Identification of electrostimulation and blood flow restriction protocols for skeletal muscle hypertrophy

• Project/Area Number: 16K16564
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Sports science
• Research Institution: Juntendo University
• Principal Investigator: NATSUME TOSHIHARU 順天堂大学, スポーツ健康科学研究科, 特任助教 (90761841)
• Research Collaborator: NAITO Hisashi
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: 筋疲労 / パンプアップ / 筋厚 / 筋肥大 / 筋力 / 血流制限 / 電気刺激 / 筋横断面積 / 筋電気刺激

Outline of Final Research Achievements

The purpose of present study was to reveal appropriate neuromuscular electrical stimulation (NMES) and blood flow restriction (BFR) protocols for muscle hypertrophy. We considered these protocols from the point of view of muscle swelling and muscle fatigue immediately after these treatments. According to the results, NMES (frequency, 20Hz; Pulse duration, 500μs; duty cycle, 4 s on: 8 s off) effectively caused muscle swelling and muscle fatigue when combined with 50% of resting arterial occlusion pressure, compared with other conditions. Moreover, the protocol induced muscle hypertrophy after 2 weeks of training than previous NMES with BFR training protocol.

Academic Significance and Societal Importance of the Research Achievements

筋電気刺激と血流制限を組み合わせたトレーニング方法は低体力者でも実施することが可能である。しかしながら効果的に筋肥大や筋力増加を導く至適条件については明らかでない。本研究によって筋力や筋量を増加する至適な実施条件が明らかとなれば疾患や傷害を有する人を対象としたトレーニングの実用化に大きく貢献することができる。

Research Products

• [Journal Article] Electromyostimulation with blood flow restriction enhances activation of mTOR and MAPK signaling pathways in rat gastrocnemius muscles (2018)
  • Author(s): Natsume Toshiharu、Yoshihara Toshinori、Naito Hisashi
  • Journal Title: Applied Physiology, Nutrition, and Metabolism Volume: 6 Issue: 6 Pages: 1-8
  • DOI: 10.1139/apnm-2018-0384
  • Peer Reviewed
• [Journal Article] Effects of training intensity in electromyostimulation on human skeletal muscle (2018)
  • Author(s): Natsume Toshiharu、Ozaki Hayao、Kakigi Ryo、Kobayashi Hiroyuki、Naito Hisashi
  • Journal Title: European Journal of Applied Physiology Volume: 118 Issue: 7 Pages: 1339-1347
  • DOI: 10.1007/s00421-018-3866-3
  • Peer Reviewed
• [Journal Article] Neuromuscular electrical stimulation with blood flow restriction increases serum growth hormone concentration (2018)
  • Author(s): Natsume T, Ozaki H, Saito AI, Naito H
  • Journal Title: Gazzetta medica Italiana Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Effects of single drop sets resistance training on increase muscle CSA, strength, and endurance: a pilot study (2018)
  • Author(s): Ozaki H, Kubota A, Natsume T, Loenneke JP, Abe T, Machida S, Naito H
  • Journal Title: Journal of Sports Sciences Volume: 36 Issue: 6 Pages: 691-696
  • DOI: 10.1080/02640414.2017.1331042
  • Peer Reviewed
• [Journal Article] Acute changes in blood lactate concentration, muscle thickness and strength after walking with blood flow restriction in older adults (2016)
  • Author(s): Natsume T, Ozaki H, Nakagata T, Machida S, Naito H
  • Journal Title: Juntendo Medical Journal Volume: 62 Pages: 237-242
  • NAID: 130005865669
• [Journal Article] The effects of the combination of increased physical acyivity with walking with blood flow restriction on leg muscle thickness and walking performance in older adults (2016)
  • Author(s): Ozaki H, Nakagata T, Natsume T, Machida S, Naito H
  • Journal Title: Juntendo Medical Journal Volume: 62 Pages: 206-210
• [Journal Article] Effect of 6-month walking and stair climbing exercise program and walking with blood flow restriction on body composition and hemoglobin A1c levels in elderly people (2016)
  • Author(s): Yoshihara T, Ozaki H, Nakagata T, Natsume T, Machida S, Naito H
  • Journal Title: Juntendo Medical Journal Volume: 62 Pages: 231-235
  • NAID: 130005865672
• [Presentation] Electromyostimulation with blood flow restriction enhances activation of mTOR and MAPK signalling pathways in rat gastrocnemius muscles (2018)
  • Author(s): Natsume T, Yoshihara T, Naito H
  • Organizer: Experimental Biology 2018
  • Int'l Joint Research
• [Presentation] Long-term Physical Inactivity Exacerbates Hindlimb Unloading-induced Soleus Muscle Atrophy In Young Rats (2018)
  • Author(s): Yoshihara T, Tsuzuki T, Chang SW, Natsume T, Kakigi R, Ichinoseki-Sekine N, Machida S, Sugiura T, Naito H
  • Organizer: ACSM's 65th Annual Meeting
  • Int'l Joint Research
• [Presentation] 筋電気刺激の刺激強度の違いが筋肥大に及ぼす影響 (2017)
  • Author(s): 棗寿喜, 尾崎隼朗, 柿木亮, 小林裕幸, 内藤久士
  • Organizer: 第７２回日本体力医学会大会
• [Presentation] 日本と中国における４歳幼児の全身各部位の筋厚と身体活動量に関する研究 (2017)
  • Author(s): トウホウウ, 尾崎隼朗, 棗寿喜, 陸大江, 近藤浩晃, 町田修一, 内藤久士
  • Organizer: 第７２回日本体力医学会大会
• [Presentation] 幼児における全身各部位の筋厚および皮脂厚の性差と年齢差 (2017)
  • Author(s): 尾崎隼朗, トウホウウ, 棗寿喜, 尾崎美那貴, 近藤浩晃, 町田修一, 内藤久士
  • Organizer: 第７２回日本体力医学会大会
• [Presentation] サッカーが高齢者男性における可視骨格筋の形態及び機能に及ぼす影響 (2017)
  • Author(s): 石原美彦, 尾崎隼朗, 棗寿喜, 石田真大, 井口祐貴, 吉村雅文, 町田修一, 内藤久士
  • Organizer: 第７２回日本体力医学会大会
• [Presentation] Resistance exercise attenuates locomotive syndrome risk in older adults. (2016)
  • Author(s): Natsume T, Ozaki H, Nakagata H, Yoshihara T, Kitada T, Ishihara Y, Ishibashi M, Machida S, Naito H
  • Organizer: 10th International Conference on Strength Training
  • Place of Presentation: 京都アバンティ, 京都府, 京都市
  • Year and Date: 2016-11-30
  • Int'l Joint Research
• [Presentation] Effect of low-load resistance training on muscle and gait function in resistance-untrained elderly individuals (2016)
  • Author(s): Kitada T, Ozaki H, Nakagata T, Yoshihara T, Natsume T, Ishihara Y, Ishibashi M, Machida S, Naito H
  • Organizer: 10th International Conference on Strength Training
  • Place of Presentation: 京都アバンティ, 京都府, 京都市
  • Year and Date: 2016-11-30
  • Int'l Joint Research
• [Presentation] Comparison of the effects of moderate-intensity walking and body mass-based resistance training on lower body muscle size and function in elderly individuals. (2016)
  • Author(s): Ozaki H, Nakagata T, Yoshihara T, Natsume T, Kitada T, Ishihara Y, Ishibashi M, Machida S, Naito H
  • Organizer: 10th International Conference on Strength Training
  • Place of Presentation: 京都アバンティ, 京都府, 京都市
  • Year and Date: 2016-11-30
  • Int'l Joint Research
• [Presentation] The characteristics of lower body muscle size and functions in elderly people at the early stage of locomotive syndrome (2016)
  • Author(s): Natsume T, Ozaki H, Nakagata H, Yoshihara T, Kitada T, Ishihara Y, Ishibashi M, Machida S, Naito H.
  • Organizer: 2nd Asia Conference for Frailty and Sarcopenia
  • Place of Presentation: 今池ガスビルホール, 愛知県, 名古屋市
  • Year and Date: 2016-11-04
  • Int'l Joint Research
• [Presentation] Effects of 5-month exercise intervention on the locomotive and cognitive function in elderly with locomotive syndrome (2016)
  • Author(s): Ishihara Y, Ozaki H, Nakagata T, Yoshihara T, Natsume T, Kitada T, Ishibashi M, Kuwata H, Miyazaki Y, Ikuta N, Machida S, Naito H
  • Organizer: 2nd Asia Conference for Frailty and Sarcopenia
  • Place of Presentation: 今池ガスビルホール, 愛知県, 名古屋市
  • Year and Date: 2016-11-04
  • Int'l Joint Research
• [Presentation] Lower body muscle size, strength, and quality in elderly Japanese men and women (2016)
  • Author(s): Ozaki H, Nakagata T, Yoshihara T, Natsume T, Kitada T, Ishihara Y, Ishibashi M, Machida S, Naito H
  • Organizer: 2nd Asia Conference for Frailty and Sarcopenia
  • Place of Presentation: 今池ガスビルホール, 愛知県, 名古屋市
  • Year and Date: 2016-11-04
  • Int'l Joint Research
• [Presentation] 筋電気刺激トレーニング刺激強度の違いが筋肥大及び筋力増加に及ぼす影響 (2016)
  • Author(s): 棗寿喜, 尾崎隼朗, 柿木亮, 小林裕幸, 内藤久士
  • Organizer: 第71回日本体力医学会大会
  • Place of Presentation: いわて県民情報交流センター, 岩手県, 盛岡市
  • Year and Date: 2016-09-23
• [Presentation] Muscle movements combined with blood flow restriction can induce muscle hypertrophy (2016)
  • Author(s): Natsume T, Ozaki H, Watanabe K, Kubota A, Naito H
  • Organizer: ACSM’s 63rd Annual meeting
  • Place of Presentation: Boston, USA
  • Year and Date: 2016-05-31
  • Int'l Joint Research
• [Presentation] Dose-dependent effects od whey peptide intake on mTOR signaling in human skeletal muscle (2016)
  • Author(s): Kakigi R, Yoshihata T, Natsume T, Ichinoseki-Sekine N, Kobayashi H, Naito H
  • Organizer: ACSM’s 63rd Annual meeting
  • Place of Presentation: Boston, USA
  • Year and Date: 2016-05-31
  • Int'l Joint Research
• [Presentation] Physical inactivity-induced histone modification in the rat soleus muscle (2016)
  • Author(s): Yoshihara T, Kakigi R, Tsuzuki T, Shuo-wen C, Natsume T, Takamine Y, Ichinoseki-Sekine N, Machida S, Sugiura T, Naito H
  • Organizer: ACSM’s 63rd Annual meeting
  • Place of Presentation: Boston, USA
  • Year and Date: 2016-05-31
  • Int'l Joint Research
• [Presentation] 中高齢者サイクリストの最大酸素摂取量と大腿前面筋厚はトレーニング強度に依存する (2016)
  • Author(s): 尾崎隼朗,北田友治, 棗寿喜, 町田修一, 内藤久士
  • Organizer: 第71回日本体力医学会大会
  • Place of Presentation: いわて県民情報交流センター, 岩手県, 盛岡市