| Project/Area Number |
16K18174
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Civil and environmental engineering
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| Research Institution | University of Yamanashi (2017)
Ehime University (2016) |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 水生昆虫 / 環境DNA / メタバーコーディング / 河川生態 / NGS |
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| Outline of Final Research Achievements |
Environmental DNA (eDNA) is a powerful tool to investigate species-diversity of aquatic insects. In this study, first, I performed the meta-barcoding analysis of the eDNA originated from the aquatic insects. In result, most families which were collected from fields were detected from eDNA. The number of reads of each taxon were positively correlated with the abundance of insects from the fields. It supported that the eDNA analysis using NGS predict the abundance of freshwater insects. However, NGS analysis also detected high proportion of non-target taxa (e.g. microorganisms, crastaceans, inland insects). It is needed to design new universal primers targeted aquatic insects. Second, I analyzed the flow distance of eDNA originated from lentic algae. The concentration of the eDNA was decreased until about 3-7 km downstream from dams. It indicated that eDNA drifted down that distance in the study rivers.
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