Observation of three-dimensional structural dynamics in biological chemical reactions based on Raman optical activity
| Project/Area Number |
16K17859
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Physical chemistry
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| Research Institution | Saga University |
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Principal Investigator |
Fujisawa Tomotsumi 佐賀大学, 工学(系)研究科(研究院), 特任講師 (60633493)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | ラマン光学活性 / 光受容タンパク質 / 反応中間体 / 反応中間体測定 / 生体分子科学 / 振動分光学 |
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| Outline of Final Research Achievements |
This research have been aiming to develop Raman optical acitivity (ROA) spectroscopy to track the three-dimensional (3D) structure of the reaction intermediates in photoreceptor proteins. We successfully measured the ROA spectrum for the transient state of orange cartenoid protein and analysed the 3D structure. We also built the low-temperature Raman setup to measure the photo-itnermediates of various photoreceptors . We obtained good Raman data for the photo-intermediates of a couple of photoreceptors, which allows us to apply ROA spectroscopy to the photointermediates.
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Report
(3 results)
Research Products
(24 results)
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[Journal Article] Development of an Azoreductase-based Reporter System with Synthetic Fluorogenic Substrates2017
Author(s)
Narae Shin, Kenjiro Hanaoka, Wen Piao, Takuya Miyakawa, Tomotsumi Fujisawa, Satoshi Takeuchi, Shodai Takahashi, Toru Komatsu, Tasuku Ueno, Takuya Terai, Tahei Tahara, Masaru Tanokura, Tetsuo Nagano, Yasuteru Urano
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Journal Title
ACS Chem. Biol.
Volume: 12
Issue: 2
Pages: 558-563
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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