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Observation of three-dimensional structural dynamics in biological chemical reactions based on Raman optical activity

Research Project

Project/Area Number 16K17859
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Physical chemistry
Research InstitutionSaga University

Principal Investigator

Fujisawa Tomotsumi  佐賀大学, 工学(系)研究科(研究院), 特任講師 (60633493)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywordsラマン光学活性 / 光受容タンパク質 / 反応中間体 / 反応中間体測定 / 生体分子科学 / 振動分光学
Outline of Final Research Achievements

This research have been aiming to develop Raman optical acitivity (ROA) spectroscopy to track the three-dimensional (3D) structure of the reaction intermediates in photoreceptor proteins. We successfully measured the ROA spectrum for the transient state of orange cartenoid protein and analysed the 3D structure. We also built the low-temperature Raman setup to measure the photo-itnermediates of various photoreceptors . We obtained good Raman data for the photo-intermediates of a couple of photoreceptors, which allows us to apply ROA spectroscopy to the photointermediates.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (24 results)

All 2018 2017 2016 Other

All Int'l Joint Research (2 results) Journal Article (9 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 9 results,  Open Access: 1 results,  Acknowledgement Compliant: 3 results) Presentation (10 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Remarks (3 results)

  • [Int'l Joint Research] ミシガン州立大学(米国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] ミシガン州立大学(米国)

    • Related Report
      2016 Research-status Report
  • [Journal Article] Light-induced chromophore and protein responses and mechanical signal transduction of BLUF proteins2017

    • Author(s)
      Tomotsumi Fujisawa, Shinji Masuda
    • Journal Title

      Biophysical Reviews

      Volume: 10 Issue: 2 Pages: 327-337

    • DOI

      10.1007/s12551-017-0355-6

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of an Azo-Based Photosensitizer Activated under Mild Hypoxia for Photodynamic Therapy2017

    • Author(s)
      Piao Wen、Hanaoka Kenjiro、Fujisawa Tomotsumi、Takeuchi Satoshi、Komatsu Toru、Ueno Tasuku、Terai Takuya、Tahara Tahei、Nagano Tetsuo、Urano Yasuteru
    • Journal Title

      J. Am. Chem. Soc.

      Volume: 139 Issue: 39 Pages: 13713-13719

    • DOI

      10.1021/jacs.7b05019

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Raman Optical Activity Reveals Carotenoid Photoactivation Events in the Orange Carotenoid Protein in Solution2017

    • Author(s)
      Fujisawa Tomotsumi、Leverenz Ryan L.、Nagamine Momoka、Kerfeld Cheryl A.、Unno Masashi
    • Journal Title

      J. Am. Chem. Soc.

      Volume: 139 Issue: 30 Pages: 10456-10460

    • DOI

      10.1021/jacs.7b05193

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Transient Resonance Raman Spectroscopy of a Light-Driven Sodium-Ion-Pump Rhodopsin from Indibacter alkaliphilus2017

    • Author(s)
      Kajimoto Kousuke、Kikukawa Takashi、Nakashima Hiroki、Yamaryo Haruki、Saito Yuta、Fujisawa Tomotsumi、Demura Makoto、Unno Masashi
    • Journal Title

      J. Phys. Chem. B

      Volume: 121 Issue: 17 Pages: 4431-4437

    • DOI

      10.1021/acs.jpcb.7b02421

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Broadband Stimulated Raman Spectroscopy in the Deep Ultraviolet Region2017

    • Author(s)
      H. Kuramochi, T. Fujisawa, S. Takeuchi, T. Tahara
    • Journal Title

      Chemical Physics Letters

      Volume: 印刷中 Pages: 543-546

    • DOI

      10.1016/j.cplett.2017.02.015

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Development of an Azoreductase-based Reporter System with Synthetic Fluorogenic Substrates2017

    • Author(s)
      Narae Shin, Kenjiro Hanaoka, Wen Piao, Takuya Miyakawa, Tomotsumi Fujisawa, Satoshi Takeuchi, Shodai Takahashi, Toru Komatsu, Tasuku Ueno, Takuya Terai, Tahei Tahara, Masaru Tanokura, Tetsuo Nagano, Yasuteru Urano
    • Journal Title

      ACS Chem. Biol.

      Volume: 12 Issue: 2 Pages: 558-563

    • DOI

      10.1021/acschembio.6b00852

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Raman optical activity of tetra-alanine in the poly(L-proline) II type peptide conformation2017

    • Author(s)
      Masakazu Furuta, Tomotsumi Fujisawa, Hiroyasu Urago, Takahiro Eguchi, Takahito Shingae, Satoshi Takahashi, Ewan W. Blanch, Masashi Unno
    • Journal Title

      Phys. Chem. Chem. Phys.

      Volume: 19 Issue: 3 Pages: 2078-2086

    • DOI

      10.1039/c6cp07828a

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] ラマン光学活性分光法2016

    • Author(s)
      藤澤知績、 海野雅司
    • Journal Title

      分光研究

      Volume: 65 Pages: 218-231

    • NAID

      40020995115

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Ultrafast Dynamics in Aromatic Cation Based Ionic Liquids: A Femtosecond Raman-Induced Kerr Effect Spectroscopic Study2016

    • Author(s)
      Hideaki Shirota, Shohei Kakinuma, Kotaro Takahashi, Akito Tago, Hocheon Jeong, Tomotsumi Fujisawa
    • Journal Title

      Bulletin of the Chemical Society of Japan

      Volume: 89 Issue: 9 Pages: 1106-1128

    • DOI

      10.1246/bcsj.20160085

    • NAID

      130006770695

    • ISSN
      0009-2673, 1348-0634
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Anharmonically-coupled low frequency mode in green fluorescent protein: the assignment by QM/MM calculation2018

    • Author(s)
      Tomotsumi Fujisawa, Masashi Unno
    • Organizer
      日本化学会 第98春季年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Searching for Anharmonic Low Frequency Mode in Green Fluorescent Protein2017

    • Author(s)
      藤澤知績、海野雅司
    • Organizer
      日本化学会 第97春季年会
    • Place of Presentation
      慶応義塾大学 日吉キャンパス
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] Time-resolved impulsive stimulated Raman study of green fluorescent protein: role of a coherent low frequency mode in the excited state proton transfer2017

    • Author(s)
      藤澤知績
    • Organizer
      第77回岡崎カンファレンス
    • Place of Presentation
      分子科学研究所
    • Year and Date
      2017-03-06
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Evidence for colse-to-open photoactivation of orange carotenoid protein from ultraviolet resonance Raman spectroscopy2017

    • Author(s)
      Yushi Nakamizo, Momoka Nagamine, Tomotsumi Fujisawa, Cheryl Kerfeld, Masashi Unno
    • Organizer
      日本生物物理学会 第55回年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ラマン光学活性分光を用いた微生物型ロドプシンにおけるレチナール発色団のコンフォメーション解析2017

    • Author(s)
      松尾淳平、菊川峰志、海野雅司、藤澤知績
    • Organizer
      日本生物物理学会 第55回年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Functional importance of trimer formation of light-driven H+ pump Gloeobacter rhodopsin2017

    • Author(s)
      Azusa Iizuka, Takashi Kikukawa, Kosuke Kajimoto, Tomotsumi Fujisawa, Takashi Tsukamoto, Tomoyasu Aizawa, Naoki Kamo, Masashi Unno, Makoto Demura
    • Organizer
      日本生物物理学会 第55回年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ナトリウムポンプ型ロドプシン中間体の過渡共鳴ラマン分光法による研究2017

    • Author(s)
      梶本航介、菊川峰志、海野雅司、藤澤知績
    • Organizer
      日本生物物理学会 第55回年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Chromophore conformation in active site of orange carotenoid protein studied by Raman optical activity spectroscopy2016

    • Author(s)
      藤澤知績, 海野雅司,Ryan L. Leverenz, Cheryl A. Kerfeld
    • Organizer
      第54回日本生物物理学会年会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2016-11-25
    • Related Report
      2016 Research-status Report
  • [Presentation] Chromophore conformation in active site of orange carotenoid protein from Raman optical activity2016

    • Author(s)
      藤澤知績, 海野雅司,Ryan L. Leverenz, Cheryl A. Kerfeld
    • Organizer
      5th International Conference on Vibrational Optical Activity
    • Place of Presentation
      アントワープ大学、ベルギー
    • Year and Date
      2016-09-11
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] ラマン光学活性に基づくオレジカロテノイドタパク質の活性部位における発色団立体構造変化 の活性部位における発色団立体構造変化 の活性部位における発色団立体構造変化 の活性部位における発色団立体構造変化 の活性部位における発色団立体構造変化 の活性部位における発色団立体構造変化2016

    • Author(s)
      藤澤知績, 海野雅司,Ryan L. Leverenz, Cheryl A. Kerfeld
    • Organizer
      第43回生物分子科学討論会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2016-06-24
    • Related Report
      2016 Research-status Report
  • [Remarks]

    • URL

      http://biophysics.chem.saga-u.ac.jp/Fujisawa_webpage/Tfujisawa.html

    • Related Report
      2017 Annual Research Report
  • [Remarks]

    • URL

      http://biophysics.chem.saga-u.ac.jp/unno-lab/homu.html

    • Related Report
      2017 Annual Research Report
  • [Remarks] 佐賀大学 海野-藤澤研究室 藤澤知績

    • URL

      http://biophysics.chem.saga-u.ac.jp/Fujisawa_webpage/Tfujisawa.html

    • Related Report
      2016 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2022-06-07  

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