / McCune-Albright症候群 / 線維性骨異形成症 / GNAS1 / 歯学 / 病理学 |
|---|
| Outline of Final Research Achievements |
Since distribution of the gene mutated cells in McCune-Albright syndrome (MAS) occurs depending somatic mosaicism, we applied gene editing technique using CRISPR/Cas9 system to generate human iPS cells exhibiting GNAS1 mutation observed in MAS. We obtained 2 clones exhibiting GAS1 mutation among 24 clones. These iPS cells showed undifferentiated markers and those identifying three germ layers. Thus we successfully established human iPS cells retaining GNAS1 mutaion observed in MAS.
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