Effect of exercise on brown adipose tissue and its therapeutic application to dyslipidemia

• Project/Area Number: 16K16572
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Sports science
• Research Institution: Department of Clinical Research, National Hospital Organization Kyoto Medical Center
• Principal Investigator: Nirengi Shinsuke 独立行政法人国立病院機構(京都医療センター臨床研究センター), 臨床研究企画運営部, 研究員 (70741156)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 褐色脂肪組織 / 近赤外線分光法 / 運動 / 自律神経 / 季節変動 / 褐色脂肪組織 (BAT) / カプシノイド / 生理学

Outline of Final Research Achievements

We have found and validated that near-infrared time-resolved spectroscopy can evaluate human brown adipose tissue (BAT) density. We have reported that BAT density is associated with age, body fat (subcutaneous and visceral), and outdoor temperature in a cross-sectional study. Several functional foods that increase BAT density have been also reported.
In animal studies, although the blood levels of triglyceride and cholesterol were increased by the high-fat diet, high-intensity intermittent exercise improved this condition. Furthermore, this exercise tends to increase the expression of uncoupling protein 1 in BAT. In recent years, it has been reported that the liquid factor derived from BAT may cross-talk with other tissues. Therefore, it is necessary to study in detail the relation between BAT and lipid metabolism.

Academic Significance and Societal Importance of the Research Achievements

これまで評価困難であったヒトBATの簡易な評価法の考案および妥当性の検証を行った。BATに影響する因子を探索することができた。運動によりBAT活性が増加する可能性を見出すことができた。これらの結果は、今後、BATと生活習慣病との関連の詳細な検討や、生活習慣を是正するための基礎データとなりうる。

Research Products

• [Journal Article] Nonalcoholic Fatty Liver Disease in University Rugby Football Players. (2018)
  • Author(s): Nirengi S, Fujibayashi M, Furuno S, Uchibe A, Kawase Y, Sukino S, Kawaguchi Y, Minato S, Kotani K, Sakane N.
  • Journal Title: Front Endocrinol (Lausanne). Volume: 9 Pages: 341-341
  • DOI: 10.3389/fendo.2018.00341
  • Peer Reviewed / Open Access
• [Journal Article] Brown adipose tissue density measured by near-infrared time-resolved spectroscopy in Japanese, across a wide age range (2018)
  • Author(s): Fuse Sayuri、Nirengi Shinsuke、Amagasa Shiho、Homma Toshiyuki、Kime Ryotaro、Endo Tasuki、Sakane Naoki、Matsushita Mami、Saito Masayuki、Yoneshiro Takeshi、Kurosawa Yuko、Hamaoka Takafumi
  • Journal Title: Journal of Biomedical Optics Volume: 23 Issue: 06 Pages: 1-1
  • DOI: 10.1117/1.jbo.23.6.065002
  • Peer Reviewed / Open Access
• [Journal Article] Effects of a Low Advanced Glycation End Products Diet on Insulin Levels: The Feasibility of a Crossover Comparison Test. (2018)
  • Author(s): Sukino S, Nirengi S, Kawaguchi Y, Kotani K, Tsuzaki K, Okada H, Suganuma A, Sakane N.
  • Journal Title: Journal of clinical medicine research Volume: 10 Issue: 5 Pages: 405-410
  • DOI: 10.14740/jocmr3301w
  • Peer Reviewed / Open Access
• [Journal Article] Safety of the batteries and power units used in insulin pumps: A pilot cross-sectional study by the Association for the Study of Innovative Diabetes Treatment in Japan. (2018)
  • Author(s): Murata T, Nirengi S, Sakane N, Kuroda A, Hirota Y, Matsuhisa M, Namba M, Kobayashi T; Association for the Study of Innovative Diabetes Treatment in Japan (ASINDTJ).
  • Journal Title: J Diabetes Investig. Volume: 9 Issue: 4 Pages: 903-907
  • DOI: 10.1111/jdi.12765
  • Peer Reviewed / Open Access
• [Journal Article] High-Density Lipoprotein Subclasses and Mild Cognitive Impairment: Study of Outcome and aPolipoproteins in Dementia (STOP-Dementia)1. (2018)
  • Author(s): Ohtani R, Nirengi S, Nakamura M, Murase N, Sainouchi M, Kuwata Y, Takata M, Masuda Y, Kotani K, Sakane N.
  • Journal Title: J Alzheimers Dis. Volume: 66 Issue: 1 Pages: 289-296
  • DOI: 10.3233/jad-180135
  • Peer Reviewed / Open Access
• [Journal Article] Prevalence and Risk Factors of Elevated Liver Enzymes in Japanese Women With Polycystic Ovary Syndrome. (2018)
  • Author(s): Minato S, Sakane N, Kotani K, Nirengi S, Hayashi I, Suganuma A, Yamaguchi K, Takakura K, Nagai N.
  • Journal Title: J Clin Med Res. Volume: 10 Issue: 12 Pages: 904-910
  • DOI: 10.14740/jocmr3639
  • Peer Reviewed / Open Access
• [Journal Article] Seasonal differences in brown adipose tissue density and pulse rate variability in a thermoneutral environment (2018)
  • Author(s): Nirengi S, Sakane N, Amagasa S, Wakui S, Homma T, Kurosawa Y, Hamaoka T.
  • Journal Title: Journal of Physiological Anthropology Volume: 37 Issue: 1 Pages: 6-6
  • DOI: 10.1186/s40101-018-0166-x
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 脂質異常症における療養指導プログラム：脂質パラメーターの前後比較 (2018)
  • Author(s): 鋤納 心, 河口 八重子, 二連木 晋輔, 小谷 和彦, 菅沼 彰子, 坂根 直樹
  • Journal Title: 日本糖尿病学会誌 Volume: -
  • Peer Reviewed
• [Journal Article] Determinants of Glycated Hemoglobin in Subjects With Impaired Glucose Tolerance: Subanalysis of the Japan Diabetes Prevention Program. (2017)
  • Author(s): Sakane N, Sato J, Tsushita K, Tsujii S, Kotani K, Tominaga M, Kawazu S, Sato Y, Usui T, Kamae I, Yoshida T, Kiyohara Y, Sato S, Tsuzaki K, Nirengi S, Takahashi K, Kuzuya H, Group JR.
  • Journal Title: J Clin Med Res. Volume: 7 Issue: 4 Pages: 360-365
  • DOI: 10.14740/jocmr2928w
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Mushroom intake and advanced glycation end products in the skin among community-dwelling elderly subjects: preliminary data (2017)
  • Author(s): 河口八重子
  • Journal Title: Joutnal of Biomedicine Volume: 2 Pages: 8-11
  • DOI: 10.7150/jbm.17587
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] 日本人妊婦における妊娠前の体格、体型認識と妊娠中の体重増加との関連 (2017)
  • Author(s): 林 育代, 鈴木 麻希, 能勢 陽子, 湊 聡美, 住友 文, 二連木 晋輔, 津崎 こころ, 坂根 直樹, 住友 理浩, 髙倉 賢二, 永井 成美
  • Journal Title: 肥満研究 Volume: 23 Pages: 233-240
  • NAID: 40021545967
  • Peer Reviewed
• [Journal Article] Diagnostic accuracy of the anti-glutamic acid decarboxylase antibody in type 1 diabetes mellitus: Comparison between radioimmunoassay and enzyme-linked immunosorbent assay. (2016)
  • Author(s): Murata T, Tsuzaki K, Nirengi S, Watanabe T, Mizutani Y, Okada H, Tsukamoto M, Odori S, Nakagawachi R, Kawaguchi Y, Yoshioka F, Yamada K, Shimatsu A, Kotani K, Satoh-Asahara N, Sakane N.
  • Journal Title: J Diabetes Investig Volume: - Issue: 4 Pages: 475-479
  • DOI: 10.1111/jdi.12594
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Near-infrared time resolved spectroscopy assessment of changes in human brown adipose tissue density during repeated capsiate intake and intake cessation (2016)
  • Author(s): Nirengi S, Homma T, Inoue N, Sato H, Yoneshiro T, Tsuzaki K, Saito M, Sakane N, Kurosawa Y, Hamaoka T
  • Journal Title: J Biomed Opt Volume: 21 Issue: 9 Pages: 091305-091305
  • DOI: 10.1117/1.jbo.21.9.091305
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Daily ingestion of catechin-rich beverage increases brown adipose tissue density and decreases extramyocellular lipids in healthy young women (2016)
  • Author(s): Nirengi S, Amagasa S, Homma T, Yoneshiro T, Matsumiya S, Kurosawa Y, Sakane N, Ebi K, Saito M, Hamaoka T
  • Journal Title: SplingerPlus Volume: 5 Issue: 1 Pages: 1363-1363
  • DOI: 10.1186/s40064-016-3029-0
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] ACTN3 GENE R577X POLYMORPHISM ASSOCIATED WITH HIGH-DENSITY LIPOPROTEIN CHOLESTEROL AND ADIPONECTIN IN RUGBY PLAYERS. (2016)
  • Author(s): Shinsuke Nirengi, Mami Fujibayashi, Kokoro Tsuzaki Sachiko Furuno, Akihiko Uchibe, Yasuharu Kawase, Kazuhiko Kotani, Naoki Sakane
  • Journal Title: ENDOCRINE PRACTICE. Volume: 22(7) Issue: 7 Pages: 786-90
  • DOI: 10.4158/ep15963.or
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Effects of the Drop-set and Reverse Drop-set Methods on the Muscle Activity and Intramuscular Oxygenation of the Triceps Brachii among Trained and Un-trained Individuals (2016)
  • Author(s): Goto M, Nirengi S, Kurosawa Y, Nagano A, Hamaoka T.
  • Journal Title: Journal of Sports Science and Medicine Volume: 15 Pages: 562-568
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] ヒト褐色脂肪組織の活性評価に関する最新研究とアスリートへの応用可能性 (2019)
  • Author(s): 二連木晋輔
  • Organizer: 2018年度第1回日本生理人類学会体温調節研究部会
  • Invited
• [Presentation] Non-invasive evaluation of human brown adipose tissue using infrared thermography (2018)
  • Author(s): Nirengi Shinsuke, Wakabayashi Hitoshi, Matsushita Mami, Domichi Masayuki, Suzuki Shinichi, Sukino Shin, Kawaguchi Yaeko, Hashimoto Takeshi, Saito Masayuki, Sakane Naoki.
  • Organizer: 12th IDF-WPR Congress & 10th ASSD Scientific Meeting
  • Int'l Joint Research
• [Presentation] Development of modified sensory-based food education program for 2yrs. and 3yrs children at the nursery school in Japan (2018)
  • Author(s): Kawaguchi Yaeko, Somei Jyunichiro, Suganuma Akiko, Nirengi Shinsuke, Sakane Naoki.
  • Organizer: 12th IDF-WPR Congress 2018 & 10th ASSD Scientific Meeting
  • Int'l Joint Research
• [Presentation] Effectiveness of a pharmacist-delivered smoking cessation program in community pharmacy (FINE project):A feasibility study in Japan (2018)
  • Author(s): Domichi Masayuki, Onda Mitsuko, Kuwanoe Takashi, Hashimoto Atsunori, Horiguchi Michiko, Abe Keiko, Suganuma Akiko, Nirengi Shinsuke, Sakane Naoki.
  • Organizer: 12th IDF-WPR Congress 2018 & 10th ASSD Scientific Meeting
  • Int'l Joint Research
• [Presentation] Supraclavicular tissue total hemoglobin determined by near-infrared time resolved spectroscopy as indicators of brown adipose tissue characteristics in humans (2018)
  • Author(s): Hamaoka Takafumi, Nirengi Shinsuke, Fuse Sayuri, Amagasa Shiho, Homma Toshiyuki, Kime Ryotaro, Kuroiwa Miyuki, Endo Tasuki, Sakane Naoki, Matsushita Mami, Saito Masayuki, Yoneshiro Takeshi, Kurosawa Yuko
  • Organizer: Role of Brown adipose tissue in human health
  • Int'l Joint Research
• [Presentation] Comparison of apolipoprotein A1 and carotid artery ultrasonography findings among stroke subtypes and controls (2018)
  • Author(s): Ohtani Ryo, Nirengi Sshinsuke, Nakamura Michikazu, Togawa Jumpei, Kuwata Yasuhiro, Takata Masaki, Kiyoaki Takeda, Kotani Kazuhiko, Sakane Naoki.
  • Organizer: World Stroke Congress 2018
  • Int'l Joint Research
• [Presentation] 赤外線サーモグラフィ法による非侵襲的なヒト褐色脂肪組織活性の評価法の開発 (2018)
  • Author(s): 二連木晋輔, 若林斉, 松下真美, 同道正行, 鈴木新一, 橋本健, 鋤納心, 菅沼彰子, 河口八重子, 斉藤昌之, 坂根直樹
  • Organizer: 第39回 日本肥満学会
• [Presentation] 中年期における健常者および肥満者の褐色脂肪組織密度の比較 (2018)
  • Author(s): 布施沙由理, 二連木晋輔, 天笠志保, 黒岩美幸, 遠藤祐輝, 木目良太郎, 黒澤裕子, 浜岡隆文
  • Organizer: 第73回 日本体力医学会
• [Presentation] 大学ラグビー選手におけるヘプシジンと肥満度との関連 (2018)
  • Author(s): 二連木晋輔, 藤林真美, 古野幸子, 同道正行, 鋤納心, 河口八重子, 菅沼彰子, 河瀬泰治, 坂根直樹
  • Organizer: 第73回 日本体力医学会
• [Presentation] ラグビー選手におけるACEおよびACTN3遺伝子多型と損傷経験との関連 (2018)
  • Author(s): 藤林真美, 二連木晋輔, 古野幸子, 河瀬泰治, 坂根直樹
  • Organizer: 第73回 日本体力医学会
• [Presentation] The impact of lifestyle intervention on stage of change toward healthy eating and development of diabetes in prediabetic individuals in precontemplation stage (2018)
  • Author(s): Sakane N, Kotani K, Nirengi S, Takahashi K, Sano Y, Okazaki K, Sato J, Suzuki S, Morita S, Oshima Y, Izumi K, Kato M, Ishizuka N, Noda M, Kuzuya H.
  • Organizer: American Diabetes Association's 78th Scientific Sessions
  • Int'l Joint Research
• [Presentation] Human brown adipose tissue density evaluated by near-infrared time-resolved spectroscopy (2018)
  • Author(s): Hamaoka Takafumi, Nirengi Sshinsuke, Fuse Sayuri, Amagasa Shiho, Homma Toshiyuki, Kime Ryotaro, Endo Tasuki, Sakane Naoki, Matsushita Mami, Saito Masayuki, Yoneshiro Takeshi, Kurosawa Yuko.
  • Organizer: The Second Britton Chance International Symposium on Metabolic Imaging and Spectroscopy 2018
  • Int'l Joint Research
• [Presentation] 個人毎の最適なエネルギー摂取やお勧めの栄養素バランスを提示する糖尿病発症予測システムの開発：機序計算モデルを用いて (2018)
  • Author(s): 坂根直樹, Chen Julia, 深澤桃子, Chen Qian, Burns Samuel, クマール圭, 二連木晋輔, 高橋かおる, 菅沼彰子, 葛谷英嗣, Dwivedi Gaurav
  • Organizer: 第61回 日本糖尿病学会
  • Invited
• [Presentation] 生活習慣病予防のためのシミュレーションモデルの開発;食事療法の最適化 (2018)
  • Author(s): 坂根直樹, CHEN Julia, 深澤桃子, CHEN Qian, BURNS Samuel, クマール圭, 二連木晋輔, 高橋かおる, 菅沼彰子, 葛谷英嗣, DWIVEDI Gaurav
  • Organizer: 産業衛生学会
  • Invited
• [Presentation] 近赤外時間分解分光法によって評価した幅広い年代における褐色脂肪組織密度とその関連要因 (2017)
  • Author(s): 布施沙由理, 黒澤裕子, 木目良太郎, 遠藤祐輝, 安生幹子, 木目綾佳, 天笠志保, 井上茂, 二連木晋輔, 坂根直樹, 米代武司, 斉藤昌之, 浜岡隆文.
  • Organizer: 第 169 回日本体力医学会関東地方会
  • Place of Presentation: 東京
  • Year and Date: 2017-03-18
• [Presentation] 寒冷誘導熱産生とヒト褐色脂肪組織および交感神経性超低周波領域成分（VLF）との関連 (2017)
  • Author(s): 二連木晋輔 坂根直樹 天笠志保 本間俊行 布施沙由理 黒澤裕子 浜岡隆文
  • Organizer: 第38回日本肥満学会
• [Presentation] 近赤外時間分解分光法によって評価した幅広い年代における褐色脂肪組織密度 (2017)
  • Author(s): 布施沙由理 二連木晋輔 天笠志保 本間俊行 木目良太郎 遠藤祐輝 坂根直樹 松下真美 斉藤昌之 米代武司 黒澤裕子 浜岡隆文
  • Organizer: 第171回日本体力医学会関東地方会
• [Presentation] 大学ラグビー選手におけるACTN3 R577Xのポジション別比較 (2017)
  • Author(s): 二連木晋輔 藤林真美 古野幸子 内部昭彦 河瀬泰治 坂根直樹
  • Organizer: 第72回日本体力医学会
• [Presentation] 近赤外線時間分解分光法によって評価した褐色脂肪組織密度と生活環境との関係 (2016)
  • Author(s): 布施沙由理, 二連木晋輔, 本間俊行, 木目良太郎, 黒澤裕子, 浜岡隆文.
  • Organizer: 第23回 医用近赤研究会
  • Place of Presentation: 東京
  • Year and Date: 2016-10-29
• [Presentation] 近赤外線時間分解分光法によって評価した褐色脂肪組織密度と関連要因の検討 (2016)
  • Author(s): 布施沙由理, 二連木晋輔, 本間俊行, 坂根直樹, 米代武司, 斉藤昌之, 浜岡隆文.
  • Organizer: 第37回 日本肥満学会
  • Place of Presentation: 東京
  • Year and Date: 2016-10-07
• [Presentation] ヒトにおける体脂肪量と褐色脂肪組織との関係：近赤外線時間分解分光法による評価 (2016)
  • Author(s): 布施沙由理, 二連木晋輔, 天笠志保, 本間俊行, 木目良太郎, 村瀬訓生, 黒澤裕子, 浜岡隆文.
  • Organizer: 第71回 日本体力医学会
  • Place of Presentation: 岩手
  • Year and Date: 2016-09-23
• [Presentation] The association between body fatness and brown adipose tissue in humans: the evaluation by near-infrared time-resolved spectroscopy. (2016)
  • Author(s): Fuse S, Nirengi S, Homma T, Kime R, Murase N, Kurosawa Y, Hamaoka T.
  • Organizer: 13th International Congress on Obesity (ICO)
  • Place of Presentation: Vancouver
  • Year and Date: 2016-05-01
  • Int'l Joint Research
• [Book] いろいろな脂肪 (2017)
  • Author(s): 二連木晋輔
  • Total Pages: 4
  • Publisher: 糖尿病ケア
• [Book] 糖尿病学2017 (2017)
  • Author(s): 浜岡隆文 二連木晋輔 坂根直樹 米代武司 斎藤昌之
  • Total Pages: 9
  • Publisher: 医学書出版
  • ISBN: 4787823019