Investigation of the Interplanetary Transfer of Microbes in the Tanpopo Mission at the Exposed Facility of the International Space Station

• Project/Area Number: 16K17840
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Geochemistry/Cosmochemistry
• Research Institution: Chiba Institute of Technology (2018); Tokyo University of Pharmacy and Life Science (2016-2017)
• Principal Investigator: KAWAGUCHI Yuko 千葉工業大学, 惑星探査研究センター, 研究員 (00733990)
• Research Collaborator: MILOJEVIC Tatyana ウィーン大学 ; OTTO Emanuel ウィーン大学 ; YAMAGISHI Akihiko 東京薬科大学, 生命科学部 ; YOKOBORI Shin-ichi 東京薬科大学, 生命科学部 ; AOKI Motohide 東京薬科大学, 生命科学部 ; YATABE June 東京薬科大学, 生命科学部 ; FUJIWARA Daisuke 東京薬科大学, 生命科学部 ; TANIGUCHI Kie 東京薬科大学, 生命科学部 ; NARUMI Issay 東洋大学, 生命科学部 ; HASHIMOTO Hirofumi JAXA ; YANO Hajime JAXA ; OKUDAIRA Kyoko 会津大学 ; NAKAGAWA Kazumichi 神戸大学 ; TABATA Makoto 千葉大学
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 微生物 / 国際宇宙ステーション / パンスペルミア / パンスペルミア仮説 / 生存 / DNA損傷 / アストロバイオロジー / 極限環境 / パンスペルミア説 / 宇宙生存 / 宇宙実験

Outline of Final Research Achievements

The “panspermia” hypothesis proposes the interplanetary transfer of life propelled by solar radiation pressure. To investigate microbial survival and DNA damage induced in space, here we placed dried cells of the radioresistant bacteria Deinococcus spp. in aluminium plate wells in exposure panels attached to the outside of the International Space Station during Tanpopo mission for 1 and 2 years. Although dried deinococcal cells of 100-um thickness died, those of 500-um thickness were alive after 2 years of space exposure. By contrast, UV rays reached only the surface of bigger cell aggregates, and the dead surface cells protected the internal dried cells. Thus, cell aggregates of 500-um thickness had sufficient protection from UV and endured the space environment.

Academic Significance and Societal Importance of the Research Achievements

微生物の曝露実験は数多く行われてきたが、これらの実験の全ては、１回だけ曝露であったため生存能力の時間依存性をみることはできなかった。本研究は、同一の場所で3年間曝露し、生存の時間依存性を解明し、長期生存率を推定できるようになる点が独創的な点であり、宇宙科学分野で重要な結果となる。

Research Products

• [Int'l Joint Research] Biophysical Chemistry/University of Vienna(オーストリア)
• [Int'l Joint Research] ウィーン大学(オーストリア)
• [Int'l Joint Research] University Wine(オーストリア)
• [Journal Article] Proteomic and Metabolomic profiling of Deinococcus radiodurans recovering after exposure to simulated Low Earth Orbit vacuum conditions (2019)
  • Author(s): Emanuel Ott, Yuko Kawaguchi, Natalie Ozgen, Akihiko Yamagishi, Elke Rabbow, Petra Rettberg, Wolfram Weckwerth, Tetyana Milojevic
  • Journal Title: Frontiers in Microbiology Volume: 10 Pages: 1-17
  • DOI: 10.3389/fmicb.2019.00909
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] STXM-XANES analyses of Murchison meteorite samples captured by aerogel after hypervelocity impacts: A potential implication of organic matter degradation for micrometeoroid collection experiments (2019)
  • Author(s): Kebukawa Yoko、Okudaira Kyoko、Yabuta Hikaru、Hasegawa Sunao、Tabata Makoto、Furukawa Yoshihiro、Ito Motoo、Nakato Aiko、Kilcoyne A. L. David、Kobayashi Kensei、Yokobori Shin-ichi、Imai Eiichi、Kawaguchi Yuko、Yano Hajime、Yamagishi Akihiko
  • Journal Title: GEOCHEMICAL JOURNAL Volume: 53 Issue: 1 Pages: 53-67
  • DOI: 10.2343/geochemj.2.0549
  • NAID: 130007593227
  • ISSN: 0016-7002, 1880-5973
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Investigation of Interplanetary Transfer of Microbes Using the International Space Station (2018)
  • Author(s): 河口 優子
  • Journal Title: Aeronautical and Space Sciences Japan Volume: 66 Issue: 9 Pages: 263-266
  • DOI: 10.14822/kjsass.66.9_263
  • NAID: 130007481404
  • ISSN: 0021-4663, 2424-1369
  • Year and Date: 2018-09-05
  • Peer Reviewed / Open Access
• [Journal Article] Tanpopo: Organics, Microbe Exposure and Micrometeorite, Microbe Capture Experiment (2018)
  • Author(s): 山岸 明彦、河口 優子、横堀 伸一、橋本 博文、矢野 創、今井 栄一、田端 誠、小林 憲正、三田 肇
  • Journal Title: Aeronautical and Space Sciences Japan Volume: 66 Issue: 6 Pages: 173-179
  • DOI: 10.14822/kjsass.66.6_173
  • NAID: 130007384401
  • ISSN: 0021-4663, 2424-1369
  • Year and Date: 2018-06-05
  • Peer Reviewed / Open Access
• [Journal Article] Environmental Data and Survival Data of Deinococcus aetherius from the Exposure Facility of the Japan Experimental Module of the International Space Station Obtained by the Tanpopo Mission (2018)
  • Author(s): A. Yamagishi, Y. Kawaguchi, H. Hashimoto, H. Yano, E. Imai, S. Kodaira, Y. Uchihori, and K. Nakagawa
  • Journal Title: Astrobiology Volume: 18 Issue: 11 Pages: 1369-1374
  • DOI: 10.1089/ast.2017.1751
  • Peer Reviewed / Open Access
• [Journal Article] Draft genome sequence of the radioresistant bacterium Deinococcus aerius TR0215, isolated from the high atmosphere above Japan (2018)
  • Author(s): Katsuya Satoh、 Hiroki Arai、Toshihiko Sanzen、Yuko Kawaguchi、Hidenori Hayashi、Shin-icni Yokobori、Akihiko Yamagishi、Yutaka Oono、Issay Narumi
  • Journal Title: Genome Announcements Volume: 6 Issue: 9 Pages: 1-1
  • DOI: 10.1128/genomea.00080-18
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 生物材料インデックス : 研究室の片隅で生き物への愛を語る 世界で最も放射線に強い細菌Deinococcus radiodurans (2018)
  • Author(s): 山岸明彦、河口優子
  • Journal Title: 生物工学会誌 Volume: 95 Pages: 295-297
  • NAID: 40021588515
• [Journal Article] 国際宇宙ステーション船外部を利用した微生物惑星間移動の検証:宇宙実験「たんぽぽ」 (2018)
  • Author(s): 河口優子
  • Journal Title: 月間「細胞」 Volume: 50 Pages: 40-42
• [Journal Article] 微生物の長期宇宙生存の科学的検証：宇宙実験「たんぽぽ」 (2018)
  • Author(s): 河口優子
  • Journal Title: 生物工学学会誌 Volume: 95 Pages: 295-297
• [Journal Article] Proteometabolomic response of Deinococcus radiodurans exposed to UVC and vacuum conditions: initial studies prior to the Tanpopo space mission (2017)
  • Author(s): Ott, E., Kawaguchi, Y., Kawagychi D., Chaturvedi, P., Nakagawa, K., Yamagishi, A., Weckwerth, W., Milojevic, T.,
  • Journal Title: PLoS One Volume: e0189381 Issue: 12 Pages: 12-12
  • DOI: 10.1371/journal.pone.0189381
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Space as a Tool for Astrobiology: review and recommendations for experimentations in Earth orbit and beyond (2017)
  • Author(s): Cottin, H., Kotler, J.M., Billi, D., Cockell, C., Demets, R., Ehrenfreund, P., Elsaesser, A., d'Hendecourt, L., van Loon, J., Martins, Z., Onofri, S., Quinn, R., Rabbow, E., Rettberg, P., Ricco, Slenzka, A. J., K., de la Torre, R., de Vera, J.-P., Westall, F., Carrasco, N., Fresneau, A., Kawaguchi, Y., et al.
  • Journal Title: Space Sci. Rev. Volume: 209 Issue: 1-4 Pages: 83-181
  • DOI: 10.1007/s11214-017-0369-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Space as a tool for astrobiology: review and recommendations for experimentations in Earth orbit and beyond. (2017)
  • Author(s): Cottin,H.,Kotler,JM.,Billi,D.,Cockell,D.,Demets,R.,Elsaesser,P.,d'Hendecourt,L.,vanLook,J.,Martins,Z.,Onofri,S.,Quimm,R.,Rabbow,E.,Rettberg,P.,Ricco,A.,Slenzka,K.,delaTorre,R.,deVera,J.,Westall,F.,Carrasco,N.,Fresseau,A.,Kawaguchi,Y.,Kebukawa,Y.,Nguyen,D.,Poch,O.,Saiagh,K., Stalport,F., Yamagishi,A., Yano,H., Klamm,BA.
  • Journal Title: Space Science Reviews Volume: 印刷中
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Survival and DNA damage of Deinococcus spp. exposed to out-side of ISS in the Tanpopo mission (2019)
  • Author(s): Kawaguchi Yuko
  • Organizer: Cosmic Labo
• [Presentation] Survival and DNA damage of cell-aggregate of Deinococcus spp. exposed to space for two-years in Tanpopo mission (2018)
  • Author(s): Kawaguchi, Y., Shibuya, M., Kinoshita, I., Hayashi, R., Yatabe, J., Narumi, I., Fujiwara, D., Hashimoto, H., Yokobori, S., Yamagishi, A.,
  • Organizer: 42nd COSPAR Scientific Assembly
  • Int'l Joint Research
• [Presentation] Survival and DNA damage of cell-aggregate of Deinococcus spp. exposed to space for two-years in Tanpopo mission (2018)
  • Author(s): Kawaguchi, Y., Shibuya, M., Kinoshita, I., Hayashi, R., Yatabe, J., Narumi, I., Fujiwara, D., Hashimoto, H., Yokobori, S., Yamagishi, A.,
  • Organizer: European Astrobiology Network Association (EANA) 2018
  • Int'l Joint Research
• [Presentation] 宇宙実験たんぽぽ計画における微生物捕集と曝露実験の科学成果 (2018)
  • Author(s): 河口優子, 澁谷美緒,木下伊織, 藤原大佑, 矢田部純, 林梨沙子,横堀伸一, 橋本博文, 鳴海一成, 山岸明彦
  • Organizer: 宇宙生物科学会第33回大会
• [Presentation] 乾燥耐性に寄与する新規カロテノイドの代謝機構の解明 (2018)
  • Author(s): 河口優子
  • Organizer: 第６回 宇宙における生命ワークショップ
• [Presentation] 微生物の宇宙生存とDNA損傷 (2018)
  • Author(s): 河口優子、澁谷美緒、林梨沙子、藤原大佑、矢田部純、谷口紀恵、青木元秀、鳴海一成、橋本博文、横堀伸一、山岸明彦
  • Organizer: 第18回宇宙科学シンポジウム
• [Presentation] 宇宙空間での微生物凝集体長期存を検証 (たんぽぽ計画) (2018)
  • Author(s): 河口優子、澁谷美緒、木下伊織、藤原大佑、矢田部純、林梨沙子、鳴海一成、橋本博文、横堀伸一、山岸明彦
  • Organizer: 生命の起原および進化学会第43回学術講演会
• [Presentation] 宇宙でDeinococcus radioduransR1に生じたDNA損傷の解析 (2018)
  • Author(s): 矢田部純、河口優子、木下伊織、藤原大祐、青木元秀、谷口紀恵、鳴海一成、澁谷美緒、橋本博文、横堀伸一、山岸明彦
  • Organizer: 生命の起原および進化学会第43回学術講演会
• [Presentation] 宇宙環境下で生じる Deinococcus radiodurans R1のrpoB遺伝子の突然変異 (2018)
  • Author(s): 藤原大佑、河口優子、冨樫油香、木下伊織、矢田部純、鳴海一成、橋本博文、横堀伸一、山岸明彦
  • Organizer: 生命の起原および進化学会第43回学術講演会
• [Presentation] 長期乾燥耐性に関与するカロテノイドの探索 (2018)
  • Author(s): 林梨沙子、河口優子、青木元秀、野呂幸佑、橋本博文、横堀伸一、山岸明彦
  • Organizer: 生命の起原および進化学会第43回学術講演会
• [Presentation] 宇宙実験「たんぽぽ計画」を例にした研究との付き合い方 (2018)
  • Author(s): 河口優子
  • Organizer: 次世代科学社会応援公開シンポジウム2018日本の科学と文化；世界そして宇宙へ
  • Invited
• [Presentation] 500 μm cell-aggregation of Deinococcus spp. was enough thickness to survive after 384 days exposure at ISS orbit in Tanpopo mission. (2017)
  • Author(s): Y., Kawaguchi, I., Kinoshita, J., Yatabe, Y., Murano, D., Fujiwara, M., Harada, K., Nakagawa, I., Narumi, S., Kodaira, Y., Uchihori, H., Hashimoto, S., Yokobori, A., Yamagish,
  • Organizer: JpGU-AGU Joint Meeting 2017
  • Place of Presentation: 幕張メッセ、幕張、千葉
  • Year and Date: 2017-05-20
  • Int'l Joint Research
• [Presentation] 「生命が宇宙を旅する?! 宇宙実験 (たんぽぽ計画)」 (2017)
  • Author(s): 河口優子
  • Organizer: 第2回Keio Astrobiology Camp 2017
  • Place of Presentation: 慶応大学鶴岡キャンパス、山形
  • Year and Date: 2017-03-26
  • Invited
• [Presentation] Analysis of survival and DNA damage of space exposed Deinococcus spp. (2017)
  • Author(s): 河口, 優子; 木下, 伊織; 藤原, 大佑; 矢田部, 純; 村野, 由佳; 青木, 元秀; 谷口, 紀恵; 鳴海, 一成; 林, 宣宏; 中川, 和道; 橋本, 博文; 横堀, 伸一; 山岸, 明彦
  • Organizer: 第17回宇宙科学シンポジウム 講演集
  • Place of Presentation: 宇宙航空研究開発機構宇宙科学研究所(JAXA)(ISAS)相模原キャンパス), 相模原市, 神奈川県
  • Year and Date: 2017-01-05
• [Presentation] 500μm cell-aggregation of Deinococcus spp. was enough thickness to survive after 384 days exposure at ISS orbit in Tanpopo mission (2017)
  • Author(s): Kawaguchi, Y., Kinoshita, I., Yatabe, J., Murano, Y., Fujiwara, D., Harada, M., Nakagawa, K., Narumi, I., Kodaira, S., Uchihori, Y., Hashimoto, H., Yokobori, S., Yamagishi, A
  • Organizer: JpGU-AGU Joint Meeting 2017
  • Int'l Joint Research
• [Presentation] A., 500 μm cell-aggregate of Deinoccoccus spp. was sufficient to survive after one-year exposure on ISS orbit in Tanpopo mission (2017)
  • Author(s): Kawaguchi, Y., Kinoshita, I. Yatabe, J. Murano, Y. Fujiwara, D. Hayashi, R. Harada, M. Nakagawa, K. Narumi, I., Kodaira, S., Uchihori, Y., Hashimoto, H., Yokobori, S., Yamagishi
  • Organizer: EANA2017
  • Int'l Joint Research
• [Presentation] 国際宇宙ステーション曝露部での微生物宇宙生存機構解析と採集実験 (2017)
  • Author(s): 河口優子
  • Organizer: 環境微生物系学会合同大会2017
  • Invited
• [Presentation] 500 μm厚のDeinococcus属の細胞凝集体は1年間宇宙で生存できる (2017)
  • Author(s): 河口優子、木下伊織、矢田部純、谷口紀恵、鳴海一成、林梨沙子、藤原大佑、村野由佳、青木元秀、橋本博文、横堀伸一、山岸明彦
  • Organizer: 日本宇宙生物科学会第31回大会
• [Presentation] 微生物の惑星間移動の検証：たんぽぽ計画 (2017)
  • Author(s): 河口優子
  • Organizer: 生命科学系学会合同年次大会（ConBio2017）
  • Invited
• [Presentation] Analysis of DNA damage in the radiation resistant microbe Deinococcus radiodurans R1 exposed to space in Tanpopo mission (2017)
  • Author(s): Yatabe, J., Kawaguchi, Y., Kinoshita, I., Fujiwara, D., Aoki, M., Taniguchi, K., Narumi, I., Iwai, S., Yamamoto, J., Hashimoto, H., Yokobori, S., Yamagishi, A.
  • Organizer: JpGU-AGU Joint Meeting 2017
  • Int'l Joint Research
• [Presentation] Mutation analysis of the rpoB gene in the radiation-resistant bacterium Deinococcus radiodurans R1 exposed to space (2017)
  • Author(s): Fujiwara, D., Kawaguchi, Y., Togashi, Y., Iori, K., Yatabe, J., Narumi, I., Nakagawa, K., Hashimoto, H., Yokobori, S., Yamagishi. A.
  • Organizer: JpGU-AGU Joint Meeting 2017
  • Int'l Joint Research
• [Presentation] 生命が宇宙を旅する (2017)
  • Author(s): 河口優子
  • Organizer: Keio Astrobiology Camp2017
• [Presentation] たんぽぽ初年度試料の分析結果速報 (2017)
  • Author(s): 河口優子
  • Organizer: アストロバイオロジー特別講演会「深海と深宇宙の生命探査」
  • Invited
• [Presentation] 宇宙でDeinococcus radiodurans R1に生じたDNA損傷 (2017)
  • Author(s): 矢田部純、河口優子、木下伊織、藤原大佑、青木元秀、谷口紀恵、鳴海一成、澁谷美穂、橋本博文、横堀伸一、山岸明彦
  • Organizer: 日本宇宙生物科学会第31回大会
• [Presentation] 宇宙環境下で生じるDeinococcus radiodurans R1のrpoB遺伝子の突然変異 (2017)
  • Author(s): 藤原大佑、河口優子、富樫油香、木下伊織、矢田部純、鳴海一成、中川和道、橋本博文、横堀伸一、山岸明彦
  • Organizer: 日本宇宙生物科学会第31回大会
• [Presentation] たんぽぽ計画微生物実験 (2017)
  • Author(s): 河口優子
  • Organizer: サイエンスアゴラ2017宇宙での生命と有機物探査：たんぽぽ計画とアストロバイオロジーの今後の展開
  • Invited
• [Presentation] The ground-based experiments of survival test and DNA-damage analyses of Deinococcus spp. after exposure to simulated space conditions for Tanpopo mission. (2016)
  • Author(s): Y., Kawaguchi, D., Fujiwara, I., Kinoshita, J., Yatabe, I., Narumi, M., Aoki, S., Yokobori, H., Hashimoto, K., Nakagawa and A., Yamagishi,
  • Organizer: 16th European Workshop on Astrobiology
  • Place of Presentation: アテネ、ギリシャ
  • Year and Date: 2016-09-27
  • Int'l Joint Research
• [Presentation] 模擬宇宙環境で生じたDeinococcus属細菌のDNA損傷の解明 (2016)
  • Author(s): 河口優子、木下伊織、藤原大佑、矢田部純、鳴海一成、青木元秀、中川和道、橋本博文、横堀伸一、山岸明彦
  • Organizer: アストロバイオロジーネットワーク2016年年会
  • Place of Presentation: 東北大学、仙台、宮城
  • Year and Date: 2016-09-22
• [Presentation] 国際宇宙ステーション曝露部を利用した惑星間での微生物の移動検証 (2016)
  • Author(s): 河口 優子, 横堀 伸一, 橋本 博文, 矢野 創, 富田-横谷 香織, 林 宣宏, 鳴海 一成, 今井 栄一, 田端 誠, 河合 秀幸, 奥平 恭子, 佐々木 聰, 中川 和道, 山岸 明彦
  • Organizer: 第60回宇宙科学技術連合講演会
  • Place of Presentation: 函館、北海道
  • Year and Date: 2016-09-06
• [Presentation] 「生命が宇宙を旅する?! 宇宙実験 (たんぽぽ計画)」 (2016)
  • Author(s): 河口優子
  • Organizer: 出張someoneセミナー うわさのハカセに会おう！、株式会社リバネス主催
  • Place of Presentation: TEPIA先端技術館、東京
  • Invited
• [Book] Panspermia Hypothesis: History of a Hypothesis and a Review of the Past, Present, and Future Planned Missions to Test This Hypothesis (2019)
  • Author(s): Yuko Kawaguchi
  • Total Pages: 10
  • Publisher: Springer, Singapore
• [Remarks] たんぽぽ計画
  • URL: http://www.ls.toyaku.ac.jp/~lcb-7/tanpopo/
• [Remarks] 極限環境生物学研究室 河口優子
  • URL: http://www.ls.toyaku.ac.jp/~lcb-7/kawaguchi/index.html