Observation of three-dimensional structural dynamics in biological chemical reactions based on Raman optical activity

• Project/Area Number: 16K17859
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Physical chemistry
• Research Institution: Saga University
• Principal Investigator: Fujisawa Tomotsumi 佐賀大学, 工学(系)研究科(研究院), 特任講師 (60633493)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: ラマン光学活性 / 光受容タンパク質 / 反応中間体 / 反応中間体測定 / 生体分子科学 / 振動分光学

Outline of Final Research Achievements

This　research　have been aiming to develop Raman optical acitivity (ROA) spectroscopy to track　the　three-dimensional (3D)　structure　of　the　reaction　intermediates in photoreceptor proteins. We successfully measured the ROA spectrum for the transient state of orange cartenoid protein and analysed the 3D structure. We also built the low-temperature Raman setup to measure the photo-itnermediates of various photoreceptors . We obtained good Raman data for the photo-intermediates of a couple of photoreceptors, which allows us to apply ROA spectroscopy to the photointermediates.

Research Products

• [Int'l Joint Research] ミシガン州立大学(米国)
• [Int'l Joint Research] ミシガン州立大学(米国)
• [Journal Article] Light-induced chromophore and protein responses and mechanical signal transduction of BLUF proteins (2017)
  • Author(s): Tomotsumi Fujisawa, Shinji Masuda
  • Journal Title: Biophysical Reviews Volume: 10 Issue: 2 Pages: 327-337
  • DOI: 10.1007/s12551-017-0355-6
  • Peer Reviewed / Open Access
• [Journal Article] Development of an Azo-Based Photosensitizer Activated under Mild Hypoxia for Photodynamic Therapy (2017)
  • Author(s): Piao Wen、Hanaoka Kenjiro、Fujisawa Tomotsumi、Takeuchi Satoshi、Komatsu Toru、Ueno Tasuku、Terai Takuya、Tahara Tahei、Nagano Tetsuo、Urano Yasuteru
  • Journal Title: J. Am. Chem. Soc. Volume: 139 Issue: 39 Pages: 13713-13719
  • DOI: 10.1021/jacs.7b05019
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Raman Optical Activity Reveals Carotenoid Photoactivation Events in the Orange Carotenoid Protein in Solution (2017)
  • Author(s): Fujisawa Tomotsumi、Leverenz Ryan L.、Nagamine Momoka、Kerfeld Cheryl A.、Unno Masashi
  • Journal Title: J. Am. Chem. Soc. Volume: 139 Issue: 30 Pages: 10456-10460
  • DOI: 10.1021/jacs.7b05193
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Transient Resonance Raman Spectroscopy of a Light-Driven Sodium-Ion-Pump Rhodopsin from Indibacter alkaliphilus (2017)
  • Author(s): Kajimoto Kousuke、Kikukawa Takashi、Nakashima Hiroki、Yamaryo Haruki、Saito Yuta、Fujisawa Tomotsumi、Demura Makoto、Unno Masashi
  • Journal Title: J. Phys. Chem. B Volume: 121 Issue: 17 Pages: 4431-4437
  • DOI: 10.1021/acs.jpcb.7b02421
  • Peer Reviewed
• [Journal Article] Broadband Stimulated Raman Spectroscopy in the Deep Ultraviolet Region (2017)
  • Author(s): H. Kuramochi, T. Fujisawa, S. Takeuchi, T. Tahara
  • Journal Title: Chemical Physics Letters Volume: 印刷中 Pages: 543-546
  • DOI: 10.1016/j.cplett.2017.02.015
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Development of an Azoreductase-based Reporter System with Synthetic Fluorogenic Substrates (2017)
  • Author(s): Narae Shin, Kenjiro Hanaoka, Wen Piao, Takuya Miyakawa, Tomotsumi Fujisawa, Satoshi Takeuchi, Shodai Takahashi, Toru Komatsu, Tasuku Ueno, Takuya Terai, Tahei Tahara, Masaru Tanokura, Tetsuo Nagano, Yasuteru Urano
  • Journal Title: ACS Chem. Biol. Volume: 12 Issue: 2 Pages: 558-563
  • DOI: 10.1021/acschembio.6b00852
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Raman optical activity of tetra-alanine in the poly(L-proline) II type peptide conformation (2017)
  • Author(s): Masakazu Furuta, Tomotsumi Fujisawa, Hiroyasu Urago, Takahiro Eguchi, Takahito Shingae, Satoshi Takahashi, Ewan W. Blanch, Masashi Unno
  • Journal Title: Phys. Chem. Chem. Phys. Volume: 19 Issue: 3 Pages: 2078-2086
  • DOI: 10.1039/c6cp07828a
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] ラマン光学活性分光法 (2016)
  • Author(s): 藤澤知績、 海野雅司
  • Journal Title: 分光研究 Volume: 65 Pages: 218-231
  • NAID: 40020995115
  • Peer Reviewed
• [Journal Article] Ultrafast Dynamics in Aromatic Cation Based Ionic Liquids: A Femtosecond Raman-Induced Kerr Effect Spectroscopic Study (2016)
  • Author(s): Hideaki Shirota, Shohei Kakinuma, Kotaro Takahashi, Akito Tago, Hocheon Jeong, Tomotsumi Fujisawa
  • Journal Title: Bulletin of the Chemical Society of Japan Volume: 89 Issue: 9 Pages: 1106-1128
  • DOI: 10.1246/bcsj.20160085
  • NAID: 130006770695
  • ISSN: 0009-2673, 1348-0634
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Anharmonically-coupled low frequency mode in green fluorescent protein: the assignment by QM/MM calculation (2018)
  • Author(s): Tomotsumi Fujisawa, Masashi Unno
  • Organizer: 日本化学会 第98春季年会
• [Presentation] Searching for Anharmonic Low Frequency Mode in Green Fluorescent Protein (2017)
  • Author(s): 藤澤知績、海野雅司
  • Organizer: 日本化学会 第97春季年会
  • Place of Presentation: 慶応義塾大学 日吉キャンパス
  • Year and Date: 2017-03-16
• [Presentation] Time-resolved impulsive stimulated Raman study of green fluorescent protein: role of a coherent low frequency mode in the excited state proton transfer (2017)
  • Author(s): 藤澤知績
  • Organizer: 第77回岡崎カンファレンス
  • Place of Presentation: 分子科学研究所
  • Year and Date: 2017-03-06
  • Int'l Joint Research / Invited
• [Presentation] Evidence for colse-to-open photoactivation of orange carotenoid protein from ultraviolet resonance Raman spectroscopy (2017)
  • Author(s): Yushi Nakamizo, Momoka Nagamine, Tomotsumi Fujisawa, Cheryl Kerfeld, Masashi Unno
  • Organizer: 日本生物物理学会 第55回年会
• [Presentation] ラマン光学活性分光を用いた微生物型ロドプシンにおけるレチナール発色団のコンフォメーション解析 (2017)
  • Author(s): 松尾淳平、菊川峰志、海野雅司、藤澤知績
  • Organizer: 日本生物物理学会 第55回年会
• [Presentation] Functional importance of trimer formation of light-driven H+ pump Gloeobacter rhodopsin (2017)
  • Author(s): Azusa Iizuka, Takashi Kikukawa, Kosuke Kajimoto, Tomotsumi Fujisawa, Takashi Tsukamoto, Tomoyasu Aizawa, Naoki Kamo, Masashi Unno, Makoto Demura
  • Organizer: 日本生物物理学会 第55回年会
• [Presentation] ナトリウムポンプ型ロドプシン中間体の過渡共鳴ラマン分光法による研究 (2017)
  • Author(s): 梶本航介、菊川峰志、海野雅司、藤澤知績
  • Organizer: 日本生物物理学会 第55回年会
• [Presentation] Chromophore conformation in active site of orange carotenoid protein studied by Raman optical activity spectroscopy (2016)
  • Author(s): 藤澤知績, 海野雅司，Ryan L. Leverenz, Cheryl A. Kerfeld
  • Organizer: 第54回日本生物物理学会年会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2016-11-25
• [Presentation] Chromophore conformation in active site of orange carotenoid protein from Raman optical activity (2016)
  • Author(s): 藤澤知績, 海野雅司，Ryan L. Leverenz, Cheryl A. Kerfeld
  • Organizer: 5th International Conference on Vibrational Optical Activity
  • Place of Presentation: アントワープ大学、ベルギー
  • Year and Date: 2016-09-11
  • Int'l Joint Research
• [Presentation] ラマン光学活性に基づくオレジカロテノイドタパク質の活性部位における発色団立体構造変化 の活性部位における発色団立体構造変化 の活性部位における発色団立体構造変化 の活性部位における発色団立体構造変化 の活性部位における発色団立体構造変化 の活性部位における発色団立体構造変化 (2016)
  • Author(s): 藤澤知績, 海野雅司，Ryan L. Leverenz, Cheryl A. Kerfeld
  • Organizer: 第43回生物分子科学討論会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2016-06-24
• [Remarks]
  • URL: http://biophysics.chem.saga-u.ac.jp/Fujisawa_webpage/Tfujisawa.html
• [Remarks]
  • URL: http://biophysics.chem.saga-u.ac.jp/unno-lab/homu.html
• [Remarks] 佐賀大学 海野-藤澤研究室 藤澤知績
  • URL: http://biophysics.chem.saga-u.ac.jp/Fujisawa_webpage/Tfujisawa.html