Development and application of novel chemical modification method for analysis of unusual G-quadruplex structures
| Project/Area Number |
16K17938
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bio-related chemistry
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| Research Institution | University of Miyazaki |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 四重鎖構造 / 核酸 / ヒトテロメアRNA / 化学修飾 / 19F-NMR / フッ素 / グアニン / ヒトテロメアDNA |
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| Outline of Final Research Achievements |
In this study, we developed a novel chemical modification method to analyze unusual G-quadruplex structures in living cells. Specifically, in-cell 19F NMR spectroscopy was employed to investigate the structural features of human telomere RNA in Xenopus laevis oocytes. Only one signal was observed in the in-cell 19F NMR spectrum, for which the chemical shift is identical to that observed for the corresponding higher-order G-quadruplex in the in vitro 19F NMR spectrum. We further showed that 19F-NMR can detect DNA/RNA hybrid G-quadruplex structure. We performed a temperature dependent experiment to investigate the stability of the DNA/RNA hybrid G-quadruplex. The result indicated that 19F-NMR can be used to directly observe the melting process of the DNA/RNA hybrid G-quadruplex, which was not easily accessed by traditional methods, such as CD or UV spectroscopy.
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Report
(3 results)
Research Products
(24 results)
