Creation and Characterization of functional heme-DNA
| Project/Area Number |
16K17929
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bio-related chemistry
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | DNA / ヘム / ペルオキシダーゼ活性 / G-カルテット / 四重鎖DNA / ヘム核酸 / Gカルテット |
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| Outline of Final Research Achievements |
A single DNA repeat sequence of the human telomere, d(TTAGGG), forms all parallel G-quadruplex DNA i.e., (d(TTAGGG))4. We found that heme binds to 3’-terminal G6 G-quartet of (d(TTAGGG))4 to form a stable complex. In this study, we have characterized the molecular structures of heme-(d(TTAGGGX))4 complexes, where X is vacant, A, or T, and the peroxidase activities of the complexes in order to delineate the relationship between the heme environment and peroxidase activity of the complex.
NMR analysis indicated that heme stacks onto G6-quartet of the DNA in the complex. Consequently, the interaction between heme and G6-quartet in the complex was found to be independent of the addition of the extra base at the 3’-terminal, i.e., A7 or T7.
Peroxidase activity of the heme-(d(TTAGGGA))4 complex was found to be remarkably higher than those of the other two complexes, suggesting that the 3’-terminal A of the DNA acts as a general base to enhance the catalytic activity of the complex.
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Report
(3 results)
Research Products
(20 results)
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[Presentation] ヒトテロメア類似塩基配列の四重鎖DNAとヘム複合体のペルオキシダーゼ活性と構造の関係の解析2018
Author(s)
荒木はるか, 中山優作, 越智健太郎, 篠宮僚介, 柴田友和, 逸見光, 萩原正規, 小倉尚志, 柳澤幸子, 鈴木秋弘, 根矢三郎, Sen Dipankar, 山本泰彦
Organizer
日本化学会 第98春季年会 (2018)
Related Report
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[Presentation] Heme-DNA Complexes2017
Author(s)
Y. Yamamoto, T. Shibata, Y. Katahira, K. Ochi, Y. Nakayama, H. Tai, A. Watanabe, T. Nakao, S. Yanagisawa, T. Ogura, H. Hemmi, M. Hagihara, A. Suzuki, and S. Neya
Organizer
14th International Symposium on Applied Bioinorganic Chemistry(ISABC14)
Related Report
Int'l Joint Research
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[Presentation] Characterization of Complexes between Hemes and Parallel G-Quadruplex DNAs2016
Author(s)
Y. Yamamoto, T. Shibata, Y. Katahira, Y. Nakayama, H. Tai, T. Matsui, K. Morihashi, A. Watanabe, T. Nakao, S. Yanagisawa, T. Ogura, A. Suzuki, S. Neya
Organizer
8th Asian Bioligical Inorganic Chemistry Conference(AsBIC 8)
Place of Presentation
New Zealand・Owen G Glenn Building 12 Grafton Rd, The University of Auckland
Year and Date
2016-12-04
Related Report
Int'l Joint Research
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