Analysis of the mechanism of anti-diabetic effect by polyacetylenes from the Apiaceae vegetables.

• Project/Area Number: 16K16284
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Eating habits
• Research Institution: Iwate Medical University
• Principal Investigator: YOSHIDA Jun 岩手医科大学, 教養教育センター, 助教 (20611007)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: ポリアセチレン化合物 / falcarindiol / GSK-3β / セリ科植物 / 糖新生 / 機能性物質 / インスリン / 糖尿病

Outline of Final Research Achievements

In this study, we investigated the mechanism of the anti-diabetic effect of falcarindiol isolated from Apiaceae family vegetables. Falcarindiol suppressed glucose production in rat hepatoma H4ⅡE cells. Falcarindiol also increased the level of inhibitory phosphorylation of GSK-3β in H4IIE cells. These results indicate that several Apiaceae family vegetables containing falcarindiol could be a useful food ingredient against type-2 diabetes.

Research Products

• [Journal Article] Ca2+-Signal Transduction Inhibitors, Kujiol A and Kujigamberol B, Isolated from Kuji Amber Using a Mutant Yeast (2018)
  • Author(s): Uchida Takeshi、Koshino Hiroyuki、Takahashi Shunya、Shimizu Eisaku、Takahashi Honoka、Yoshida Jun、Shinden Hisao、Tsujimura Maiko、Kofujita Hisayoshi、Uesugi Shota、Kimura Ken-ichi
  • Journal Title: Journal of Natural Products Volume: 81 Issue: 4 Pages: 1070-1074
  • DOI: 10.1021/acs.jnatprod.7b00922
  • Peer Reviewed
• [Journal Article] New eremophilane and dichlororesorcinol derivatives produced by endophytes isolated from Ficus ampelas. (2017)
  • Author(s): Shiono Y., Muslihah I. N., Suzuki T., Ariefta R. N., Anwar C., Handojo Hadi Nurjanto H. H., Aboshi T., Murayama T., Tawaraya K., Koseki T., Yoshida J., Usukhbayar N., Uesugi S., Kimura K.
  • Journal Title: The Journal of Antibiotics Volume: 70 Issue: 12 Pages: 1133-1137
  • DOI: 10.1038/ja.2017.125
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] (4Z,15Z)-Octadecadienoic acid inhibits glycogen synthase kinase-3β and glucose production in H4IIE cells. (2017)
  • Author(s): Yoshida J., Uesugi S., Kawamura T., Kimura K., Hu D., Xia S., Toyooka N., Ohnishi M., Kawashima H.
  • Journal Title: Lipids Volume: 53(3) Issue: 3 Pages: 295-301
  • DOI: 10.1007/s11745-017-4236-3
  • Peer Reviewed
• [Journal Article] Yeast Ca2+-signal transduction inhibitors isolated from Dominican amber prevent the degranulation of RBL-2H3 cells through the inhibition of Ca2+-influx (2016)
  • Author(s): Tomomi Abe, Miki Kobayashi, Yusuke Okawa, Tomoki Inui, Jun Yoshida, Hironori Higashio, Hisao Shinden, Shota Uesugi, Hiroyuki Koshino, Ken-ichi Kimura
  • Journal Title: Fitoterapia Volume: 113 Pages: 188-194
  • DOI: 10.1016/j.fitote.2016.07.018
  • Peer Reviewed
• [Journal Article] GSK-3β inhibitory activities of novel dichroloresorcinol derivatives from Cosmospora vilior isolated from a mangrove plant. (2016)
  • Author(s): Shiono Y., Miyazaki N., Murayama T., Koseki T., Harizon, Katja D. G., Supratman U., Nakata J., Kakihara Y., Saeki M., Yoshida J., Uesugi S., Kimura K.
  • Journal Title: Phytochemistry Letters Volume: 18 Pages: 122-127
  • Peer Reviewed
• [Presentation] セリ科物植由来GSK-3β阻害物質falcarindiolの肝がん由来細胞における作用特性の解析 (2017)
  • Author(s): 吉田 潤、木村 賢一
  • Organizer: 日本農芸化学会2017年度大会
  • Place of Presentation: 京都女子大学(京都府京都市)
  • Year and Date: 2017-03-17
• [Presentation] ヒドロキシ脂肪酸ricinoleic acidのグルコース産生抑制作用の解析 (2017)
  • Author(s): 吉田 潤、伊藤 芳明、木村 賢一
  • Organizer: 日本農芸化学会東北支部第152回大会
• [Presentation] 神経突起伸長作用を有する非メチレン中断型ジエン酸の新たな生物活性探索 (2016)
  • Author(s): 吉田 潤、上杉 祥太、川村 哲晃、木村 賢一、Dawei Hu、Shuang Xia、豊岡 尚樹、大西 正男、川島 英城
  • Organizer: 日本油化学会第55回年会
  • Place of Presentation: 奈良女子大学(奈良県奈良市)
  • Year and Date: 2016-09-07
• [Presentation] ヒマシ油由来ヒドロキシ脂肪酸ricinoleic acidのカルシニューリン阻害活性 (2016)
  • Author(s): 吉田 潤、大川 佑介、小山 卓矢、上杉 祥太、木村 賢一
  • Organizer: 日本農芸化学会東北支部第151回大会
  • Place of Presentation: 山形大学農学部(山形県鶴岡市)