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Studies on the physiological role of ascorbic acid in SOD1/Akr1a double knockout mice with increased oxidative stress

Research Project

Project/Area Number 16K16592
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Applied health science
Research InstitutionYamagata University

Principal Investigator

Homma Takujiro  山形大学, 大学院医学系研究科, 助教 (70743566)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords酸化ストレス / スーパーオキシド / ビタミンC / 小胞体ストレス / オートファジー
Outline of Final Research Achievements

To elucidate the physiological role of superoxide and ascorbic acid in vivo, we have established the SOD1/Akr1a double knockout (DKO) mice.The DKO mice had serious phenotype with heightened oxidative stress. After AsA withdrawal, all DKO mice died within two weeks. The AsA withdrawal for a week significantly increased plasma ALT levels but had no effect on cardiac function in the DKO mice.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (21 results)

All 2017 2016

All Journal Article (12 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 12 results,  Open Access: 3 results,  Acknowledgement Compliant: 2 results) Presentation (7 results) (of which Int'l Joint Research: 2 results) Book (2 results)

  • [Journal Article] The viability of primary hepatocytes in maintained under a low cysteine-glutathione redox state with a marked elevation in ophthalmic acid production.2017

    • Author(s)
      Lee, J., Kang, E. S., Kobayashi, S., Homma, T., Sato, H., Seo, H. G., and Fujii, J.
    • Journal Title

      Exp. Cell Res.

      Volume: 361 Issue: 1 Pages: 178-191

    • DOI

      10.1016/j.yexcr.2017.10.017

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Decreased reproductive performance in xCT-knockout male mice.2017

    • Author(s)
      Hamashima, S., Homma, T., Kobayashi, S., Ishii, N., Kurahashi, T., Watanabe, R., Kimura, N., Sato, H., and Fujii, J.
    • Journal Title

      Free Radic. Res.

      Volume: 51 Issue: 9-10 Pages: 851-860

    • DOI

      10.1080/10715762.2017.1388504

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Mice in the early stage of liver steatosis caused by a high fat diet are resistant to thioacetamide-induced hepatotoxicity and oxidative stress2017

    • Author(s)
      Lee Jaeyong、Homma Takujiro、Fujii Junichi
    • Journal Title

      Toxicol Lett

      Volume: 277 Pages: 92-103

    • DOI

      10.1016/j.toxlet.2017.06.005

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] xCT deficiency aggravates acetaminophen-induced hepatotoxicity under inhibition of the transsulfuration pathway2017

    • Author(s)
      Eun Sil Kang, Jaeyong Lee, Takujiro Homma, Toshihiro Kurahashi, Sho Kobayashi, Atsunori Nabeshima, Sohsuke Yamada, Han GeuK Seo, Satoshi Miyata, Hideyo Sato, Junichi Fujii
    • Journal Title

      Free Radic Res

      Volume: 51 Issue: 1 Pages: 80-90

    • DOI

      10.1080/10715762.2017.1282157

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Oxidative stress caused by a SOD1 deficiency ameliorates thioacetamide-triggered cell death via CYP2E1 inhibition but stimulates liver steatosis.2017

    • Author(s)
      Takaya Shirato, Takujiro Homma, Jaeyong Lee, Toshihiro Kurahashi, Junichi Fujii
    • Journal Title

      Archives of Toxicology

      Volume: 91 Issue: 3 Pages: 1319-1333

    • DOI

      10.1007/s00204-016-1785-9

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Heightened aggressive behavior in mice deficient in aldo-keto reductase 1a (Akr1a).2017

    • Author(s)
      Takujiro Homma, Ryusuke Akihara, Satoshi Okano, Mototada Shichiri, Yasukazu Yoshida, Ken-ichi Yamada, Satoshi Miyata, Osamu Nakajima, Junichi Fujii
    • Journal Title

      Behavioural Brain Research

      Volume: 319 Pages: 219-224

    • DOI

      10.1016/j.bbr.2016.11.038

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] A high fat diet temporarily renders Sod1-deficient mice resistant to an oxidative insult2017

    • Author(s)
      Junitsu Ito, Naoki Ishii, Ryusuke Akihara, Jaeyong Lee, Toshihiro Kurahashi, Takujiro Homma, Ryo Kawasaki, Junichi Fujii
    • Journal Title

      J Nutr Biochem

      Volume: 40 Pages: 44-52

    • DOI

      10.1016/j.jnutbio.2016.10.018

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Heat stress promotes the down-regulation of IRE1α in cells: An atypical modulation of the UPR pathway2016

    • Author(s)
      Takujiro Homma, Junichi Fujii
    • Journal Title

      Exp Cell Res

      Volume: 349 Issue: 1 Pages: 128-138

    • DOI

      10.1016/j.yexcr.2016.10.006

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Ablation of aldehyde reductase aggravates carbon tetrachloride-induced acute hepatic injury involving oxidative stress and endoplasmic reticulum stress2016

    • Author(s)
      Ryusuke Akihara, Takujiro Homma, Jaeyong Lee, Ken-ichi Yamada, Satoshi Miyata, Junichi Fujii
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 478 Issue: 2 Pages: 765-771

    • DOI

      10.1016/j.bbrc.2016.08.022

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Ascorbic acid prevents acetaminophen-induced hepatotoxicity in mice by ameliorating glutathione recovery and autophagy.2016

    • Author(s)
      Kurahashi T, Lee J, Nabeshima A, Homma T, Kang ES, Saito Y, Yamada S, Nakayama T, Yamada K, Miyata S, Fujii J.
    • Journal Title

      Arch Biochem Biophys

      Volume: 604 Pages: 36-46

    • DOI

      10.1016/j.abb.2016.06.004

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Trichloroethylene exposure aggravates behavioral abnormalities in mice that are deficient in superoxide dismutase.2016

    • Author(s)
      Otsuki N, Homma T, Fujiwara H, Kaneko K, Hozumi Y, Shichiri M, Takashima M, Ito J, Konno T, Kurahashi T, Yoshida Y, Goto K, Fujii S, Fujii J.
    • Journal Title

      Regul Toxicol Pharmacol

      Volume: 79 Pages: 83-90

    • DOI

      10.1016/j.yrtph.2016.05.007

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] The SOD1 transgene expressed in erythroid cells alleviates fatal phenotype in congenic NZB/NZW-F1 mice.2016

    • Author(s)
      Otsuki N, Konno T, Kurahashi T, Suzuki S, Lee J, Okada F, Iuchi Y, Homma T, Fujii J.
    • Journal Title

      Free Radic Res

      Volume: 50 Issue: 7 Pages: 793-800

    • DOI

      10.1080/10715762.2016.1178388

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] Heterozygous SOD1 deficiency causes male infertility by triggering autoimmune response in mice with the NZW background.2017

    • Author(s)
      Takujiro Homma, Naoki Ishii, Sho Kobayashi, and Junichi Fujii.
    • Organizer
      8th Joint Meeting of Society for Free Radical Research Australasia and Japan with International Symposium on Coenzyme Q10
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] シスチントランスポーター・xCT欠損マウスは雄性生殖能が低下する2017

    • Author(s)
      本間拓二郎、浜島真司、小林翔、倉橋敏裕、渡辺連、木村直子、佐藤英世、藤井順逸
    • Organizer
      2017年度生命科学系学会合同年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] シスチントランスポーター・xCTの男性生殖能における役割の検討2017

    • Author(s)
      本間拓二郎、浜島真司、小林翔、倉橋敏裕、渡辺連、木村直子、佐藤英世、藤井順逸
    • Organizer
      第70回日本酸化ストレス学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] SOD1-knockout mice are resistant to lethal effects of thioacetamide-induced hepatotoxicity.2016

    • Author(s)
      Takujiro Homma, Takaya Shirato, Jaeyong Lee, Toshihiro Kurahashi, Junichi Fujii.
    • Organizer
      The 23rd Annual Meeting, a joint meeting with the Society for Free Radical Research International (SfRBM/SFRRI 2016)
    • Place of Presentation
      San Francisco, USA
    • Year and Date
      2016-11-16
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 熱ストレス障害に対する小胞体ストレス応答の解析;熱ストレスはIRE1発現を減少させる2016

    • Author(s)
      本間拓二郎、藤井順逸
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      仙台国際センター会議棟(仙台市)
    • Year and Date
      2016-09-25
    • Related Report
      2016 Research-status Report
  • [Presentation] SOD1欠損による酸化ストレスはシトクロムP450活性を抑制することで薬剤誘発性肝障害を軽減する2016

    • Author(s)
      本間拓二郎、白土貴也、李在勇、倉橋敏裕、藤井順逸
    • Organizer
      第69回日本酸化ストレス学会学術集会
    • Place of Presentation
      仙台国際センター会議棟(仙台市)
    • Year and Date
      2016-08-30
    • Related Report
      2016 Research-status Report
  • [Presentation] SOD1欠損による酸化ストレスはシトクロムP450活性を抑制することで薬剤誘発性肝障害を軽減する2016

    • Author(s)
      本間拓二郎、白土貴也、李在勇、倉橋敏裕、藤井順逸
    • Organizer
      日本生化学会 東北支部 第82回例会・シンポジウム
    • Place of Presentation
      弘前大学医学部(弘前市)
    • Year and Date
      2016-05-21
    • Related Report
      2016 Research-status Report
  • [Book] Superoxide Dismutase (SOD): Sources, Therapeutic Uses and Health Benefits.2016

    • Author(s)
      Takujiro Homma, Junichi Fujii
    • Total Pages
      197
    • Publisher
      Nova Science Publishers, Inc
    • Related Report
      2016 Research-status Report
  • [Book] Ascorbic Acid: Properties, Synthesis and Applications.2016

    • Author(s)
      Junichi Fujii, Takujiro Homma, Sho Kobayashi
    • Total Pages
      285
    • Publisher
      Nova Science Publishers, Inc
    • Related Report
      2016 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2019-03-29  

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