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Comprehensive analysis of protein in gingival crevicular fluids and its association with neutrophil extracellular trap

Research Project

Project/Area Number 16K15847
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Periodontology
Research InstitutionShowa University

Principal Investigator

YAMAMOTO MATSUO  昭和大学, 歯学部, 教授 (50332896)

Co-Investigator(Kenkyū-buntansha) 板部 洋之  昭和大学, 薬学部, 教授 (30203079)
小出 容子  昭和大学, 歯学部, 助教 (40407466)
美島 健二  昭和大学, 歯学部, 教授 (50275343)
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords歯肉溝滲出液 / 細胞外トラップ / 質量分析 / 好中球 / LPS / NETs / NETosis / シトルリン化 / 歯周病 / 歯肉溝浸出液 / 好中球細胞外トラップ / サイトカイン / 炎症
Outline of Final Research Achievements

It is thought that neutrophils inhibit not only phagocytosis but also invasion of periodontal pathogenic bacteria by extracellular traps in order to prevent invasion of oral bacteria into the soft tissue in the gingival sulcus. However, there remains unclear about the control mechanism of NETosis in the gingival sulcus. The protein components contained in the gingival crevicular fluid were analyzed by SDS-PAGE method and mass spectrometry. They showd that GCF includes lactoferrin, serotransferrin, S100A8, 9, IL-1beta and complement which are known as a trigger molecule induced by neutrophil NETosis. On the other hand, NETosis was induced in neutrophils and neutrophil-like cell HL-60 when they were stimulated by Porphyromonas gingivalis LPS. Taken together, it is suggested that NETosis may occur in neutrophils by stimulation of bacterial factors and GCF components in the gingival sulcus.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (2 results)

All 2016

All Presentation (2 results)

  • [Presentation] 乳歯と永久歯の歯肉溝滲出液中に含まれるタンパク質の網羅的解析(第2報)2016

    • Author(s)
      守屋佑美、小浜孝士、小出容子、野口江美子、石塚元規、竹丸真以、板部洋之、山本松男
    • Organizer
      第59回春季日本歯周病学会学術大会(鹿児島)
    • Place of Presentation
      鹿児島
    • Year and Date
      2016-05-20
    • Related Report
      2016 Research-status Report
  • [Presentation] 乳歯と永久歯の歯肉溝滲出液における好中球機能の検討2016

    • Author(s)
      守屋佑美、小浜孝士、杉山智美、遠藤由美子、小出容子、竹丸真以、井上美津子、板部洋之、山本松男
    • Organizer
      第330回昭和大学学士会例会
    • Place of Presentation
      東京
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2019-03-29  

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