Molecular tension sensors visualize cell mechanical signal under mechanical stress

• Project/Area Number: 16K16387
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Okayama University
• Principal Investigator: Morimatsu Masatoshi 岡山大学, 医歯薬学総合研究科, 助教 (70580934)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: メカノシグナル / 分子張力センサー / 機械刺激 / ずり応力 / 細胞接着斑 / メカノバイオロジー / シグナル伝達 / shear stress / 生物・生体工学 / 生物物理

Outline of Final Research Achievements

We developed the shear stress applying system on the microscope and visualized focal adhesions motion and mechanical signal on real time. Our system revealed that focal adhesions and high mechanical signal are aligned to the parallel direction of flow direction. These results suggest integrin molecules use catch bond system.

Research Products

• [Int'l Joint Research] Stanford University(米国)
• [Journal Article] Activity-Dependent Dynamics of the Transcription Factor of cAMP-Response Element Binding Protein in Cortical Neurons Revealed by Single-Molecule Imaging (2017)
  • Author(s): Hironobu Kitagawa, Noriyuki Sugo, Masatoshi Morimatsu, Yoshiyuki Arai, Toshio Yanagida and Nobuhiko Yamamoto
  • Journal Title: Journal of Neuroscience Volume: 37 (1) Pages: 110-110
  • Peer Reviewed
• [Journal Article] Single Molecule Force Measurements in Living Cells Reveal a Minimally Tensioned Integrin State (2016)
  • Author(s): Alice C. Chang, Armen H. Mekhdjian, Masatoshi Morimatsu, Aleksandra Kirillovna Denisin, Beth L. Pruitt, and Alexander R. Dunn
  • Journal Title: ACS Nano Volume: 10(12) Issue: 12 Pages: 1074510752-1074510752
  • DOI: 10.1021/acsnano.6b03314
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Imaging of cell mechanics under high gravity by rotational microscope (2017)
  • Author(s): Masatoshi Morimatsu, Ayano Fujita, Ken Takahashi, Keiji Naruse
  • Organizer: Biophysical Society 61th Annual Meeting
  • Place of Presentation: New Orleans, LA, USA
  • Year and Date: 2017-02-11
  • Int'l Joint Research
• [Presentation] Controlling and visualizing the force clarify the cellular mechanosensing mechanism (2017)
  • Author(s): 森松賢順
  • Organizer: International Meeting on Biomolecules under Pressure (IMBP)
  • Int'l Joint Research / Invited
• [Presentation] 細胞の機械刺激応答の解明に向けた定量技術の開発 (2017)
  • Author(s): 森松賢順
  • Organizer: SaMPLセミナー
  • Invited
• [Presentation] メカノメディスンへの新展開 (2017)
  • Author(s): 森松賢順 成瀬恵治
  • Organizer: メカノバイオロジー学会
• [Presentation] High hydrostatic pressure induces cytoskeletal organization and signal transduction (2016)
  • Author(s): Masatoshi Morimatsu, Ayano Fujita, Ken Takahashi, Keiji Naruse
  • Organizer: 2016 American Society for Cell Biology Annual Meeting
  • Place of Presentation: San Francisco, CA, USA
  • Year and Date: 2016-12-03
  • Int'l Joint Research
• [Presentation] Hydrostatic pressure induces the signal transduction of MAPK pathway (2016)
  • Author(s): Masatoshi Morimatsu, Ayano Fujita, Ken Takahashi, Keiji Naruse
  • Organizer: Mechanobiology of Diseas
  • Place of Presentation: Singapore
  • Year and Date: 2016-09-27
  • Int'l Joint Research