| Project/Area Number |
16K16399
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
ARISAKA Yoshinori 東京医科歯科大学, 生体材料工学研究所, 助教 (70590115)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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| Keywords | ポリロタキサン / 成長因子 / 超分子 |
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| Outline of Final Research Achievements |
We prepared growth factor-immobilized polyrotaxane (PRX) surfaces and tried to regulate cellular functions by surface-immobilizing growth factor and modulating surface molecular mobility. Previously, it has been reported that sulfated PRXs form complexes with bone morphogenetic proteins (BMP-2). In the present study, sulfated PRX surfaces with immobilized BMP-2 were designed. It was revealed that the combination of surface-immobilization of BMP-2 and low molecular mobility of PRXs significantly promotes osteoblast differentiation of preosteoblasts. This suggests that regulation of molecular mobility and surface-immobilization of BMP-2 synergistically enhanced osteoblast differentiation. Similarly, it was revealed that the PRX surface is also useful in controlling the cellular functions of mesenchymal stem cells, liver-derived cells, vascular endothelial cells.
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| Academic Significance and Societal Importance of the Research Achievements |
環状分子と線状高分子とからなるポリロタキサンを被膜した表面(ポリロタキサン表面)において、ポリロタキサン骨格に由来する表面分子可動性によって接着細胞の機能が調節できることを報告している。本研究課題では、このようなポリロタキサン表面と成長因子をインテグレーションすることによって、さらに高度な細胞機能操作が可能となることを明らかにした。
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