Development of glycan-immobilized field effect transistor based biosensors toward the detection of mutated influenza viruses

• Project/Area Number: 16K17498
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Nanobioscience
• Research Institution: Waseda University
• Principal Investigator: Hideshima Sho 早稲田大学, ナノ・ライフ創新研究機構, 次席研究員(研究院講師) (10580433)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 電界効果トランジスタバイオセンサ / インフルエンザウイルス / 糖鎖 / バイオセンシング / ナノバイオ界面 / 分子間相互作用 / 糖鎖クラスター効果

Outline of Final Research Achievements

In the project, a glycan-immobilized field effect transistor (FET) based biosensor toward the detection of mutated influenza viruses has been developed. The glycan-immobilized FET biosensors, which were modified with Siaα2,6’Gal and Siaα2,3’Gal, successfully detected human and avian influenza viral particles, respectively, suggesting that mutant influenza viruses, which has their recognition ability both to Siaα2,6’Gal and Siaα2,3’Gal, could be rapidly identified by the biosensors.

Research Products

• [Journal Article] Label-free detection of allergens in food via surfactant-induced signal amplification using a field effect transistor-based biosensor (2018)
  • Author(s): Hideshima Sho、Saito Mai、Fujita Keisuke、Harada Yoshitaka、Tsuna Mika、Sekiguchi Satoshi、Kuroiwa Shigeki、Nakanishi Takuya、Osaka Tetsuya
  • Journal Title: Sensors and Actuators B: Chemical Volume: 254 Pages: 1011-1016
  • DOI: 10.1016/j.snb.2017.07.187
  • Peer Reviewed / Open Access
• [Journal Article] Effect of human serum on the electrical detection of amyloid-β fibrils in biological environments using azo-dye immobilized field effect transistor (FET) biosensor (2018)
  • Author(s): Hideshima Sho、Wustoni Shofarul、Kobayashi Masumi、Hayashi Hiroki、Kuroiwa Shigeki、Nakanishi Takuya、Osaka Tetsuya
  • Journal Title: Sensing and Bio-Sensing Research Volume: 17 Pages: 25-29
  • DOI: 10.1016/j.sbsr.2018.01.003
  • Peer Reviewed / Open Access
• [Journal Article] Review of Physiological Balance Sensing in an Unobtrusive Manner (2017)
  • Author(s): OHASHI KEISHI、KUROIWA SHIGEKI、HIDESHIMA SHO、NAKANISHI TAKUYA、OSAKA TETSUYA
  • Journal Title: Electronics and Communications in Japan Volume: 100 Issue: 9 Pages: 50-55
  • DOI: 10.1002/ecj.11983
  • NAID: 210000172328
  • Peer Reviewed
• [Presentation] 健康モニタリングに資する半導体バイオセンサ技術 (2017)
  • Author(s): 秀島翔
  • Organizer: 電気化学会第84回大会
  • Place of Presentation: 東京
  • Year and Date: 2017-03-25
  • Invited
• [Presentation] 糖鎖固定化電界効果トランジスタバイオセンサによるインフルエンザウイルス粒子の高感度検出 (2017)
  • Author(s): 林宏樹、秀島翔、比能洋、西村紳一郎、迫田義博、黒岩繁樹、中西卓也、門間聰之、逢坂哲彌
  • Organizer: Conference for Biosignal and Medicine (CBSM) 2016
• [Presentation] 溶液中保存の安定性向上を目指した電界効果トランジスタバイオセンサゲート絶縁膜への酸化グラフェン被覆 (2017)
  • Author(s): 竹内亮、秀島翔、黒岩繁樹、中西卓也、門間聰之、逢坂哲彌
  • Organizer: 第62回化学センサ研究発表会
• [Presentation] Development of Semiconductor-Based Biosensor for Detection of Buckwheat Allergenic Protein in Food Products (2017)
  • Author(s): S. Hideshima, M. Saito, Y. Harada, M. Tsuna, S. Kuroiwa, T. Nakanishi, T. Osaka
  • Organizer: 232nd ECS Meeting
  • Int'l Joint Research
• [Presentation] Signal Amplification Techniques By Effective Use of the Interfacial Region for Field Effect Transistor (FET) Based Biosensing (2016)
  • Author(s): S. Hideshima, S. Wustoni, S. Kuroiwa, T. Nakanishi, T. Osaka
  • Organizer: Pacific Rim Meeting on Electrochemical and Solid-State Science 2016 (PRiME 2016)
  • Place of Presentation: Honolulu, HI
  • Year and Date: 2016-10-04
  • Int'l Joint Research