The model of gingival sulcus made from Revival gums: the pre-clinical study for an animal experiment

• Project/Area Number: 16K15858
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Social dentistry
• Research Institution: The Nippon Dental University
• Principal Investigator: Yaegaki Ken 日本歯科大学, 生命歯学部, 教授 (40166468)
• Co-Investigator(Kenkyū-buntansha): 田中 とも子 日本歯科大学, 生命歯学部, 准教授 (70307958)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: バイオフィルム / 歯肉溝 / 歯周病 / 歯周疾患 / Red complex / 前臨床研究 / Red Complex / 歯肉上皮 / in vitro

Outline of Final Research Achievements

The purpose of this study is how to establish a gingival sulcus model by reclaimed gums and to drive the pre-clinical study.
The microorganisms were Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Streptococcus gordonii. We tried biofilm formation on the section of a human tooth with the TYGVS culture medium added N-acetylmuramic acid. The biofilm formation was confirmed by the SEM observation and the bacterial flora analyses.
And the stem cells segregation became a simple method by the magnetic separation. But reproduction of an artificial gingival sulcus didn't come to realization.

Research Products

• [Presentation] 歯周病原細菌による人工バイオフィルム形成に関する基礎的研究２ (2017)
  • Author(s): 田中とも子
  • Organizer: 日本口腔衛生学会