| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
In this study, we succeeded in developing a rapid method for detecting isomerization of aspartic acid (Asp) residue and glutamic acid (Glu) residue. Aromatic amino acids such as tryptophan and tyrosine suppressed the isomerization reaction of Asp residues in peptides by ultraviolet. It was considered that the energy absorbed by the aromatic amino acids were not transferred to the isomerization reaction of Asp and was used for decomposition to protect the peptide from isomerization. On the other hand, it was also suggested that pH change and coexistence of reducing sugars promote isomerization. It was suggested that UV irradiation accelerated the isomerization of Asp indirectly.
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