Control of pulpal inflammation and hard tissue regeneration by miR-21, a modulator of cross-talk between inflammation and tissue regeneration

• Project/Area Number: 16K15795
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Conservative dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: KAWASHIMA Nobuyuki 東京医科歯科大学, 大学院医歯学総合研究科, 講師 (60272605)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: dental pulp cell / micro RNA / miR-21 / NFkB signaling / inflammation / LPS / signaling cascade / differentiation / マイクロRNA / miR21 / 歯髄炎 / 炎症性サイトカイン / 炎症制御 / 分化誘導 / NFkBシグナル / ERKシグナル / 歯髄幹細胞 / 炎症性メディエーター / マクロファージ / NFkB / 炎症 / 組織再生

Outline of Final Research Achievements

miR-21 was up-regulated in the inflamed rat dental pulp tissue, and lipopolysaccharides induced expression of miR-21 in the human dental pulp cells. miR-21 down-regulated NFkB signaling via degradation of TNF receptor-associated factor (TRAF) 6 and programmed cell death (PDCD) 4. miR-21 also promoted osteopontin expression. miR-21 may control the progress of pulpal inflammation and modulate the hard tissue formation in the dental pulp tissue.

Research Products

• [Journal Article] EDTA Treatment for Sodium Hypochlorite?treated Dentin Recovers Disturbed Attachment and Induces Differentiation of Mouse Dental Papilla Cells (2018)
  • Author(s): Hashimoto Kentaro、Kawashima Nobuyuki、Ichinose Shizuko、Nara Keisuke、Noda Sonoko、Okiji Takashi
  • Journal Title: Journal of Endodontics Volume: 44 Issue: 2 Pages: 256-262
  • DOI: 10.1016/j.joen.2017.11.003
  • Peer Reviewed
• [Journal Article] Transient receptor potential melastatin (TRPM) 8 is expressed in freshly isolated native human odontoblasts (2017)
  • Author(s): Tazawa Kento、Ikeda Hideharu、Kawashima Nobuyuki、Okiji Takashi
  • Journal Title: Archives of Oral Biology Volume: 75 Pages: 55-61
  • DOI: 10.1016/j.archoralbio.2016.12.007
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Properties of Dental Pulp?derived Mesenchymal Stem Cells and the Effects of Culture Conditions (2017)
  • Author(s): Kawashima Nobuyuki、Noda Sonoko、Yamamoto Mioko、Okiji Takashi
  • Journal Title: Journal of Endodontics Volume: 43 Issue: 9 Pages: S31-S34
  • DOI: 10.1016/j.joen.2017.06.004
  • Peer Reviewed
• [Journal Article] Odontoblasts: specialized hard-tissue-forming cells in the dentin-pulp complex (2016)
  • Author(s): Kawashima N and Okiji T
  • Journal Title: Congenit Anom (Kyoto) Volume: 56 Issue: 4 Pages: 144-153
  • DOI: 10.1111/cga.12169
  • NAID: 130008079156
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Meis2 Induces Odonto-/Osteoblastic Differentiation and Mineralization (2017)
  • Author(s): Kawashima N, Noda S, Hashimoto K, Saito M, Okiji T,
  • Organizer: IADR/AADR/CADR General Session & Exhibition
  • Int'l Joint Research
• [Presentation] Dense Culture Condition Promotes Human Dental Pulp Stem Cell (2017)
  • Author(s): Noda S, Kawashima N, Hashimoto K, Aramaki O, Okiji T
  • Organizer: IADR/AADR/CADR General Session & Exhibition
  • Int'l Joint Research
• [Presentation] ヒト歯髄細胞における炎症性メディエーター産生制御におけるHIF1a の役割 (2017)
  • Author(s): 藤井真由子，川島伸之，興地隆史
  • Organizer: 第59回歯科基礎医学会学術大会
• [Presentation] 培養密度が歯髄幹細胞の幹細胞特性に与える影響 (2017)
  • Author(s): 野田園子、川島伸之、橋本健太郎、山本弥生子、荒牧音、田上順次、興地隆史
  • Organizer: 第146回日本歯科保存学会2017年春季学術大会
• [Presentation] Mineral trioxide aggregate はLPS刺激マクロファージの機能をcalcium-sensing receptor を介して調節する (2017)
  • Author(s): 倉本将司、川島伸之、野崎浩佑、Bakhit AY、奈良圭介、藤井真由子、橋本健太郎、野田園子、興地隆史
  • Organizer: 第146回日本歯科保存学会2017年春季学術大会
• [Presentation] microRNA21 down-regulates the expression of inflammatory mediators in LPS-stimulated human dental pulp cells via an NF-κB dependent mechanism (2016)
  • Author(s): Nara K, Kawashima N, Noda S, Hashimoto K, Okiji T
  • Organizer: utumn Scientific Meeting (the 146th) and Joint Scientific Meeting of KACD-JACD (the 18th)
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2016-10-21
  • Int'l Joint Research
• [Presentation] 歯髄幹細胞の三次元培養における幹細胞マーカーの発現動態 (2016)
  • Author(s): 川島伸之 山本弥生子 野田園子 橋本健太郎 興地隆史
  • Organizer: 第23回日本歯科医学会総会
  • Place of Presentation: 福岡国際会議場，福岡
  • Year and Date: 2016-10-21
• [Presentation] Properties of dental pulp derived-mesenchymal stem cells and effects of culture conditions (2016)
  • Author(s): Kawashima N, Noda S, Hashimoto K, Yamamoto M, Okiji T
  • Organizer: IADR Pulp Biology and Regeneration Group Satellite Symposium,
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2016-06-26
  • Int'l Joint Research / Invited