| Project/Area Number |
16K15812
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
SUMITA Yoshinori 長崎大学, 医歯薬学総合研究科(歯学系), 准教授 (50456654)
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| Co-Investigator(Kenkyū-buntansha) |
朝比奈 泉 長崎大学, 医歯薬学総合研究科(歯学系), 教授 (30221039)
各務 秀明 松本歯科大学, 総合歯科医学研究所, 教授 (80242866)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 骨再生 / エクソソーム / 分化 / 間葉系幹細胞 / 再生医学 / 骨 |
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| Outline of Final Research Achievements |
This study aimed to analyze the function of MSC-derived exosomes (MSC-ex) that were harvested from the different stages of differentiation such as immature-, preosteoblastic differentiated-, osteoblastic differentiated-MSCs. In this study, we firstly analyzed the characters of these 3 different MSC-exes, and then evaluated the characteristic changes of MSCs after cultured in medium with these MSC-exes. As results, we found the osteoblastic differentiated-MSC-ex showed the increased expression of osteoblastic genes such as runx2 or alp. Furthermore, we confirmed these MSC-exes are taken into MSCs during 24 hours in culture, and the osteogenic mRNAs were upregulated in MSCs cultured with osteoblastic-differentiated-MSC-ex. In contrast, immature-MSC-ex showed the higher expression levels of mRNAs related to the vasculogenesis such as vegf. We are currently carrying out in vivo analyses for clarifying the actual usefulness of MSC-exes for bone regeneration.
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