Photodissociation mechanism of heme protein studied by ultrafast spectroscopy

• Project/Area Number: 16K17784
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biological physics/Chemical physics/Soft matter physics
• Research Institution: Kanagawa University
• Principal Investigator: Yabushita Atsushi 神奈川大学, 付置研究所, 客員教授 (20376536)
• Research Collaborator: Iwakura Izumi 神奈川大学 ; Chen Pei-Feng Academia Sinica ; Lo Hsin-Pin National Chiao-Tung University
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 超高速分光 / フェムト秒レーザー / ヘム蛋白 / 光反応 / 光物性

Outline of Final Research Achievements

Heme protein plays important roles in vivo; hemoglobin transports oxygen obtained by respiration to produce energy at each part inside of the body, and nitric oxide (NO) generated by NO synthase (NOS) controls blood flow, neurotransmission, and immunity. The primary processes of these functions are triggered by ultrafast dissociation of diatomic molecules from those heme proteins. Despite the importance of the primary process, its detail mechanism is still not been elucidated.
In this project, I have developed my own ultrashort pulse laser system to study the ultrafast primary process, and applying the ultrashort pulse laser, I have elucidated the initial reaction mechanism of NOS.

Academic Significance and Societal Importance of the Research Achievements

ヘムたんぱく質は生体内で重要な機能を持っており、ヘモグロビンは呼吸で得た酸素を体内に輸送しエネルギーを与え、一酸化窒素合成酵素(NOS)から発生する一酸化窒素(NO)は血流、神経伝達、免疫をコントロールする。これらの機能のトリガーとなる初期過程は分子のヘムたんぱく質からの超高速乖離であり、本研究課題では超短パルスレーザー光源を開発することにより、NOSをはじめ様々な物質の超高速反応メカニズムを明らかにした。

Research Products

• [Int'l Joint Research] National Taiwan University/Academia Sinica, Taiwan/National Chiao-Tung University, Taiwan(その他の国・地域(台湾))
• [Int'l Joint Research] Bulgarian Academy of Sciences(ブルガリア)
• [Int'l Joint Research] Can Tho Univ. of Medicine and Pharmacy(ベトナム)
• [Int'l Joint Research] Academia Sinica/National Taiwan University/National Cheng Kung University(Taiwan)
• [Int'l Joint Research] National Taiwan University/Academia Sinica/National Chiao-Tung University(Taiwan)
• [Journal Article] Ultrafast dynamics of coherent optical phonon in a thin film of Bi3Se2Te (2018)
  • Author(s): Ying-Kuan Ko, Yan-Hao Huang, Phuoc Huu Le, Chih-Wei Luo, Marin Mirtchev Gospodinov, Takayoshi Kobayashi, Atsushi Yabushita
  • Journal Title: Chemical Physics Volume: 512 Pages: 62-67
  • DOI: 10.1016/j.chemphys.2018.03.034
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Direct observation of the change in transient molecular structure of 9,9'-bianthryl using a 10 fs pulse UV laser (2018)
  • Author(s): Sena Hashimoto, Atsushi Yabushita, Takayoshi Kobayashi, Kotaro Okamura, Izumi Iwakura
  • Journal Title: Chemical Physics Volume: 512 Pages: 128-134
  • DOI: 10.1016/j.chemphys.2017.12.016
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Experimental verification of multidimensional quantum steering (2018)
  • Author(s): Li Che-Ming、Lo Hsin-Pin、Chen Liang-Yu、Yabushita Atsushi
  • Journal Title: Optics Communications Volume: 410 Pages: 956-960
  • DOI: 10.1016/j.optcom.2017.11.070
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Transient process spectroscopy for the direct observation of inter-molecular photo-dissociation (2017)
  • Author(s): Hashimoto Sena、Yabushita Atsushi、Iwakura Izumi
  • Journal Title: Structural Dynamics Volume: 4 Issue: 5 Pages: 054901-054901
  • DOI: 10.1063/1.4983639
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Ultrafast relaxation dynamics of nitric oxide synthase studied by visible broadband transient absorption spectroscopy (2017)
  • Author(s): Hung Chih-Chang、Yabushita Atsushi、Kobayashi Takayoshi、Chen Pei-Feng、Liang Keng S.
  • Journal Title: Chemical Physics Letters Volume: 683 Pages: 619-624
  • DOI: 10.1016/j.cplett.2017.04.081
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Real-time observation of interfragment vibration and charge transfer within the TCNQF 4 dimer (2017)
  • Author(s): Hashimoto Sena、Yabushita Atsushi、Iwakura Izumi
  • Journal Title: Chemical Physics Volume: 493 Pages: 56-60
  • DOI: 10.1016/j.chemphys.2017.06.003
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Schiff Base Proton Acceptor Assists Photoisomerization of Retinal Chromophores in Bacteriorhodopsin (2017)
  • Author(s): Hung Chih-Chang、Chen Xiao-Ru、Ko Ying-Kuan、Kobayashi Takayoshi、Yang Chii-Shen、Yabushita Atsushi
  • Journal Title: Biophysical Journal Volume: 112 Issue: 12 Pages: 2503-2519
  • DOI: 10.1016/j.bpj.2017.05.015
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Ultrafast dynamics of ligand and substrate interaction in endothelial nitric oxide synthase under Soret excitation (2016)
  • Author(s): Chih-Chang Hung, Atsushi Yabushita, Takayoshi Kobayashi, Pei-Feng Chen, Keng S. Liang
  • Journal Title: Biophysical Chemistry Volume: 214 Pages: 11-16
  • DOI: 10.1016/j.bpc.2016.05.001
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Real-time measurements of ultrafast electronic dynamics in the disproportionation of [TCNQ](2-)(2) using a visible sub-10 fs pulse laser (2016)
  • Author(s): Sena Hashimoto, Atsushi Yabushita, Takayoshi Kobayashi, Izumi Iwakura
  • Journal Title: Chemical Physics Letters Volume: 650 Pages: 47-51
  • DOI: 10.1016/j.cplett.2016.02.053
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Ultrafast dynamics of uracil and thymine studied using a sub-10 fs deep ultraviolet laser (2016)
  • Author(s): Ultrafast dynamics of uracil and thymine studied using a sub-10 fs deep ultraviolet laser
  • Journal Title: Physical Chemistry Chemical Physics Volume: 18 Issue: 25 Pages: 17044-17053
  • DOI: 10.1039/c5cp07861j
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Ultrafast dynamics of oxy-hemeglobin measured by UV short pulse laser (2019)
  • Author(s): Ying Kuan Ko, Takayoshi Kobayashi, Atsushi Yabushita
  • Organizer: 10th Asian Photochemistry Conference
  • Int'l Joint Research
• [Presentation] Primary reaction in proteins observed by ultrashort pulse lasers (2019)
  • Author(s): Atsushi Yabushita
  • Organizer: Winter School on Free Electron Lasers 2019
  • Invited
• [Presentation] Ultrafast pump-probe on protein dynamics (2018)
  • Author(s): Atsushi Yabushita
  • Organizer: Introduction of Synchrotron and Laser Techniques in Biomedical Research
  • Invited
• [Presentation] Ultrafast spectroscopy of bacteriorhodopsin studied by ultrashort visible pulse laser (2018)
  • Author(s): Atsushi Yabushita, Ying-Kuan. Ko, Nai-Hong Yo, Takayoshi Kobayashi, ChihShen Yang
  • Organizer: 73rd Annual Meeting (2018), The Physical Society of Japan
• [Presentation] Primary reaction in proteins observed by ultrashort pulse lasers (2018)
  • Author(s): Atsushi Yabushita
  • Organizer: Winter School on Free Electron Laser 2018
  • Invited
• [Presentation] Ultrafast spectroscopy of nitric oxide synthase probed by broad-band visible femtosecond laser pulse (2017)
  • Author(s): Atsushi Yabushita, Chih-Chang Hung, Takayoshi Kobayashi, Pei-Feng Chen, Kent S. Liang
  • Organizer: 72nd Annual Meeting of Physical Society of Japan
  • Place of Presentation: Osaka University, Osaka, Japan
  • Year and Date: 2017-03-17
• [Presentation] Rapid-Scanning Pump-probe Spectroscopy system synchronized with 10-kHz repetition rate of Femtosecond Laser (2017)
  • Author(s): S. Kato, A. Yabushita, K. Misawa
  • Organizer: レーザー学会 第37回年次大会
  • Place of Presentation: Tokushima University, Tokushima, Japan
  • Year and Date: 2017-01-07
• [Presentation] Photo-reaction in bimolecule aggregate visualized by real-time transition state spectroscopy (2017)
  • Author(s): S. Hashimoto, A. Yabushita, T. Kobayashi, I. Iwakura
  • Organizer: Femtochemistry 13
  • Int'l Joint Research
• [Presentation] Entangle polarization of whole photon pairs generated in beam shape and in doughnuts shape (2017)
  • Author(s): Atsushi Yabushita
  • Organizer: Workshop on Quantum Science and Technology (QST)
  • Int'l Joint Research / Invited
• [Presentation] Expression of pharaonis phoborhodopsin in Eschericha coli and mesurements of fluorescence excitation and nanosecond time-resolved difference absorption spectra (2016)
  • Author(s): H. Mukawa, Y. Obara, Y. Hukutani , S. Hashimoto, I. Iwakura, A. Yabushita, M. Yohda and K. Misawa
  • Organizer: 第27回光物性研究会
  • Place of Presentation: Kobe Univeristy, Japan
  • Year and Date: 2016-12-02
• [Presentation] ジアニオン生成過程に対する電子吸引基の効果 (2016)
  • Author(s): Sena Hashimoto, Atsushi Yabushita, Izumi Iwakura
  • Organizer: 第72回有機合成化学協会関東支部シンポジウム(新潟シンポジウム)
  • Place of Presentation: Niigata University of Pharmacy and Applied Life Science, Niigata, Japan
  • Year and Date: 2016-11-26
• [Presentation] 超高速二次元相関分光法によるバクテリオロドプシンのプロトンポンプ機構解明 (2016)
  • Author(s): Atsushi Yabushita, Chii-Shen Yang, Takayoshi Kobayashi
  • Organizer: 第72回有機合成化学協会関東支部シンポジウム(新潟シンポジウム)
  • Place of Presentation: Niigata University of Pharmacy and Applied Life Science, Niigata, Japan
  • Year and Date: 2016-11-26
• [Presentation] Ultrafast dynamics of nitric oxide synthase studied by two dimensional correlation spectroscopy (2016)
  • Author(s): Atsushi Yabushita, Chih-Chang Hung, Takayoshi Kobayashi, Pei-Feng Chen, and Keng S. Liang
  • Organizer: Stereodynamics 2016
  • Place of Presentation: Academia Sinica, Taipei, Taiwan
  • Year and Date: 2016-11-06
  • Int'l Joint Research
• [Presentation] Intermolecular Disproportionation between Two Radical Anions Visualized by Real-Time Transition State Spectroscopy (2016)
  • Author(s): Sena Hashimoto, Atsushi Yabushita, Izumi Iwakura
  • Organizer: Stereodynamics 2016
  • Place of Presentation: Academia Sinica, Taipei, Taiwan
  • Year and Date: 2016-11-06
  • Int'l Joint Research
• [Presentation] Ultrafast dynamics of proton pump sub-channels in bacteriorhodopsin (2016)
  • Author(s): Atsushi Yabushita, Chih-Chang Hung, Ying-Kuan Ko, Chii-Shen Yang, Takayoshi Kobayashi
  • Organizer: Annual Meeting on Photochemistry 2016
  • Place of Presentation: The University of Tokyo, Tokyo, Japan
  • Year and Date: 2016-09-06
• [Presentation] Femtosecond time-resolved spectroscopy of bacteriorhodopsin from haloquadratum walsbyi (2016)
  • Author(s): Chih-Chang Hung, Takayoshi Kobayashi, Chii-Shen Yang, Atsushi Yabushita
  • Organizer: The 21st Biophysics Conference
  • Place of Presentation: National Tsing-Hua University, Taiwan
  • Year and Date: 2016-05-18
• [Presentation] Ultrafast dynamics of oxygenase domain of endothelial nitric oxide synthase (2016)
  • Author(s): Atsushi Yabushita, Chih-Chang Hung, Takayoshi Kobayashi, Pei-Feng Chen, Keng S. Liang
  • Organizer: The 21st Biophysics Conference
  • Place of Presentation: National Tsing-Hua University, Taiwan
  • Year and Date: 2016-05-18
• [Presentation] Ultrafast spectroscopy of oxygenase domain of endothelial nitric oxide synthase (2016)
  • Author(s): Atsushi Yabushita
  • Organizer: XXVI-th IUPAC Symposium on Photochemistry (2016 IUPAC Photochem)
  • Place of Presentation: Osaka City Central Public Hall, Osaka, Japan
  • Year and Date: 2016-04-03
  • Int'l Joint Research
• [Presentation] Two-dimensional correlation spectroscopy for observation of ultrafast dynamics of bacteriorhodopsin from haloquadratum walsybi (2016)
  • Author(s): Takayoshi Kobayashi, Chii-Shen Yang, Atsushi Yabushita
  • Organizer: XXVI-th IUPAC Symposium on Photochemistry (2016 IUPAC Photochem)
  • Place of Presentation: Osaka City Central Public Hall, Osaka, Japan
  • Year and Date: 2016-04-03
  • Int'l Joint Research
• [Presentation] Generation of polarization entangled photon pairs in shape of beam and doughnuts (2016)
  • Author(s): Atsushi Yabushita
  • Organizer: AMO (atomic, molecular, and optical physics) seminar
  • Place of Presentation: National Tsing-Hua University, Taiwan
  • Invited
• [Presentation] Femtosecond Laser Pulse for Ultrafast Spectral Snapshots of Chemical Reactions (2016)
  • Author(s): Atsushi Yabushita
  • Organizer: Institute of Global Innovation Research (GIR) Open Seminar
  • Place of Presentation: Tokyo University of Agriculture and Technology, Tokyo, Japan
  • Invited