| Project/Area Number |
16K17930
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bio-related chemistry
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| Research Institution | Osaka University (2017)
The University of Tokyo (2016) |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | アフィニティーラベル化 / 標的タンパク質同定 / 発蛍光プローブ分子 / 創薬化学 / 生物活性化合物 / 標的タンパク質 / 標的同定 / アフィニティラベル化 / ケミカルバイオロジー / タンパク質化学修飾 |
|---|
| Outline of Final Research Achievements |
To elucidate the mechanism of bioactive small molecules, target protein identification methods are highly important. In this project, a novel affinity labeling probe having azidophthalimide structure was designed. By several model experiments, we revealed that the designed probe is suitable for selective fluorescent labeling of the target protein. Furthermore, the ligand-binding site was successfully determined by this method. We also successfully designed and synthesized several bioactive small molecules.
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