Translational research to overcome chromosomal abnormality using deciduous teeth

• Project/Area Number: 16K15839
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Kyushu University
• Principal Investigator: Nonaka Kazuaki 九州大学, 歯学研究院, 教授 (90128067)
• Co-Investigator(Kenkyū-buntansha): 佐藤 浩 九州大学, 歯学研究院, 助教 (00421313) ; 加藤 大樹 九州大学, 医学(系)研究科(研究院), 研究員 (30452709) ; 廣藤 雄太 (広藤 雄太) 九州大学, 歯学研究院, 助教 (80759746) ; 山座 治義 九州大学, 歯学研究院, 准教授 (30336151) ; 増田 啓次 九州大学, 大学病院, 講師 (60392122)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: ダウン症 / ゲノム編集 / 乳歯由来幹細胞 / ヒト脱落乳歯由来幹細胞 / ダウン症候群 / 歯髄幹細胞 / トランスレーショナルリサーチ

Outline of Final Research Achievements

We examined the pathology of down syndrome using stem cells from human exfoliated deciduous teeth (SHED) derived from a patient with down syndrome. Furthermore, we attempted to develop a system to eliminate excess chromosome 21 by genome editing. We revealed that neurodevelopment ability of SHED derived from a patient was lower than SHED derived from a typically developing child. In addition, we established a system to eliminate a targeted chromosome.

Research Products

• [Journal Article] Mitochondria Regulate the Differentiation of Stem Cells from Human Exfoliated Deciduous Teeth (2017)
  • Author(s): Kato H, Pham TMT, Yamaza H, Masuda K, Hirofuji Y, Han X, Sato H, Taguchi T, Nonaka K.
  • Journal Title: Cell Structure and Function Volume: 42 Pages: 105-116
  • NAID: 130005982848
  • Peer Reviewed / Open Access
• [Journal Article] Direct effects of mitochondrial dysfunction on poor bone health in Leigh syndrome (2017)
  • Author(s): Kato Hiroki、Han Xu、Yamaza Haruyoshi、Masuda Keiji、Hirofuji Yuta、Sato Hiroshi、Pham Thanh Thi Mai、Taguchi Tomoaki、Nonaka Kazuaki
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 493 Issue: 1 Pages: 207-212
  • DOI: 10.1016/j.bbrc.2017.09.045
  • Peer Reviewed / Open Access
• [Journal Article] Engineering of Systematic Elimination of a Targeted Chromosome in Human Cells. (2017)
  • Author(s): Sato H, Kato H, Yamaza H, Masuda K, Nguyen HT, Pham TT, Han X, Hirofuji Y, Nonaka K
  • Journal Title: Biomed Res Int Volume: - Pages: 1-5
  • DOI: 10.1155/2017/6037159
  • Peer Reviewed / Open Access
• [Presentation] HeLa細胞における21番染色体トリソミーのダイゾミー化 (2017)
  • Author(s): 佐藤 浩、加藤 大樹、山座 治義、増田 啓次、Huong Nguyen、Thanh Pham、韓 旭、廣藤 雄太、野中 和明
  • Organizer: 2017年度生命科学系学会合同年次会
• [Presentation] Impairment of Dopaminergic Neuronal Development in Down Syndrome (2017)
  • Author(s): Thanh Thi Mai Pham, Haruyoshi Yamaza, Keiji Masuda, Yuta Hirofuji, Kazuaki Nonaka
  • Organizer: 第55回日本小児歯科学会大会
• [Presentation] Understanding of Neuropathology in Down Syndrome (2017)
  • Author(s): Thanh Thi Mai Pham, Hiroki Kato, Haruyoshi Yamaza, Keiji Masuda, Yuta Hirofuji, Hiroshi Sato, Huong Thi Nguyen Nguyen, Tomoaki Taguchi, Kazuaki Nonaka
  • Organizer: The 28th Fukuoka International Symposium on Pediatric/ Maternal-Child Health Research
  • Int'l Joint Research
• [Presentation] ミトコンドリアによる乳歯由来歯髄幹細胞の神経分化制御 (2017)
  • Author(s): Hiroki Kato, Thanh Thi Mai Pham, Xu Han, Yuta Hirofuji, Haruyoshi Yamaza, Keiji Masuda, Hiroshi Sato, Tomoaki Taguchi, Kazuaki Nonaka
  • Organizer: 第69回細胞生物学会大会