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Real-time imaging of fouling-related biofilm in wastewater treatment

Research Project

Project/Area Number 16K16233
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Design and evaluation of sustainable and environmental conscious system
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

Inaba Tomohiro  国立研究開発法人産業技術総合研究所, 環境管理研究部門, 研究員 (90760439)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywordsバイオフィルム / バイオファウリング / 水処理再生 / 水処理 / 膜分離活性汚泥法 / MBR / 水資源
Outline of Final Research Achievements

In recent years, global water shortages have become more serious and the need for reclaimed water is rapidly rising. By the membrane bioreactor (MBR)- reverse osmosis (RO) membrane method, high-quality reclaimed water can be obtained, but the clogging of water treatment membrane, called membrane fouling, is the most serious problem in MBR-RO system. Although the details of the mechanism of the membrane fouling are still unknown, microbial aggregates, i.e. biofilms, are considered to be one major cause of membrane fouling. In this study, the structure of fouling related biofilm was investigated using non-destructive confocal reflection microscopy and high-throughput sequencing of 16S rRNA genes. The results of combined analysis demonstrated that the architectures, chemical components and microbiomes of the biofilms on fouled membranes were tightly associated with one another and differed considerably depending on the organic loading conditions.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (11 results)

All 2018 2017 2016 Other

All Journal Article (5 results) (of which Peer Reviewed: 3 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (5 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results) Remarks (1 results)

  • [Journal Article] Revealing sludge and biofilm microbiomes in membrane bioreactor treating piggery wastewater by non-destructive microscopy and 16S rRNA gene sequencing2018

    • Author(s)
      Inaba Tomohiro、Hori Tomoyuki、Navarro Ronald R.、Ogata Atsushi、Hanajima Dai、Habe Hiroshi
    • Journal Title

      Chemical Engineering Journal

      Volume: 331 Pages: 75-83

    • DOI

      10.1016/j.cej.2017.08.095

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Eukaryotic Microbiomes of Membrane-Attached Biofilms in Membrane Bioreactors Analyzed by High-Throughput Sequencing and Microscopic Observations2018

    • Author(s)
      Tomohiro Inaba, Tomoyuki Hori, Yuya Sato, Tomo Aoyagi, Dai Hanajima, Atsushi Ogata, Hiroshi Habe
    • Journal Title

      Microbes and Environments

      Volume: 33 Issue: 1 Pages: 98-101

    • DOI

      10.1264/jsme2.ME17112

    • NAID

      130006581804

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 水処理プロセスにおけるバイオフィルム2018

    • Author(s)
      稲葉知大
    • Journal Title

      臨床と微生物

      Volume: 45 Pages: 1-6

    • Related Report
      2017 Annual Research Report
  • [Journal Article] 誰が膜を詰まらせるのか?2017

    • Author(s)
      稲葉知大
    • Journal Title

      生物工学会誌

      Volume: 45 Pages: 204-204

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Architecture, component, and microbiome of biofilm involved in the fouling of membrane bioreactors2017

    • Author(s)
      Tomohiro Inaba, Tomoyuki Hori, Hidenobu Aizawa, Atsushi Ogata and Hiroshi Habe
    • Journal Title

      npj Biofilms and Microbiomes

      Volume: 3 Issue: 1 Pages: 1-8

    • DOI

      10.1038/s41522-016-0010-1

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 次世代シークエンスと反射顕微鏡法との融合による水処理膜ファウリング機構の解明2017

    • Author(s)
      稲葉知大,堀知行,愛澤秀信,尾形敦,羽部浩
    • Organizer
      第20回日本水環境学会シンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] 畜産廃水処理MBRの安定化に関与する微生物群集の網羅的解析2017

    • Author(s)
      稲葉知大,堀知行,佐藤由也,青柳智,尾形敦,羽部浩
    • Organizer
      第52回日本水環境学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] The Membrane Fouling Biofilm: An Extreme Environment in Wastewater Treatment and Reclamation2016

    • Author(s)
      稲葉 知大、堀 知行、Navarro R Ronald、愛澤 秀信、尾形 敦、羽部 浩
    • Organizer
      Extremophiles 2016
    • Place of Presentation
      京都大学(京都府)
    • Year and Date
      2016-09-13
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 水処理再生と微生物の凝集2016

    • Author(s)
      稲葉 知大
    • Organizer
      界面動電現象研究会2016年サマースクール
    • Place of Presentation
      筑波大学(茨城県)
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 水処理再生技術とバイオフィルム2016

    • Author(s)
      稲葉 知大
    • Organizer
      ImPACTワークショップ「産業化に向けたバイオフィルム研究とクオラムクエンチング酵素工学」
    • Place of Presentation
      TKP市ヶ谷カンファレンスセンター(東京都)
    • Related Report
      2016 Research-status Report
    • Invited
  • [Remarks] 活性汚泥による水処理膜の閉塞を新たな手法で解析

    • URL

      http://www.aist.go.jp/aist_j/press_release/pr2017/pr20170223/pr20170223.html

    • Related Report
      2017 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2019-03-29  

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