| Project/Area Number |
16K17936
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bio-related chemistry
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
Tai Hulin 奈良先端科学技術大学院大学, 物質創成科学研究科, 特任助教 (40512554)
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| Research Collaborator |
HIROTA Shun 奈良先端科学技術大学院大学, 物質創成科学研究科, 教授
HIGUCHI Yoshiki 兵庫県立大学, 生命理学研究科, 教授 (90183574)
XU Liyang
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 金属蛋白質 / Ni酵素 / ヒドロゲナーゼ / 酸塩基平衡 / 光活性化 / 水素 / FT-IR / 生体関連化学 / タンパク質・酵素化学 |
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| Outline of Final Research Achievements |
[NiFe] hydrogenase catalyzes the interconversion of dihydrogen to two protons and two electrons. The acid-base equilibrium between the ready Ni-SIr and active Ni-SIa states is a common feature among [NiFe] hydrogenases, but its mechanism remains unrevealed. We have shown that the Ni-SIr state was photo-activated to its Ni-SIa state by Ar+ laser irradiation at 514.5 nm, whereas the Ni-SL state was light induced from a newly identified state, which was less active than any other identified state and existed in the “as-isolated” enzyme. Large activation energy and kinetic isotope effect were obtained for the reconversion of the Ni-SIa state to Ni-SIr state after the Ni-SIr-to-Ni-SIa photoactivation, suggesting that the Ni-SIa state reacts with H2O and leaves a bridging hydroxo ligand for the Ni-SIr state. These results provide new insights into the acid-base equilibrium of [NiFe] hydrogenase.
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