Genomic surgery by genome editing of pathogenic mutant genes in oral maxillofacial disease-specific iPS cells
| Project/Area Number |
16K15823
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
Okamoto Tetsuji 広島大学, 医歯薬保健学研究科(歯), 教授 (00169153)
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| Co-Investigator(Kenkyū-buntansha) |
濱田 充子 広島大学, 病院(歯), 歯科診療医 (30760318)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 遺伝性顎顔面口腔疾患 / 疾患特異的iPS細胞 / 病原変異遺伝子 / 人工ヌクレアーゼ / ゲノム手術 / 無血清培養 / オーガノイド / von Recallinghausen病 / センダイウイルスベクター / フォンレッツクリングハウゼン病 / テラトーマ / 神経分化 / 軟骨・骨分化 / NF1遺伝子 / iPS細胞 / フィーダー細胞フリー培養 / 口腔顎顔面遺伝性疾患 / ゲノム編集 / CRISPR/Cas9 / 顎顔面口腔遺伝性疾患 |
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| Outline of Final Research Achievements |
iPSCs were established from peripheral lymphocytes of healthy subjects and patients with various inherited oral-maxillofacial diseases using serum-free medium hESF 9 and SeVdp, and their cellular characteristics and ability to differentiate into specific cell lineages were examined in serum-free organoid culture. In addition, we compare the differentiation potential to 3 germ layers and the structure of each induction tissue in iPSC- derived teratoma in the dorsal back of SCID mouse. We conducted a full genome analysis of DNA from patients with genetic diseases by the next generation sequencing (NGS), clarified pathogenic mutant genes and other mutations, and aimed to develop a new treatment method for the genetic disease.
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Report
(3 results)
Research Products
(23 results)
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[Journal Article] Eldecalcitol (ED-71), an analog of 1α,25(OH)2D3, inhibits the growth of squamous cell carcinoma (SCC) cells in vitro and in vivo by down-regulating expression of heparin-binding protein 17/fibroblast growth factor-binding protein-1 (HBp17/FGFBP-1) and FGF-22017
Author(s)
T. Shintani, F. Takatsu, S. N. Z. Rosli, E. Usui, Atsuko Hamada, K. Sumi, Y. Hayashido, S. Toratani, Tetsuji Okamoto
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Journal Title
In Vitro Cellular & Developmental Biology - Animal
Volume: 53
Issue: 9
Pages: 810-817
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Generation and maintenance of human induced pluripotent stem cells in serum-free and feeder-free culture condition-comparison of various target cells and virus vectors2016
Author(s)
E. Akagi, S. Yamasaki, A. Hamada, H. Nakatao, F. Obayashi, T. Yasui, Y. Taguchi, M. Ohtaka, K. Nishimura, M. Nakanishi, T. Okamoto
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Journal Title
Jpn. J. Tissue Cult. Soc. Dent. Res
Volume: 25(1),
Pages: 21-22
Related Report
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[Journal Article] Generation of Disease-specific human iPSCs in integration-, feeder-, and serum-free culture2016
Author(s)
A. Hamada, H. Nakatao, F. Obayashi, T. Yasui, E. Akagi, S. Yamasaki, S. Toratani, T. Okamoto
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Journal Title
Jpn. J. Tissue Cult. Soc. Dent. Res
Volume: 25(1),
Pages: 23-24
Related Report
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[Journal Article] Generation of cleidocranial dysplasia-specific human induced pluripotent stem cells in completely serum-, feeder-, and integration-free culture.2015
Author(s)
Yamasaki S, Hamada A, Akagi E, Nakatao H, Ohtaka M, Nishimura K, Nakanishi M, Toratani S, Okamoto T.
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Journal Title
In Vitro Cell Dev Biol Anim.
Volume: 52
Issue: 2
Pages: 252-264
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Generation of disease-specific human induced pluripotent stem (iPS) cells from dental pulp cells of a patient with Cleidocranial dysplasia in serum-, and feeder-free culture2016
Author(s)
A. Hamada, S. Yamasaki, E. Akagi, H. Nakatao, F. Obayashi, T. Fukutani, K. Koizumi, T. Hamana, T. Okamoto
Organizer
The 14th International Conference on cellular endocrinology
Place of Presentation
Hiroshima, Japan
Year and Date
2016-11-13
Related Report
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[Presentation] Establishment and characterization of normal and disease-specific human iPSCs in serum-, integration- and feeder-free culture, Plenary Symposium on Infinite Potential of Stem Cells,2016
Author(s)
S. Yamasaki, A. Hamada, H. Nakatao, E. Akagi, F. Ohbayashi, T. Fukutani, S. Toratani, T. Okamoto
Organizer
2016 World Congress on In Vitro Biology
Place of Presentation
San Diego, California, USA
Year and Date
2016-06-14
Related Report
Int'l Joint Research / Invited
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