| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
In this study, I attempted to develop a technology for the purpose of promoter design, which is a key player in protein expression systems. In this research process, I analyzed the transcriptional regulatory mechanism in Aspergillus oryzae and succeeded in comprehensive identification of the binding site of some transcription factors. Furthermore, the influence of transcription factor binding sites in the promoter sequence on the transcriptional activity of downstream genes was shown mathematically. I also evaluated molecular tools using transcription factors and showed that it can be applied to various enzymatic analyses. The research findings including these results have been published in three English research papers and one Japanese review.
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