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Detection of retinoic acid recetptor agonists in river water in Japan

Research Project

Project/Area Number 16K16217
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Environmental risk control and evaluation
Research InstitutionKitasato University

Principal Investigator

sawada kazuko  北里大学, 医療衛生学部, 特別研究員 (00772485)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsレチノイン酸 / レチノイン酸受容体 / アゴニスト / 河川水 / 酵母two-hybrid法 / レチノイン酸受容体(RAR) / ヒトRAR / メダカRAR / RARアゴニスト / 河川
Outline of Final Research Achievements

This study aimed to elucidate the occurrence of the agonists on human and medaka RAR in river water environments in Japan. To clarify the temporal variation of RAR agonist contamination in Nakatsu River, investigations of the RAR agonistic activity were carried out on 4 distinct date from May 2016 to February 2017 by the yeast two-hybrid assay targeting human and medaka RAR. To characterize the RAR agonists, samples collected from several sampling stations during different periods were fractionated by reverse-phase HPLC. To identify the causative compounds, the concentrations of RAs and 4-oxo-RAs in the samples were analyzed by LC/MS. To evaluate affinities for human and medaka RAR, RAR agonistic activities of several retinoids were measured by the yeast two-hybrid assay. The atRA equivalency factors of several retinoids were estimated by comparing their EC50 values with that of atRA.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (3 results)

All 2017

All Presentation (3 results) (of which Int'l Joint Research: 1 results)

  • [Presentation] ヒトおよびメダカの受容体を用いた河川水中のレチノイン酸受容体アゴニスト汚染の実態調査2017

    • Author(s)
      澤田和子
    • Organizer
      第51回日本水環境学会年会
    • Place of Presentation
      熊本大学
    • Year and Date
      2017-03-15
    • Related Report
      2016 Research-status Report
  • [Presentation] 河川水中におけるレチノイン酸受容体アゴニスト汚染の実態解明2017

    • Author(s)
      澤田和子
    • Organizer
      日本水処理生物学会第54回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Detection of Human and Medaka Retinoic Acid Receptor Agonistic Activities in River Water in Japan2017

    • Author(s)
      Kazuko Sawada
    • Organizer
      SETAC North America
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research

URL: 

Published: 2016-04-21   Modified: 2019-03-29  

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