| Project/Area Number |
16K16191
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Risk sciences of radiation and chemicals
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 放射線 / 精子形成 / 精巣 / 精巣器官培養 / 炎症 / 細胞死 / 生殖細胞 / インフラマソーム |
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| Outline of Final Research Achievements |
The aim of this study was to investigate the effect of inflammation after γ-ray irradiation on the viability of gonocytes in prenatal mice. IL-1β and IL-6 mRNA expression increased in testes exposed to γ-rays, suggesting that inflammation occurred in testes after γ-ray irradiation. When ASC-deficient mice were exposed to γ-ray on Day 16.5 post-coitus, the male germ cells had already disappeared on Day 60 post-partum. In the organ culture of testis, meanwhile, addition of lipopolysaccharide into the culture medium disturbed the development of testis in vitro. These results demonstrated that inflammation induced by γ-ray irradiation was not the cause of cell death in gonocytes. However, it seemed that development of juvenile mouse testis affected by inflammatory responses.
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