Approach of production of dental epithelial cells from periodontal ligament mesenchymal cells for tooth regeneration
| Project/Area Number |
16K15813
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Iwate Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
西谷 直之 岩手医科大学, 薬学部, 教授 (10286867)
藤原 尚樹 岩手医科大学, 歯学部, 准教授 (20190100)
大津 圭史 岩手医科大学, 歯学部, 准教授 (60509066)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | ヘルトビッヒ上皮鞘 / 上皮間葉転換 / 歯の再生 / 間葉上皮転換 / セマフォリン / マラッセ上皮遺残 / 歯根膜 / エナメル上皮幹細胞 / エナメル上皮細胞 / マラッセ残存上皮 |
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| Outline of Final Research Achievements |
The aim of this project is to produce dental epithelial stem cells from mesenchymal cells in periodontal ligament by mesenchymal-epithelial transition (MET) and to utilize the epithelial cells for tooth regeneration. In order to obtain their cells, we tried to inhibit the epithelial-mesenchymal transition (EMT) and induce epithelial cells from EMT cells. As a result, Rho activator, Semaphorin4A and 4D, LPA (lysophospholipid) activate Rho signal and inhibited the EMT, and further induced MET and hyper-epithelium. But, it was difficult to obtain enough cell number of dental epithelial cells, it remains as further problem.
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Report
(3 results)
Research Products
(17 results)
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[Journal Article] Loss of Stemness, EMT, and Supernumerary Tooth Formation in Cebpb-/-Runx2+/- Murine Incisors.2018
Author(s)
Saito K, Takahashi K, Huang B, Asahara M, Kiso H, Togo Y, Tsukamoto H, Mishima S, Nagata M, Iida M, Tokita Y, Asai M, Shimizu A, Komori T, Harada H, MacDougall M, Sugai M, Bessho K.
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Journal Title
Scientific Reports
Volume: 8
Issue: 1
Pages: 5169-5169
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] <b>Hertwig’s epithelial root sheath cells contribute to formation of periodontal ligament through epithelial-mesenchymal transition by </b><b>TGF-β </b>2017
Author(s)
Itaya, S., Oka, K., Ogata, K., Tamura, S., Kira-Tatsuoka, M., Fujiwara, N., Otsu, K., Tsuruga, E., Ozaki, M., and Harada, H.
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Journal Title
Biomedical Research
Volume: 38
Issue: 1
Pages: 61-69
DOI
NAID
ISSN
0388-6107, 1880-313X
Related Report
Peer Reviewed / Open Access
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