Implantable device for measuring cerebral blood flow, neural activities and animal behaviors

• Project/Area Number: 16K16652
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Brain biometrics
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: Haruta Makito 奈良先端科学技術大学院大学, 先端科学技術研究科, 助教 (40733663)
• Research Collaborator: Kurauchi Yuki
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: CMOSイメージセンサ / 脳機能イメージング / 脳血流計測 / 生体埋植デバイス / 自由行動実験 / 脳血管障害 / 長期計測 / 蛍光計測 / CMOSイメージセンサー / 脳虚血 / CMOSイメージングデバイス / 脳血流 / 脳機能 / LED / 多機能イメージング / 生体埋め込みデバイス / 電子デバイス・機器 / 脳・神経 / 脳神経疾患 / 神経科学

Outline of Final Research Achievements

For understanding mechanism of the cerebrovascular disease, we have observed brain activities and blood-flow in a small animal. We have developed a chronic blood-flow imaging device for long-time observing brain activity and blood-flow simultaneously. This device has a CMOS image sensor mounted on a small electronic substrate, a light source with green LEDs. In addition, we have improved an implantation method of the device. We make a special chronic window on a small animal head. The new method allowed observation of the brain surface for more than one month in an animal. Our chronic blood-flow imaging device contributes to development of therapy of the cerebrovascular disease.

Academic Significance and Societal Importance of the Research Achievements

脳機能低下時や脳機能回復過程における血流変化が及ぼす影響を解明するために、自由行動実験で使用可能な超小型脳機能測定デバイスの開発を行った。特に、脳機能回復過程を詳細に調べるためには、1週間以上の長期に渡って安定して脳表観察を実現する必要がある。そのため、長期間の脳表脳機能測定を目的とし、新手法を用いた超小型脳機能測定デバイスの開発を行った。本手法では1ヵ月以上の長期観測を実現した。これにより、脳障害前後および回復過程における脳表観測を実現できるため、脳機能回復メカニズムの解明が期待できる。また、脳機能回復を目指した治療法開発や新薬開発において本手法を用いることで開発を推進することが可能になる。

Research Products

• [Journal Article] Chronic brain blood-flow imaging device for a behavioral experiment using mice (2019)
  • Author(s): Haruta Makito、Kurauchi Yuki、Ohsawa Masahiro、Inami Chihiro、Tanaka Risako、Sugie Kenji、Kimura Ayaka、Ohta Yasumi、Noda Toshihiko、Sasagawa Kiyotaka、Tokuda Takashi、Katsuki Hiroshi、Ohta Jun
  • Journal Title: Biomedical Optics Express Volume: 10 Issue: 4 Pages: 1557-1557
  • DOI: 10.1364/boe.10.001557
  • Peer Reviewed / Open Access
• [Journal Article] Propranolol prevents cerebral blood flow changes and pain-related behaviors in migraine model mice (2019)
  • Author(s): Kurauchi Yuki、Haruta Makito、Tanaka Risako、Sasagawa Kiyotaka、Ohta Jun、Hisatsune Akinori、Seki Takahiro、Katsuki Hiroshi
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 508 Issue: 2 Pages: 445-450
  • DOI: 10.1016/j.bbrc.2018.11.173
  • Peer Reviewed / Open Access
• [Journal Article] Implantable optogenetic device with CMOS IC technology for simultaneous optical measurement and stimulation (2017)
  • Author(s): Makito Haruta,Naoya Kamiyama,Shun Nakajima,Mayumi Motoyama,Mamiko Kawahara Yasumi Ohta,Atsushi Yamasaki,Hiroaki Takehara,Toshihiko Noda,Kiyotaka Sasagawa,Yasuyuki Ishikawa,Takashi Tokuda,Hitoshi Hashimoto,Jun Ohta
  • Journal Title: Japanese Journal of Applied Physics Volume: 56 Issue: 5 Pages: 057001-057001
  • DOI: 10.7567/jjap.56.057001
  • NAID: 210000147741
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] A chronic blood-flow imaging device for a small animal's brain in a behavior experiment (2018)
  • Author(s): Makito Haruta, Yuki Kurauchi, Ayaka Kimura, Yasumi Ohta, Toshihiko Noda, Kiyotaka Sasagawa, Takashi Tokuda, Hiroshi Katsuki, Jun Ohta
  • Organizer: NEUROSCIENCE 2018
  • Int'l Joint Research
• [Presentation] 小型動物用超小型イメージングデバイスによる脳疾患モデルマウスの脳血流・脳機能同時計測 (2018)
  • Author(s): 春田牧人
  • Organizer: 日本薬学会大138年会
  • Invited
• [Presentation] 細胞観察用小型蛍光イメージングシステムの開発 (2017)
  • Author(s): 春田牧人
  • Organizer: NAIST異分野融合ワークショップ「刺激を与えて細胞を制御する」
  • Place of Presentation: 奈良先端科学技術大学院大学(奈良県生駒市)
  • Year and Date: 2017-03-06
  • Invited
• [Presentation] A miniaturized imaging device for detecting biological signals in a brain (2017)
  • Author(s): Makino Haruta
  • Organizer: 2017 NAIST / NCTU Joint Workshop on Retinal Implant Technologies and Materials
  • Int'l Joint Research
• [Presentation] A portable cell fluorescence and bright imaging system (2017)
  • Author(s): Makito Haruta, Kenta Nakamoto, Toshihiko Noda, Kiyotaka Sasagawa, Takashi Tokuda, Jun Ohta
  • Organizer: Neuroscience2017
• [Presentation] An implantable imaging device for observing the recovery process from cerebrovascular disease in small animal brains (2016)
  • Author(s): Makito Haruta, Yoshinori Sunaga, Toshihiko Noda, Kiyotaka Sasagawa, Takashi Tokuda, and Jun Ohta
  • Organizer: 2016 GLOBAL RESEARCH EFFORTS ON ENERGY AND NANOMATERIALS
  • Place of Presentation: Taipei(Taiwan)
  • Year and Date: 2016-12-22
  • Int'l Joint Research / Invited
• [Presentation] A portable cell imaging system with dual imaging functionality (2016)
  • Author(s): Makito Haruta, Anek Wuthayavanich, Kazutaka Osawa, Toshihiko Noda, Kiyotaka Sasagawa, Takashi Tokuda, and Jun Ohta
  • Organizer: International Conference on BioSensors, BioElectronics, BioMedical Devices, BioMEMS/NEMS & Applicatio
  • Place of Presentation: Gold Coast(Australia)
  • Year and Date: 2016-12-14
  • Int'l Joint Research