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High quality microalgae cultivation with treated effluent based on lipid accumulation under nutrient depletion and exhaustion

Research Project

Project/Area Number 16K18178
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Civil and environmental engineering
Research InstitutionTottori University

Principal Investigator

TAKABE Yugo  鳥取大学, 工学研究科, 助教 (70625798)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords土着藻類 / 下水処理場 / 栄養塩 / 有機物組成 / 微細藻類 / 脂質 / エネルギー利用 / エネルギー生産 / 栄養塩欠乏
Outline of Final Research Achievements

Indigenous microalgae were cultivated using an actual treated effluent, and the temporal responses of cellular organic components to the variations in nitrogen concentration were investigated. Carbohydrate and lipid were accumulated as primary and secondary carbon and/or energy source, respectively, in indigenous microalgae against nitrogen depletion and exhaustion. Lipid reduction was observed after nitrogen replenishment; meanwhile, the time between the nitrogen replenishment and start of lipid reduction was varied according to the nitrogen concentrations in culture before the replenishment. Cultivation under nitrogen exhaustion was an attractive strategy for achieving higher lipid content. Meanwhile, to obtain indigenous microalgae with stable and high total lipid content, it is crucial to prevent increase in nitrogen concentrations in the reactor under the nitrogen exhaustion condition by measures such as controlling the hydraulic retention time.

Academic Significance and Societal Importance of the Research Achievements

人口減少等に伴う厳しい経営環境、また、将来の資源枯渇の懸念および地球温暖化防止を背景に、下水道施設では「排除・処理」から「活用・再生」への変換が求められている。そういった中で、下水中の栄養塩を活用した微細藻類培養・エネルギー化技術が、下水処理場のエネルギー供給拠点化実現に向けた中核技術として期待されている。本研究では、実下水処理水を用いた高油脂含有土着藻類培養を達成する上で重要となる培養システムにおける操作因子・管理項目の提案がなされ、現場への微細藻類培養・エネルギー化技術の導入を後押しするものと期待される。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (8 results)

All 2019 2018 2017

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (6 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Biochemical response of indigenous microalgal consortia to variations in nitrogen concentration of treated effluent2019

    • Author(s)
      Hironori Okazaki, Yugo Takabe, Takanori Masuda, Yoshiko Hoshikawa
    • Journal Title

      Bioresource Technology

      Volume: 280 Pages: 118-126

    • DOI

      10.1016/j.biortech.2019.02.014

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 下水を用いた微細藻類培養・バイオ燃料への変換2017

    • Author(s)
      高部祐剛
    • Journal Title

      水環境学会誌

      Volume: 40 Pages: 319-324

    • Related Report
      2017 Research-status Report
  • [Presentation] 窒素濃度変化が土着藻類での有機物の蓄積・分解に与える影響2019

    • Author(s)
      上村拓海、高部祐剛、岡崎広典、増田貴則、星川淑子
    • Organizer
      第53回日本水環境学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 下水処理水を用いた微細藻類培養における栄養塩濃度変化が脂質蓄積に及ぼす影響2018

    • Author(s)
      岡崎広典、高部祐剛、増田貴則、星川淑子
    • Organizer
      土木学会中国支部第70回研究発表会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Influence of nutrient concentrations in treated effluent on lipid content in indigenous microalgae2018

    • Author(s)
      Hironori Okazaki, Yugo Takabe, Takanori Masuda, Yoshiko Hoshikawa
    • Organizer
      WET2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Temporal changes of total lipid content in indigenous microalgae under abundant and scarce nutrient conditions2018

    • Author(s)
      Yugo Takabe, Hironori Okazaki, Takanori Masuda, Yoshiko Hoshikawa
    • Organizer
      IWA World Water Congress & Exhibition 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 下水処理水中栄養塩濃度変化が土着藻類での脂質蓄積・分解に与える影響2018

    • Author(s)
      高部祐剛、岡崎広典、増田貴則、星川淑子
    • Organizer
      日本生物工学会西日本支部第4回講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 下水処理水中栄養塩濃度変化に対する 土着藻類中脂質蓄積の応答の解明2017

    • Author(s)
      岡﨑広典、高部祐剛、増田貴則、星川淑子
    • Organizer
      第52回日本水環境学会年会
    • Related Report
      2017 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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