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Development of a particle-manipulation method for automating the cross-match test using two-phase flow and dielectrophoresis

Research Project

Project/Area Number 16K18033
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Thermal engineering
Research InstitutionTokyo University of Science

Principal Investigator

Yamamoto Ken  東京理科大学, 工学部機械工学科, 助教 (70749100)

Research Collaborator MOTOSUKE Masahiro  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords血液 / 混合 / 希釈 / 混相流 / マイクロチャネル / 界面 / 赤血球 / 反応 / マイクロ混相流 / 混合促進 / コロイド溶液 / 気泡 / 分散微粒子 / 固液分離 / マイクロ・ナノ流体 / 誘電泳動 / クロスマッチ
Outline of Final Research Achievements

Although the cross-match test and the blood-typing test are basic, frequently-performed, and long-history tests, they are, mainly, still performed manually by technicians. In this study, we developed a device that automatically returns the test result 5 minutes after loading the sample and pushing a button, by exploiting microchip technologies and bubbles. The device also has features that the reaction result can be observed by naked eyes and that the device can detect the reaction even if the reaction is weak. Therefore, we concluded that the device performance is high in terms of both accuracy and detection speed.

Academic Significance and Societal Importance of the Research Achievements

医療従事者の過酷な労働環境が問題となっている昨今において、可能な限り労働者の負担を軽減することが重要となってきている。本研究では、基本的な血液検査を対象として、これまで技師が手動で30分程度を要して行ってきた検査を自動化することで労働者の負担軽減に貢献することができる。また、デバイスの操作は試薬のロードとボタン操作を行うのみというユーザーフレンドリーな設計とし、さらに検査結果も目視で容易に確認できる。さらに、検査に要する時間を5分程度に短縮し、検出可能な反応強度も大幅に改善した。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (8 results)

All 2019 2018 2017

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (7 results) (of which Int'l Joint Research: 4 results)

  • [Journal Article] Concentration-adjustable micromixers using droplet injection into a microchannel2019

    • Author(s)
      Ryosuke Sakurai, Ken Yamamoto, Masahiro Motosuke
    • Journal Title

      Analyst

      Volume: 144 Issue: 8 Pages: 2780-2787

    • DOI

      10.1039/c8an02310g

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] Bubble-based dilutor and slit-type RBC agglutination detector for an integrated microfluidic blood typing device using whole blood2018

    • Author(s)
      Ryosuke Sakurai, Ken Yamamoto, Masahiro Motosuke
    • Organizer
      Water on Materials Surface 2018 (WMS 2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Concentration-adjustable superdilutor chip2018

    • Author(s)
      Ken Yamamoto, Ryosuke Sakurai, Masahiro Motosuke
    • Organizer
      22nd International Conference on Miniaturized Systems for Chemistry and Life Science (MicroTAS 2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Whole-blood-based microfluidic blood typing chip with precise dilution and sensitive agglutination level detection2018

    • Author(s)
      Ryosuke Sakurai, Ken Yamamoto, Masahiro Motosuke
    • Organizer
      22nd International Conference on Miniaturized Systems for Chemistry and Life Science (MicroTAS 2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マイクロ流体デバイスにおける液滴の流動を利用した分散微粒子の輸送2017

    • Author(s)
      櫻井亮介,山本憲,元祐昌廣
    • Organizer
      日本混相流学会 混相流シンポジウム2017
    • Place of Presentation
      東京
    • Year and Date
      2017-08-19
    • Related Report
      2016 Research-status Report
  • [Presentation] Particle motions in microscale multiphase flow2017

    • Author(s)
      Ken Yamamoto, Ryosuke Sakurai, Masahiro Motosuke
    • Organizer
      International Conference on Materials and Systems for Sustainability 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] マイクロチャネルにおける液滴の流入を利用した懸濁液濃度の能動制御2017

    • Author(s)
      櫻井亮介、山本憲、元祐昌廣
    • Organizer
      化学とマイクロ・ナノシステム学会第36回研究会
    • Related Report
      2017 Research-status Report
  • [Presentation] マイクロ流体デバイスにおける液滴の流動を利用した分散微粒子の混合・希釈の促進2017

    • Author(s)
      櫻井亮介,山本憲,元祐昌廣
    • Organizer
      関東学生会 第56界学生員卒業研究発表講演会
    • Place of Presentation
      東京
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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