Omic analysis from single cell circulating tumor cells of oral cancer

• Project/Area Number: 16K15833
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: National Cancer Center Japan
• Principal Investigator: Kazufumi Honda 国立研究開発法人国立がん研究センター, 研究所, ユニット長 (10260936)
• Co-Investigator(Kenkyū-buntansha): 森 泰昌 国立研究開発法人国立がん研究センター, 中央病院, 医員 (00296708) ; 吉本 世一 国立研究開発法人国立がん研究センター, 中央病院, 科長 (00462242)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 末梢循環腫瘍細胞 / 1細胞解析 / オミクス解析 / 末梢腫瘍循環細胞 / ゲノム解析 / 癌 / 外科 / 歯学

Outline of Final Research Achievements

Liquid biopsy has recently gained attention as a real-time monitoring method for patients with cancer. Circulating tumor cells (CTCs) and circulating tumor DNA (ctDNA) released into the bloodstream from both the primary tumor and metastases may reflect current tumor status.　Although it is considering that CTCs are consisting the heterogeneous of population, the biological behavior of single cell CTC has not identified yet. To identify behavior of single cell CTC, we developed the omics analysis of single cell CTC.

Research Products

• [Journal Article] Serum apolipoprotein A2 isoforms in autoimmune pancreatitis (2018)
  • Author(s): Kobayashi Takashi、Sato Yu、Nishiumi Shin、Yagi Yosuke、Sakai Arata、Shiomi Hideyuki、Masuda Atsuhiro、Okaya Shinobu、Kutsumi Hiromu、Yoshida Masaru、Honda Kazufumi
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 497 Issue: 3 Pages: 903-907
  • DOI: 10.1016/j.bbrc.2018.02.170
  • NAID: 120006604668
  • Peer Reviewed / Open Access
• [Journal Article] The peripheral immune status of granulocytic myeloid-derived suppressor cells correlates the survival in advanced gastric cancer patients receiving cisplatin-based chemotherapy (2017)
  • Author(s): Shoji Hirokazu、Tada Kohei、Kitano Shigehisa、Nishimura Takashi、Shimada Yasuhiro、Nagashima Kengo、Aoki Kazunori、Hiraoka Nobuyoshi、Honma Yoshitaka、Iwasa Satoru、Takashima Atsuo、Kato Ken、Boku Narikazu、Honda Kazufumi、Yamada Tesshi、Heike Yuji、Hamaguchi Tetsuya
  • Journal Title: Oncotarget Volume: 8 Issue: 56 Pages: 95083-95094
  • DOI: 10.18632/oncotarget.18297
  • Peer Reviewed / Open Access
• [Journal Article] Actinin-1 and actinin-4 play essential but distinct roles in invadopodia formation by carcinoma cells (2017)
  • Author(s): Yamaguchi Hideki、Ito Yuumi、Miura Nami、Nagamura Yuko、Nakabo Ayaka、Fukami Kiyoko、Honda Kazufumi、Sakai Ryuichi
  • Journal Title: European Journal of Cell Biology Volume: 96 Issue: 7 Pages: 685-694
  • DOI: 10.1016/j.ejcb.2017.07.005
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Actinin-4 protein overexpression as a predictive biomarker in adjuvant chemotherapy for resected lung adenocarcinoma. (2017)
  • Author(s): Shiraishi H, Fujiwara Y, Kakuya T, Tsuta K, Motoi N, Miura N, Watabe Y, Watanabe SI, Noro R, Nagashima K, Huang W, Yamada T, Asamura H, Ohe Y, Honda K.
  • Journal Title: Biomark Med. Volume: 11 Issue: 9 Pages: 721-731
  • DOI: 10.2217/bmm-2017-0150
  • Peer Reviewed / Open Access
• [Journal Article] Prognostic significance of gene amplification of ACTN4 in stage I and II oral tongue cancer. (2017)
  • Author(s): Kakuya T, Mori T, Yoshimoto S, Watabe Y, Miura N, Shoji H, Onidani K, Shibahara T, Honda K.
  • Journal Title: Int J Oral Maxillofac Surg. Volume: 46（8） Issue: 8 Pages: 968-976
  • DOI: 10.1016/j.ijom.2017.03.001
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Identification of highly sensitive biomarkers that can aid the early detection of pancreatic cancer using GC/MS/MS-based targeted metabolomics. (2016)
  • Author(s): Hirata Y., Ioka T., et al.
  • Journal Title: Clin. Chim. Acta. Volume: Feb. 16 Pages: 98-104
  • DOI: 10.1016/j.cca.2017.02.011
  • NAID: 120006375910
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Next-generation sequencing of circulating tumor cells isolated from peripheral blood of patients with head and neck or gastrointestinal cancer, (2017)
  • Author(s): Kaoru Onidani, Seiichi Yoshimoto, Nami Miura, Hirokazu Shoji, Ken Kato, Takahiko Shibahara, Kazufumi Honda
  • Organizer: ESMO Asia2017
  • Int'l Joint Research
• [Presentation] 次世代シーケンサーによる血中循環腫瘍細胞(CTCs)の遺伝子変異プロファイリング (2017)
  • Author(s): 鬼谷 薫、吉本世一、三浦奈美、庄司広和、加藤 健、柴原孝彦、本田一文
  • Organizer: 第37回日本分子腫瘍マーカー研究会
• [Presentation] Next-generation sequencing of circulating tumor cells isolated from peripheral blood of patients with head and neck or gastrointestinal cancer (2017)
  • Author(s): Kaoru Onidani, Seiichi Yoshimoto, Nami Miura, Hirokazu Shoji, Ken Kato, Takahiko Shibahara, Kazufumi Honda
  • Organizer: 2017年度生命科学系学会合同年次大会
• [Presentation] 次世代シーケンサーによる血中循環腫瘍細胞(CTCs)の遺伝子プロファイリング (2017)
  • Author(s): 鬼谷 薫、柴原孝彦、本田一文
  • Organizer: 第62回日本口腔外科学会総会・学術大会
• [Presentation] Prognostic significance of gene amplification of ACTN4 in stage I and II oral tongue cancer (2017)
  • Author(s): Kaoru Onidani, Takanori Kakuya, Nami Miura, Hirokazu Shoji, Takahiko Shibahara, Kazufumi Honda
  • Organizer: 第76回日本癌学会