• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of a new drug delivery system targeting the protein which cancer-specifically expresses

Research Project

Project/Area Number 16K15818
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Surgical dentistry
Research InstitutionChiba University

Principal Investigator

Tanzawa Hideki  千葉大学, 大学院医学研究院, 教授 (50236775)

Co-Investigator(Kenkyū-buntansha) 小河原 克訓  千葉大学, 大学院医学研究院, 特任研究員 (20372360)
坂本 洋右  千葉大学, 医学部附属病院, 講師 (50451745)
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsdrug delivery system / エキソソーム / 超遠心法 / 特異的吸着能 / 扁平上皮癌 / ファージディスプレイ法 / siRNA
Outline of Final Research Achievements

Two microarray analyses found candidate genes, OLR1 and EBI3, which expressed commonly among 5 squamous cell carcinoma cells (HSC2, HSC3, HSC4, Sa3, and H1), showing high expression on the cell membrane. There are those gene clones in the cDNA library, which established by our previous another study, and the clones were able to be available in the present experiment. The high expression of these genes in oral cancer cells were confirmed by qRT-PCR. The efficiency to induct the expression vector to fibroblast and/or dendritic cells was evaluated and the efficiency to produce the exosomes was also evaluated. Exosomes were successfully extracted from human fetal fibroblast cells and the specific adsorption ability to cancer cells of exosomes was confirmed.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2022-03-04  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi